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− | <div class="center"><div class=" | + | <div class="center"><div class="floatsci"> |
− | <div id=" | + | <div id="div01_floatsci" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Results')"></div> |
− | <div id=" | + | <div id="div02_floatsci" onclick="jump_ink('#PARTI')"></div> |
− | <div id=" | + | <div id="div03_floatsci" onclick="jump_ink('#PARTII')"></div> |
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+ | <a name="PARTI"> </a> | ||
</br> | </br> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Considering the fact that our project this year had a close relationship with cancer, and the aim of our project was to create a novel clinical application---handy, low-cost blood-microRNA detetion system, we attached great importance to medical professionalism. Thus we turned to doctors from First Xiangya Hospital affiliated to CSU, Second Xiangya Hospital affiliated to CSU and Health Center of NUDT as our medical professional consultant in the whole process of the project.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Considering the fact that our project this year had a close relationship with cancer, and the aim of our project was to create a novel clinical application---handy, low-cost blood-microRNA detetion system, we attached great importance to medical professionalism. Thus we turned to doctors from First Xiangya Hospital affiliated to CSU, Second Xiangya Hospital affiliated to CSU and Health Center of NUDT as our medical professional consultant in the whole process of the project.</span> | ||
</p> | </p> | ||
+ | <a name="Interviews-with-Doctors"> </a> | ||
+ | </br> | ||
</br> | </br> | ||
<h3> | <h3> | ||
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</br> | </br> | ||
<h4> | <h4> | ||
− | <span style="color:#7f1015">1.Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span> | + | <span style="color:#7f1015">1. Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span> |
</h4> | </h4> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)Procedure of Current Cancer Detection</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Procedure of Current Cancer Detection</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Gap Exists Between Clinical Application and Basic Research </span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Gap Exists Between Clinical Application and Basic Research </span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
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</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3)Stability of MiRNA in Blood is Doubted</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) Stability of MiRNA in Blood is Doubted</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(4)Efficiency of MiRNA Detection is Not Guaranteed</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(4) Efficiency of MiRNA Detection is Not Guaranteed</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(5)Prospect of MiRNA Is Expected</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(5) Prospect of MiRNA Is Expected</span> |
</p> | </p> | ||
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<h4> | <h4> | ||
− | <span style="color:#7f1015">2.Dr.Hou Tao, Oncology Department,Second Xiangya Affiliated Hospital of CSU</span> | + | <span style="color:#7f1015">2. Dr.Hou Tao, Oncology Department,Second Xiangya Affiliated Hospital of CSU</span> |
</h4></span> | </h4></span> | ||
</h4> | </h4> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)Current situation of tumor biomarker test</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Current situation of tumor biomarker test</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.Biomarker tests in health exams</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Biomarker tests in health exams</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.Details of a normal biomarker test in health exams</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. Details of a normal biomarker test in health exams</span> |
</p> | </p> | ||
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<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:414.2pt;"> | + | <table class="MsoTableGrid" style="width:414.2pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Advice for The Project---SPLIT-GFP, SPLIT LUCIFERASE, OR SPLIT HRP?</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Advice for The Project---SPLIT-GFP, SPLIT LUCIFERASE, OR SPLIT HRP?</span> |
</p> | </p> | ||
</br> | </br> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we searched for information of the three reporting systems:</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we searched for information of the three reporting systems:</span> | ||
</p> | </p> | ||
− | <table class="MsoNormalTable" align="center" style="width:435.85pt;"> | + | <table class="MsoNormalTable" align="center" style="width:435.85pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" align="center" style="width:526.95pt;"> | + | <table class="MsoTableGrid" align="center" style="width:526.95pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.Costs of current early cancer detection methods vary largely, but on the whole the price is not low, which may make it hard for poor families to afford. Plus, it may also turn down people’s positivity to take early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Costs of current early cancer detection methods vary largely, but on the whole the price is not low, which may make it hard for poor families to afford. Plus, it may also turn down people’s positivity to take early cancer detection.</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.These test methods have high need of good external condition, eg. Electricity, instruments, operation environment. These may restrict the early cancer detection in big cities or counties, leaving remote area aside.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. These test methods have high need of good external condition, eg. Electricity, instruments, operation environment. These may restrict the early cancer detection in big cities or counties, leaving remote area aside.</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Popularity of these test methods is low, which we can see from the result that people almost can take early cancer detection only in developed cities or counties. This makes it hard for people in remote areas, such as rural districts and behindhand villages, where cancer occurrence rate is also high and cancer death rate is even higher because of backward medical services.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Popularity of these test methods is low, which we can see from the result that people almost can take early cancer detection only in developed cities or counties. This makes it hard for people in remote areas, such as rural districts and behindhand villages, where cancer occurrence rate is also high and cancer death rate is even higher because of backward medical services.</span> |
</p> | </p> | ||
</br> | </br> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for. </span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for. </span> | ||
</p> | </p> | ||
− | </br></br> | + | <a name="sample-collection-for-wet-lab"> </a> |
+ | </br> | ||
+ | </br> | ||
<h3> | <h3> | ||
<span style="color:#7f1015">Sample Collection for Wet Lab</span> | <span style="color:#7f1015">Sample Collection for Wet Lab</span> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Approve of the Informed Consent Form</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Approve of the Informed Consent Form</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3.Meeting with sample groups</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Meeting with sample groups</span> |
</p> | </p> | ||
