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− | <!--{{UrbanTundra_Edmonton}}-->
| + | {{UrbanTundra_Edmonton/Main-CSS}} |
− | {{UrbanTundra_Edmonton/ResearchPages}} | + | {{UrbanTundra_Edmonton/Header}} |
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− | <head> | + | <div id="ute-services-section"> |
− | <style> | + | <div class="container"> |
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| + | <!--NAV LINKS--> |
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− | .timeline > li:after { | + | <div class="side-nav-container"> |
− | content: " ";
| + | <ul style="list-style-type: none;"> |
− | display: table;
| + | <li class="side-nav-toplink">TEAM</li> |
− | } | + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Our_Story">Our Story</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Team">Team</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Collaborations">Collaborations</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">PROJECT</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Description">Background</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Overview">Overview</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Design">Gene Design</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Experiments">Experimental</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Proof">Bio Reaction</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Demonstrate">O<sub>2</sub></a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Notebook">Notebook</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">PARTS</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Parts">BioBrick</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Composite_Part">CLD-</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Part_Collection">Collection</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">SAFETY</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Lab_Safety">Lab Safety</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Protocals">Protocols</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">ATTRIBUTIONS</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Attributions">Support</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/FullCitations">Citations</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">HUMAN PRACTICES</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Integrated_Practices">Integrated Practices</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/BroaderApplications">Broader Applications</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Engagement">Outreach</a></li> |
| + | </ul> |
| + | <li class="side-nav-toplink">ACHIEVEMENTS</li> |
| + | <ul class="sublist"> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/HP/Silver">Silver</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/HP/Gold">Gold</a></li> |
| + | <li><a href="https://2016.igem.org/Team:UrbanTundra_Edmonton/Awards">Awards</a></li> |
| + | </ul> |
| + | </ul> |
| + | </div> |
| + | </div> |
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− | <div class="container">
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− | <div class="page-header">
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− | <h1 id="timeline">Timeline</h1>
| + | |
− | </div>
| + | |
− | <ul class="timeline">
| + | |
− | <li>
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− | <div class="timeline-badge"><i class="glyphicon glyphicon-check"></i></div>
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− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">July 20<sup>th</sup> 2016</h4>
| + | |
− | </div>
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− | <div class="timeline-body">
| + | |
− | <p>
| + | |
− | <ol>
| + | |
− | <li>4 microlitres of milliq water with 1 microlitre of plasmid</li>
| + | |
− | <li>5 microlitres of no chlorite dismutase</li>
| + | |
− | <li>1 microlitre of cutsmart buffer (specifically 1.1111 microlitre)</li>
| + | |
− | <ul>a. 10 x cutsmart buffer into tubes makes 1x [ ]</ul>
| + | |
− | <li>When adding BsaI, only touch the surface of the BsaI to the pipette tip. The reason: has glycerol which is sticky. Take 0.5 microlitre of BsaI.</li>
| + | |
− | <li>Mix sample very gently then put into a 37 degrees celsius incubator for 1 hour. </li>
| + | |
− | <li>Heat kill at 65 degrees celsius for 20 minutes. This kills the BsaI to prevent star activity. </li>
| + | |
− | <li>Pulse down tubes then add 8 microlitre of milliq water. </li>
| + | |
− | <li>Add 2 microlitres of T4DNA ligase buffer. </li>
| + | |
− | <li>Add 0.5 microlitre of ligase then mix gently and incubate at room temperature for 30 minutes or longer </li>
| + | |
− | </ol>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-badge warning"><i class="glyphicon glyphicon-credit-card"></i></div>
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− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">July 21<sup>st</sup> 2016</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>
| + | |
− | <ol>
| + | |
− | <li>Did some plating. </li>
| + | |
− | <li>Plated three agar types:</li>
| + | |
− | <ul>A. 4 plates of no antibiotic </ul>
| + | |
