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+ | <style> | ||
+ | .floatsci{ | ||
+ | border:solid 0px red; | ||
+ | background:url("https://static.igem.org/mediawiki/2016/c/cb/%E6%80%BB%E7%BB%93%E5%9B%BE2_NUDT_CHINA.jpg"); | ||
+ | background-size:700px 394px; | ||
+ | width:700px; | ||
+ | height:394px; | ||
+ | } | ||
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+ | border:solid 0px red; | ||
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+ | </style> | ||
+ | <a id="a_href" href="/"> </a> | ||
+ | <script> | ||
+ | function jump_ink(url){ | ||
+ | var a_href=document.getElementById("a_href"); | ||
+ | a_href.href=url; | ||
+ | a_href.click(); | ||
+ | } | ||
+ | </script> | ||
+ | <div class="center"><div class="floatsci"> | ||
+ | |||
+ | <div id="div01_floatsci" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Results')"></div> | ||
+ | <div id="div02_floatsci" onclick="jump_ink('#PARTI')"></div> | ||
+ | <div id="div03_floatsci" onclick="jump_ink('#PARTII')"></div> | ||
+ | <div id="div04_floatsci" onclick="jump_ink('#PARTIII')"></div> | ||
+ | </div></div> | ||
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</p> | </p> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:16px;">:) Click boxes above to see our project & the practice. </span> | <span style="line-height:2;font-family:Perpetua;font-size:16px;">:) Click boxes above to see our project & the practice. </span> | ||
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a specific guide of our practice work this year, listed with the time they were conducted. With our project starting and ending, the HP went on. </span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a specific guide of our practice work this year, listed with the time they were conducted. With our project starting and ending, the HP went on. </span> | ||
</p> | </p> | ||
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+ | background:url("https://static.igem.org/mediawiki/2016/e/eb/Shijianzhou_NUDT_CHINA.jpg"); | ||
+ | background-size:700px 394px; | ||
+ | width:700px; | ||
+ | height:394px; | ||
+ | } | ||
+ | .floatink div{ | ||
+ | border:solid 0px red; | ||
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+ | top:53px; | ||
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+ | width:96px; | ||
+ | height:65px; | ||
+ | } | ||
+ | </style> | ||
+ | <div class="center"><div class="floatink"> | ||
+ | <div id="div01_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Description')"></div> | ||
+ | <div id="div02_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Design')"></div> | ||
+ | <div id="div03_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Results')"></div> | ||
+ | <div id="div04_floatink" onclick="jump_ink('#Interviews-with-Doctors')"></div> | ||
+ | <div id="div05_floatink" onclick="jump_ink('#sample-collection-for-wet-lab')"></div> | ||
+ | <div id="div06_floatink" onclick="jump_ink('#PARTIII')"></div> | ||
+ | <div id="div07_floatink" onclick="jump_ink('#cost-evaluation')"></div> | ||
+ | </div></div> | ||
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+ | <a name="PARTI"> </a> | ||
+ | </br> | ||
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</br> | </br> | ||
<h2 align="center" | <h2 align="center" | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Considering the fact that our project this year had a close relationship with cancer, and the aim of our project was to create a novel clinical application---handy, low-cost blood-microRNA detetion system, we attached great importance to medical professionalism. Thus we turned to doctors from First Xiangya Hospital affiliated to CSU, Second Xiangya Hospital affiliated to CSU and Health Center of NUDT as our medical professional consultant in the whole process of the project.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Considering the fact that our project this year had a close relationship with cancer, and the aim of our project was to create a novel clinical application---handy, low-cost blood-microRNA detetion system, we attached great importance to medical professionalism. Thus we turned to doctors from First Xiangya Hospital affiliated to CSU, Second Xiangya Hospital affiliated to CSU and Health Center of NUDT as our medical professional consultant in the whole process of the project.</span> | ||
</p> | </p> | ||
+ | <a name="Interviews-with-Doctors"> </a> | ||
+ | </br> | ||
</br> | </br> | ||
<h3> | <h3> | ||
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</br> | </br> | ||
<h4> | <h4> | ||
− | <span style="color:#7f1015">1.Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span> | + | <span style="color:#7f1015">1. Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span> |
</h4> | </h4> | ||
− | + | </br> | |
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)Procedure of Current Cancer Detection</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Procedure of Current Cancer Detection</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Gap Exists Between Clinical Application and Basic Research </span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Gap Exists Between Clinical Application and Basic Research </span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">There was distance between clinical application and basic research in early detection of cancer. For instance, some means of early cancer detection still had a long way to go before proved efficacious---commonly 5-10 years experimental validation phase is integral to clinical application.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">There was distance between clinical application and basic research in early detection of cancer. For instance, some means of early cancer detection still had a long way to go before proved efficacious---commonly 5-10 years experimental validation phase is integral to clinical application.</span></br> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3)Stability of MiRNA in Blood is Doubted</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) Stability of MiRNA in Blood is Doubted</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(4)Efficiency of MiRNA Detection is Not Guaranteed</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(4) Efficiency of MiRNA Detection is Not Guaranteed</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(5)Prospect of MiRNA Is Expected</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(5) Prospect of MiRNA Is Expected</span> |
