Difference between revisions of "Team:Stanford-Brown/SB16 BioMembrane Latex"
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| − | <div class="col-sm-12 pagetext" | + | <div class="col-sm-12 pagetext">In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development. |
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| − | <h1 class="sectionTitle-R"> | + | <h1 class="sectionTitle-R">Genetic Constructs</h1> |
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Revision as of 18:44, 9 October 2016
Experimental Design
INSERT INTRO HERE
In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development.
Genetic Constructs
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