− | <ul>B. 10 plates of kanamycin (CM)</ul>
| + | |
− | <ul>C. 10 plates of ampicillin (Amp)</ul>
| + | |
− | <li>Purpose: try to find the antibiotic marker on bacteria.</li>
| + | |
− | <li>Observational expectations: If it’s purple, then there is no Cld gene sequence. If white then has CLd gene sequence. </li>
| + | |
− | <li>Lives or dies on certain plates. Alive on ampicillin, then it has the marker. Dies it has the CM marker. </li>
| + | |
− | <li>Added 20 microlitres in each plate</li>
| + | |
− | <ul>A. A total of 7 plates</ul>
| + | |
− | <ul>B. DNA minus has none, CM, and AMP</ul>
| + | |
− | <ul>C. Positive control has CM and AMP</ul>
| + | |
− | <ul>D. Cld +/- has CM and AMP</ul>
| + | |
− | </ol>
| + | |
− | <h4>Competent cell transformation:</h4>
| + | |
− | <ol>
| + | |
− | <li>1 microlitre of positive control added to competent cells (positive control: CPB we used yesterday before cutting and gluing)</li>
| + | |
− | <li>10 microlitre of cloned plasmid added to competent cells (Cloned plasmid is what we did yesterday).</li>
| + | |
− | <li>Positive control, negative control, and cloned plasmid were chilled for 30 minutes, heat shocked for 90 seconds, chilled on ice for 5 minutes, and finally incubated at 37 degrees celsius with 1 ml of LB broth.</li>
| + | |
− | </ol>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
| + | |
− | <li>
| + | |
− | <div class="timeline-badge danger"><i class="glyphicon glyphicon-credit-card"></i></div>
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− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">July 22<sup>nd</sup> 2016</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>
| + | |
− |
| + | |
− | <table style="width:96%">
| + | |
− | <tr>
| + | |
− | <th></th>
| + | |
− | <th>+ control</th>
| + | |
− | <th>DNA -</th>
| + | |
− | <th>Cld +</th>
| + | |
− | <th>Cld -</th>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>No Antibiotic</td>
| + | |
− | <td></td>
| + | |
− | <td>Yes, very thick</td>
| + | |
− | <td></td>
| + | |
− | <td></rd>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Amp</td>
| + | |
− | <td>No growth</td>
| + | |
− | <td>No growth</td>
| + | |
− | <td>No growth</td>
| + | |
− | <td>No growth</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>CM</td>
| + | |
− | <td>Growth (purple colonies)</td>
| + | |
− | <td>No growth</td>
| + | |
− | <td>Possibly purple and white*</td>
| + | |
− | <td>Possible purple and white*</td>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | * Purple colour will develop more over larger period of time (perhaps over the weekend)
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </li>
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− | <li class="timeline-inverted">
| + | |
− | <div class="timeline-panel">
| + | |
− | <div class="timeline-heading">
| + | |
− | <h4 class="timeline-title">Mussum ipsum cacilds</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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− | <h4 class="timeline-title">Mussum ipsum cacilds</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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− | <hr>
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− | <div class="btn-group">
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− | </button>
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− | <li><a href="#">Action</a></li>
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− | <li><a href="#">Another action</a></li>
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− | <li><a href="#">Something else here</a></li>
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− | <li class="divider"></li>
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− | <li><a href="#">Separated link</a></li>
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− | <h4 class="timeline-title">Mussum ipsum cacilds</h4>
| + | |
− | </div>
| + | |
− | <div class="timeline-body">
| + | |
− | <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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− | <h4 class="timeline-title">Mussum ipsum cacilds</h4>
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− | </div>
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− | <p>Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.</p>
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| + | <h1>Lab Notebook</h1> |
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| + | <h3 style:"text-align: center"><a href="https://static.igem.org/mediawiki/2016/b/b8/T--UrbanTundra_Edmonton--JuneNotebook_.pdf" target="_blank">June</a></h3> |
| + | <h3 style:"text-align: center"><a href="https://static.igem.org/mediawiki/2016/5/5e/T--UrbanTundra_Edmonton--JulyNotebook_.pdf" target="_blank">July</a></h3> |
| + | <h3 style:"text-align: center"><a href="https://static.igem.org/mediawiki/2016/5/5d/T--UrbanTundra_Edmonton--AugustNotebook_.pdf" target="_blank">August</a></h3> |
| + | <h3 style:"text-align: center"><a href="https://static.igem.org/mediawiki/2016/e/eb/T--UrbanTundra_Edmonton--SeptemberNotebook_.pdf" target="_blank">September</a></h3> |
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