</p> | </p> | ||
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<h4> | <h4> | ||
− | <span style="color:#7f1015">2.Dr.Hou Tao, Oncology Department,Second Xiangya Affiliated Hospital of CSU</span> | + | <span style="color:#7f1015">2. Dr.Hou Tao, Oncology Department,Second Xiangya Affiliated Hospital of CSU</span> |
</h4></span> | </h4></span> | ||
</h4> | </h4> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)Current situation of tumor biomarker test</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Current situation of tumor biomarker test</span> |
</p> | </p> | ||
− | + | ||
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We asked Dr.Hou about some details about current tumor biomarker test the hospital usuallly suggested patients to take. And this helped us learn more about the current situation of tumor biomarker test.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We asked Dr.Hou about some details about current tumor biomarker test the hospital usuallly suggested patients to take. And this helped us learn more about the current situation of tumor biomarker test.</span> | ||
</p> | </p> | ||
− | + | ||
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.Biomarker tests in health exams</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Biomarker tests in health exams</span> |
</p> | </p> | ||
− | + | ||
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In a normal health exam, there is an optional choice of tumor biomarker test including tests for 12 biomarkers of most frequent cancers, which is called ‘set 12’ by doctors. Talking about taking the biomarker tests, Dr.Hou said that the popularity of biomarker tests was increasing in oncology department in hospitals in China. And with more people getting familiar with tumor biomarker test, it was gaining increasing attention, and a great number of healthy people requested tumor biomarker test in their health exams, especially the elder. Tumor biomarker tests are gaining increasing attention from the crowd.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In a normal health exam, there is an optional choice of tumor biomarker test including tests for 12 biomarkers of most frequent cancers, which is called ‘set 12’ by doctors. Talking about taking the biomarker tests, Dr.Hou said that the popularity of biomarker tests was increasing in oncology department in hospitals in China. And with more people getting familiar with tumor biomarker test, it was gaining increasing attention, and a great number of healthy people requested tumor biomarker test in their health exams, especially the elder. Tumor biomarker tests are gaining increasing attention from the crowd.</span> | ||
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</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.Details of a normal biomarker test in health exams</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. Details of a normal biomarker test in health exams</span> |
</p> | </p> | ||
− | + | ||
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Contents of ‘set 12’ biomarker test in a normal health exam:</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Contents of ‘set 12’ biomarker test in a normal health exam:</span> | ||
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<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:414.2pt;"> | + | <table class="MsoTableGrid" style="width:414.2pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Cost of the test for 12 biomarkers, or the ‘set 12’ is ¥500, including biomarkers of breast cancer, liver cancer and so on. Dr.Hou also told us that the validness of biomarkers was not so optimistic, thus could only work as a reference for diagnosis of cancers.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Cost of the test for 12 biomarkers, or the ‘set 12’ is ¥500, including biomarkers of breast cancer, liver cancer and so on. Dr.Hou also told us that the validness of biomarkers was not so optimistic, thus could only work as a reference for diagnosis of cancers.</span> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Advice for The Project---SPLIT-GFP, SPLIT LUCIFERASE, OR SPLIT HRP?</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Advice for The Project---SPLIT-GFP, SPLIT LUCIFERASE, OR SPLIT HRP?</span> |
</p> | </p> | ||
</br> | </br> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">To improve the sensitivity and specificity of RCA output signal and to visualize the RCA outputs in the wet-lab experiments, we designed a single guide RNA mediated dCas9 binding system and a conjugated reporting system. However, dilemma came that split-luciferase, split-HRP and split-GFP all seemed to meet our requirements of reporting the signal. Which was the best fit? </span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">To improve the sensitivity and specificity of RCA output signal and to visualize the RCA outputs in the wet-lab experiments, we designed a single guide RNA mediated dCas9 binding system and a conjugated reporting system. However, dilemma came that split-luciferase, split-HRP and split-GFP all seemed to meet our requirements of reporting the signal. Which was the best fit? </span> | ||
</p> | </p> | ||
− | + | ||
</p> | </p> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we searched for information of the three reporting systems:</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we searched for information of the three reporting systems:</span> | ||
</p> | </p> | ||
− | <table class="MsoNormalTable" align="center" style="width:435.85pt;"> | + | <table class="MsoNormalTable" align="center" style="width:435.85pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" align="center" style="width:526.95pt;"> | + | <table class="MsoTableGrid" align="center" style="width:526.95pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Conclusion drawn from the result:</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Conclusion drawn from the result:</span> | ||
