Transformation

Requirements for each sample:

•   1 tube of thawed chemically competent cells (keep covered in ice at all times).
•   1-10 uL ligation product cloned in plasmid.
•   250 uL SOC
•  1 LB plate with appropriate antibiotics

Procedure:

•   Add DNA to cells
•   Incubate on ice for 0-20 minutes
•   Heat shock at 42 °C for 30 seconds
•  Incubate on ice for 0-2 minutes
•   Add SOC
•  Incubate on rotator in 37 °C incubator for 0-60 minutes
•  Plate 50-250 uL