Difference between revisions of "Team:NUDT CHINA/Part Collection"

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<h1>
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<br/><br/><br/>NUDT_CHINA: This page is under construction.<br/><br/><br/>
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<span><span style="color:#ff1015">PAGE STILL UNDER CONSTRUCTION</span></span>
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</h1>
  
  
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<h1>
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<span><span style="color:#7f1015">PPI Toolkit</span></span>
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</h1>
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<h2>
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<span><span style="color:#7f1015">A Novel Toolkit for protein-protein interaction (PPI) study</span></span><hr />
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</h2>
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Parts included in our collection</strong></span>
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</p>
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">BBa_K1997000, BBa_K1997001, BBa_K1997002, BBa_K1997003,
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BBa_K1997004, BBa_K1997005, BBa_K1997012, BBa_K1997013, BBa_K1997014, BBa_K1997015,
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BBa_K1997016, BBa_K1997017, BBa_K1997018, BBa_K1997019, BBa_K1997020, BBa_K1997021,
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BBa_K1997022, BBa_K1997023</span>
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</p>
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">&nbsp;</span>
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</p>
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<h3>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Introduction</span>
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</h3>
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<div style="text-indent:22pt;">
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit,
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contained 18 new parts design for protein-protein interaction (PPI) researches. </span>
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</p>
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">In PPI Toolkit, we collected basic parts
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containing the coding sequence of three split-reporting systems: split-GFP,
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split-Luciferase, and split-HRP. All those basic parts were codon-optimized to
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be expressed in </span><i><span style="line-height:2;font-family:Perpetua;font-size:18px;">E.coli</span></i><span style="line-height:2;font-family:Perpetua;font-size:18px;">, and
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sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining
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split-reporting system with a novel substitution system were designed,
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characterized and then added into the collection. Through which two proteins
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could be fused with their corresponding split-reporter fragment at the same
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time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning
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process.</span>
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</p>
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<p>
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional
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validated, well characterized and submitted to the registry.</span>
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</p>
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</div>
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</br>
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</br></br>
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</br>
  
  

Revision as of 04:20, 15 October 2016

NUDT_CHINA 2016

PAGE STILL UNDER CONSTRUCTION

PPI Toolkit

A Novel Toolkit for protein-protein interaction (PPI) study

Parts included in our collection

BBa_K1997000, BBa_K1997001, BBa_K1997002, BBa_K1997003, BBa_K1997004, BBa_K1997005, BBa_K1997012, BBa_K1997013, BBa_K1997014, BBa_K1997015, BBa_K1997016, BBa_K1997017, BBa_K1997018, BBa_K1997019, BBa_K1997020, BBa_K1997021, BBa_K1997022, BBa_K1997023

 

Introduction

This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.

In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.

All parts in this kit were functional validated, well characterized and submitted to the registry.






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