</p> | </p> | ||
− | + | </br> | |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.Costs of current early cancer detection methods vary largely, but on the whole the price is not low, which may make it hard for poor families to afford. Plus, it may also turn down people’s positivity to take early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Costs of current early cancer detection methods vary largely, but on the whole the price is not low, which may make it hard for poor families to afford. Plus, it may also turn down people’s positivity to take early cancer detection.</span> |
</p> | </p> | ||
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.These test methods have high need of good external condition, eg. Electricity, instruments, operation environment. These may restrict the early cancer detection in big cities or counties, leaving remote area aside.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. These test methods have high need of good external condition, eg. Electricity, instruments, operation environment. These may restrict the early cancer detection in big cities or counties, leaving remote area aside.</span> |
</p> | </p> | ||
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Popularity of these test methods is low, which we can see from the result that people almost can take early cancer detection only in developed cities or counties. This makes it hard for people in remote areas, such as rural districts and behindhand villages, where cancer occurrence rate is also high and cancer death rate is even higher because of backward medical services.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Popularity of these test methods is low, which we can see from the result that people almost can take early cancer detection only in developed cities or counties. This makes it hard for people in remote areas, such as rural districts and behindhand villages, where cancer occurrence rate is also high and cancer death rate is even higher because of backward medical services.</span> |
</p> | </p> | ||
</br> | </br> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for. </span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for. </span> | ||
</p> | </p> | ||
+ | <a name="sample-collection-for-wet-lab"> </a> | ||
+ | </br> | ||
</br> | </br> | ||
<h3> | <h3> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Approve of the Informed Consent Form</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Approve of the Informed Consent Form</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3.Meeting with sample groups</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Meeting with sample groups</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4.Processes of sample gathering</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4. Processes of sample gathering</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Doctors collected 5L blood sample/person with coagulation tube and match each with the person’s information(including name, age and extra information as AD, stages of NSCLC, etc from cancer patients).</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Doctors collected 5L blood sample/person with coagulation tube and match each with the person’s information(including name, age and extra information as AD, stages of NSCLC, etc from cancer patients).</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">d.Samples transmitted to professional operators and serum was separated.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">d. Samples transmitted to professional operators and serum was separated.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">e.Registering samples in the information management system and setting up links with the Informed Consent Form.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">e. Registering samples in the information management system and setting up links with the Informed Consent Form.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">f.Sample serum was transmitted to cryopreserve tubes and placed in -80℃ in the lab.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">f. Sample serum was transmitted to cryopreserve tubes and placed in -80℃ in the lab.</span> |
</p> | </p> | ||
− | </ | + | <a name="PARTII"> </a> |
− | + | </br></br> | |
<h2 align="center" | <h2 align="center" | ||
> | > | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span> | ||
− | </p> | + | </p><a name="cost-evaluation"> </a> |
</br> | </br> | ||
+ | </br> | ||
+ | |||
<h3> | <h3> | ||
<span style="color:#7f1015">Cost Evaluation</span> | <span style="color:#7f1015">Cost Evaluation</span> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The other reason we made this cost evaluation was to make it a feedback for the project and find other aspects it may develop itself in.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The other reason we made this cost evaluation was to make it a feedback for the project and find other aspects it may develop itself in.</span> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
</p> | </p> | ||
</br> | </br> | ||
<!--此处开始链接--> | <!--此处开始链接--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Click here to view details of contents, instrument, reagents and experimental procedure of our product | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The following is the evaluation of our expected product cost.(Click here to view details of contents, instrument, reagents and experimental procedure of our potential product.)</span> |
</p> | </p> | ||
+ | |||
+ | |||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Project Contents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Project Contents</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Major Instruments and Reagents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Major Instruments and Reagents</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Instruments</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Instruments</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Reagents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Reagents</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Experimental Procedure</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Experimental Procedure</span> |