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</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4.Processes of sample gathering</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4. Processes of sample gathering</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Doctors collected 5L blood sample/person with coagulation tube and match each with the person’s information(including name, age and extra information as AD, stages of NSCLC, etc from cancer patients).</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Doctors collected 5L blood sample/person with coagulation tube and match each with the person’s information(including name, age and extra information as AD, stages of NSCLC, etc from cancer patients).</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">d.Samples transmitted to professional operators and serum was separated.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">d. Samples transmitted to professional operators and serum was separated.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">e.Registering samples in the information management system and setting up links with the Informed Consent Form.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">e. Registering samples in the information management system and setting up links with the Informed Consent Form.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">f.Sample serum was transmitted to cryopreserve tubes and placed in -80℃ in the lab.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">f. Sample serum was transmitted to cryopreserve tubes and placed in -80℃ in the lab.</span> |
</p> | </p> | ||
+ | <a name="PARTII"> </a> | ||
</br></br> | </br></br> | ||
− | |||
<h2 align="center" | <h2 align="center" | ||
> | > | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span> | ||
− | </p> | + | </p><a name="cost-evaluation"> </a> |
</br> | </br> | ||
+ | </br> | ||
+ | |||
<h3> | <h3> | ||
<span style="color:#7f1015">Cost Evaluation</span> | <span style="color:#7f1015">Cost Evaluation</span> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Project Contents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Project Contents</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2.Major Instruments and Reagents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Major Instruments and Reagents</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)Instruments</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Instruments</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Reagents</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Reagents</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3.Experimental Procedure</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Experimental Procedure</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Cost Evaluation</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Cost Evaluation</span> |
</p> | </p> | ||
</br> | </br> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:426.1pt;"> | + | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoTableGrid" style="width:426.1pt;"> | + | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2.Interpretation of Result</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Interpretation of Result</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)The total price of our project is around $3.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) The total price of our project is around $3.</span> |
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)Comparisons with some other quantitative miRNA detection methods.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Comparisons with some other quantitative miRNA detection methods.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.miRNA Real-time qPCR</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. miRNA Real-time qPCR</span> |
</p> | </p> | ||
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</p> | </p> | ||
<p> | <p> | ||
− | <table class="MsoNormalTable" style="text-align:center;width:414.95pt;"> | + | <table class="MsoNormalTable" style="text-align:center;width:414.95pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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</p> | </p> | ||
+ | |||
− | |||
</p> | </p> | ||
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</p> | </p> | ||
− | <table class="MsoTableGrid" style="width:414.6pt;"> | + | <table class="MsoTableGrid" style="width:414.6pt;margin:auto;"> |
<tbody> | <tbody> | ||
<tr> | <tr> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a.Our product-to-be costs only $3 in each round, it has an overwhelming advantage in cost.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Our product-to-be costs only $3 in each round, it has an overwhelming advantage in cost.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b.The reporting system of our project is the split-HRP, which satisfied the need of a new visible signal showing method. Thus, we eliminated heavy instruments and strict environment requests, finally making it possible to put it into wide use in remote areas which lack qualified conditions for current early cancer detection methods.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. The reporting system of our project is the split-HRP, which satisfied the need of a new visible signal showing method. Thus, we eliminated heavy instruments and strict environment requests, finally making it possible to put it into wide use in remote areas which lack qualified conditions for current early cancer detection methods.</span> |
</p> | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Our project is expected to solve the problem of lack of early cancer detection methods in remote areas as well as places bad in medical conditions.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Our project is expected to solve the problem of lack of early cancer detection methods in remote areas as well as places bad in medical conditions.</span> |
− | </p> | + | </p><a name="PARTIII"> </a> |
+ | </br> | ||
</br> | </br> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)How much the public knew about early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) How much the public knew about early cancer detection.</span> |
</p> | </p> | ||
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<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)What their main problems were when seeking for early cancer detection.</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) What their main problems were when seeking for early cancer detection.</span> |
</p> | </p> | ||
</br> | </br> | ||
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<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3)To publize knowledge of cancer,early cancer detection and our project</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) To publize knowledge of cancer,early cancer detection and our project</span> |
</p> | </p> | ||
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<html> | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Do you expect to see our product put in to market?</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Do you expect to see our product put in to market?</span> |
</p> | </p> | ||
</html> | </html> | ||
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<h3> | <h3> | ||
− | <span style="color:#7f1015">Lab | + | <span style="color:#7f1015">Lab Day For High School Students </span> |
</h3> | </h3> | ||
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</br> | </br> | ||
<h3> | <h3> | ||
− | <span style="color:#7f1015">Fun | + | <span style="color:#7f1015">Fun Game with Our Topic</span> |
</h3> | </h3> | ||
+ | </br</br><p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | </br><video style="width:100%;" controls="controls" preload="auto"> | ||
+ | <source src="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4" type="video/mp4" /> | ||
+ | Your browser does not support the video tag. Please click <a href="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4">HERE</a> to open this video! | ||
+ | </video> | ||
+ | |||
+ | |||
+ | |||
+ | <a href="">https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4</a> | ||
− | |||
− | |||
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<html> | <html> | ||
<p> | <p> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:16px;">mini poster for everyone</span> | + | <span style="line-height:2;font-family:Perpetua;font-size:16px;">This is the mini poster about knowledge of cancer and early cancer detection for everyone. We put it on small fans and gave them away to the crowd.</span> |
</p> | </p> | ||
− | + | </br> | |
</html> | </html> | ||
[[File:发扇子_NUDT_CHINA.jpg|700px|center]] | [[File:发扇子_NUDT_CHINA.jpg|700px|center]] |
Latest revision as of 03:54, 20 October 2016
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