Difference between revisions of "Team:ZJU-China/Proof"

    we did co-transformation for the red light and green light system separately. the bacteria after co-transformation were irradiated by red or green light in our device, and we use sfGFP as reporter gene. By monitoring relative intensity of fluorescence, we could prove the system’s feasibility. Following is the result observed by multiple plate reader.



    In addition, to realize the second generation of cipher machine, we need to ensure that the light-induced system could be coupled with the oscillation system. That is to say, the response time of the light-induced system must be shorter than half period of the oscillation. Thus, we measured the system’s response time. Following is the results:



    Conclusion: After a series of experiment, we are able to prove the feasibility of the light-induced system. While there is a possibility of leak, the differences between the two groups can be measured by devices. Also, after our measurement of the response time about 3 hours, it falls within the oscillation’s regulating range, which is consistent with our modeling results. So far, we can prove that the concept of the second generation of cipher machine is correct.

    After a discussion about our literature, we settled the size of each trap and the length, width, and height are 100 um, 85um and 5um, respectively, which is the most favorable one for oscillation experiment and dynamic response model. Two traps stand 150um apart at the same side, while for the opposite side, the groove is 200um wide and 100um deep which is convenient for fluid to pass. Also, according to our calculation, we are quite sure that this design can increase the flow of AHL quorum sensing substance, and can let the cells at the edge of expansion colonies be watered away, making it possible for bacteria in the traps maintaining a continuous exponential growth.For observing convenience, we divide the traps into 4 groups, and each has 30 traps. The distance between two groups is sixty thousands um.

    For overall experiment, we also designed a device to help us using the microfludic chip. As for the body part, the upper platform has four through-holes, for putting in the LEDs. The lower part has the two slots, size of each slot are the same as the microfluidic chip. And we have a shading baffle whose size is appropriate with slots. Devices and shading baffle are produced by 3D printing, the material we chose is resin. It has smooth surface, high precision, and good hardness. Taking into account that the device will be placed in the thermostat, we used a kind of resin that has small thermal deformation coefficient, this will ensure that assembling won’t be a problem. In order to ensure that the experiment will not be interfered by the stray light, all surfaces are sprayed with black matte paint.