</p> | </p> | ||
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</p> | </p> | ||
</br> | </br> | ||
− | <!--链接结束--> | + | <!--链接结束--> |
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Cost Evaluation</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Cost Evaluation</span> |
</p> | </p> | ||
</br> | </br> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:426.1pt;"> | + | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:426.1pt;"> | + | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2.Interpretation of Result</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Interpretation of Result</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)The total price of our project is around $3.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) The total price of our project is around $3.</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Comparisons with some other quantitative miRNA detection methods.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Comparisons with some other quantitative miRNA detection methods.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. miRNA Real-time qPCR</span> |
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | <p style="text-align:center;"> | ||
+ | <br /> | ||
</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoNormalTable" style="width:414.95pt;"> | + | <table class="MsoNormalTable" style="text-align:center;width:414.95pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td width="249" valign="center" style="border: | + | <td width="249" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">project</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">project</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="243" valign="center" colspan="2" style="border: | + | <td width="243" valign="center" colspan="2" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">contents</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">contents</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="189" valign="center" style="border: | + | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
− | <b><span style="font-family:Arial;color:# | + | <b><span style="font-family:Arial;color:#000000;font-size:10.5pt;">price</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> |
</p> | </p> | ||
</td> | </td> | ||
− | <td width="147" valign="center" style="border: | + | <td width="147" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">period</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">period</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="249" valign="center" rowspan="3" style="border: | + | <td width="249" valign="center" rowspan="3" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">miRNA SYBR Green I Relative quantification</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">miRNA SYBR Green I Relative quantification</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="102" valign="center" style="border: | + | <td width="102" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Basic cost</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Basic cost</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="140" valign="center" style="border: | + | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Primer synthesis</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Primer synthesis</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="189" valign="center" style="border: | + | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
− | <b><span style="font-family:Arial;color:# | + | <b><span style="font-family:Arial;color:#000000;font-size:10.5pt;">$223.2/miRNA</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> |
</p> | </p> | ||
</td> | </td> | ||
− | <td width="147" valign="center" rowspan="3" style="border: | + | <td width="147" valign="center" rowspan="3" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">4-5weeks</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">4-5weeks</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="102" valign="center" rowspan="2" style="border: | + | <td width="102" valign="center" rowspan="2" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Sample detection fee</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Sample detection fee</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="140" valign="center" style="border: | + | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;"><</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;"><</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="189" valign="center" style="border: | + | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
− | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:# | + | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:#000000;">$44.6/sample</span><span style="font-family:Arial;color:#000000;">·</span><span style="font-family:Arial;color:#000000;">gene</span></span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> |
</p> | </p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="140" valign="center" style="border: | + | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
<span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;">≥</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | <span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;">≥</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
</p> | </p> | ||
</td> | </td> | ||
− | <td width="189" valign="center" style="border: | + | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> |
<p class="MsoNormal" align="center" style="text-align:center;"> | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
− | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:# | + | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:#000000;">$29.8/sample</span><span style="font-family:Arial;color:#000000;">·</span><span style="font-family:Arial;color:#000000;">gene</span></span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> |
</p> | </p> | ||
</td> | </td> | ||
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</tbody> | </tbody> | ||
</table> | </table> | ||
− | < | + | <br /> |
+ | <br /> | ||
