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Revision as of 18:22, 1 December 2016

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Transformation

Requirements for each sample

  •   1 tube of thawed chemically competent cells (keep covered in ice at all times).
  •   1-10 uL ligation product cloned in plasmid.
  •   250 uL SOC
  •  1 LB plate with appropriate antibiotics