+ | </p> | ||
+ | <div style="text-align:center;"> | ||
+ | <span style="line-height:1.5;"></span> | ||
+ | </div> | ||
+ | <p> | ||
+ | <br /> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">miRNA CHIP</span> |
</p> | </p> | ||
− | <table class="MsoTableGrid" style="width:414.6pt;"> | + | <table class="MsoTableGrid" style="width:414.6pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Our product-to-be costs only $3 in each round, it has an overwhelming advantage in cost.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.The reporting system of our project is the split-HRP, which satisfied the need of a new visible signal showing method. Thus, we eliminated heavy instruments and strict environment requests, finally making it possible to put it into wide use in remote areas which lack qualified conditions for current early cancer detection methods.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. The reporting system of our project is the split-HRP, which satisfied the need of a new visible signal showing method. Thus, we eliminated heavy instruments and strict environment requests, finally making it possible to put it into wide use in remote areas which lack qualified conditions for current early cancer detection methods.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Our project is expected to solve the problem of lack of early cancer detection methods in remote areas as well as places bad in medical conditions.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Our project is expected to solve the problem of lack of early cancer detection methods in remote areas as well as places bad in medical conditions.</span> |
− | </p> | + | </p><a name="PARTIII"> </a> |
+ | </br> | ||
</br> | </br> | ||
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<!---此处链接---> | <!---此处链接---> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The survey mainly involved the three aspects: | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The survey mainly involved the three aspects:</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)How much the public knew about early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) How much the public knew about early cancer detection.</span> |
</p> | </p> | ||
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<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)What their main problems were when seeking for early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) What their main problems were when seeking for early cancer detection.</span> |
</p> | </p> | ||
</br> | </br> | ||
</html> | </html> | ||
− | [[File: | + | [[File:图片2_NUDT_CHINA.jpg|700px|center]] |
<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3)To publize knowledge of cancer,early cancer detection and our project</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) To publize knowledge of cancer,early cancer detection and our project</span> |
</p> | </p> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">a. What do you think of our project?</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. What do you think of our project?</span> | ||
</p> | </p> | ||
− | + | </html> | |
[[File:diaocha3_NUDT_CHINA.jpg|700px|center]] | [[File:diaocha3_NUDT_CHINA.jpg|700px|center]] | ||
<html> | <html> | ||
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<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Do you expect to see our product put in to market?</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Do you expect to see our product put in to market?</span> |
</p> | </p> | ||
</html> | </html> | ||
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[[File:社区1_NUDT_CHINA.jpg|700px|center]] | [[File:社区1_NUDT_CHINA.jpg|700px|center]] | ||
<html> | <html> | ||
− | + | </br></br> | |
</html> | </html> | ||
[[File:社区2_NUDT_CHINA.jpg|700px|center]] | [[File:社区2_NUDT_CHINA.jpg|700px|center]] | ||
− | <html> | + | <html></br></br> |
</html> | </html> | ||
[[File:社区3_NUDT_CHINA.jpg|700px|center]] | [[File:社区3_NUDT_CHINA.jpg|700px|center]] | ||
− | <html> | + | <html></br></br> |
</html> | </html> | ||
[[File:社区4_NUDT_CHINA.jpg|700px|center]] | [[File:社区4_NUDT_CHINA.jpg|700px|center]] | ||
− | <html> | + | <html></br></br> |
<h3> | <h3> | ||
− | <span style="color:#7f1015">Lab | + | <span style="color:#7f1015">Lab Day For High School Students </span> |
</h3> | </h3> | ||
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[[File:实验室1_NUDT_CHINA.jpg|700px|center]] | [[File:实验室1_NUDT_CHINA.jpg|700px|center]] | ||
<html> | <html> | ||
− | + | </br></br> | |
</html> | </html> | ||
[[File:实验室2_NUDT_CHINA.jpg|700px|center]] | [[File:实验室2_NUDT_CHINA.jpg|700px|center]] | ||
− | <html> | + | <html></br></br> |
</html> | </html> | ||
[[File:实验室3_NUDT_CHINA.jpg|700px|center]] | [[File:实验室3_NUDT_CHINA.jpg|700px|center]] | ||
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</br> | </br> | ||
<h3> | <h3> | ||
− | <span style="color:#7f1015">Fun | + | <span style="color:#7f1015">Fun Game with Our Topic</span> |
</h3> | </h3> | ||
+ | </br</br><p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | </br><video style="width:100%;" controls="controls" preload="auto"> | ||
+ | <source src="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4" type="video/mp4" /> | ||
+ | Your browser does not support the video tag. Please click <a href="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4">HERE</a> to open this video! | ||
+ | </video> | ||
+ | |||
+ | |||
+ | |||
+ | <a href="">https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4</a> | ||
− | |||
− | |||
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<html> | <html> | ||
<p> | <p> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:16px;">mini poster for everyone</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:16px;">This is the mini poster about knowledge of cancer and early cancer detection for everyone. We put it on small fans and gave them away to the crowd.</span> |
</p> | </p> | ||
− | + | </br> | |
</html> | </html> | ||
[[File:发扇子_NUDT_CHINA.jpg|700px|center]] | [[File:发扇子_NUDT_CHINA.jpg|700px|center]] |
Latest revision as of 03:54, 20 October 2016
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