Stanford-Brown 2016



We are very grateful for financial support from Stanford University REU, Stanford VPUE Grant for Undergraduate Research, Brown University UTRA, NASA Space Technology Mission Directorate Early Stage Innovation, and the Rhode Island Space Grant Consortium.


Team Member Contributions

All experiments were conducted by the team members and all images are originally created unless otherwise noted. List of sub-project attributions:
  • Collagen/Elastin: Charles Gleason, Anna Le, Eric Liu
  • p-Aramid: Eric Liu, Anna Le, Charles Gleason
  • Latex: Gordon Sun, Taylor Sihavong, Elias Robinson
  • Nylon: Charles Gleason, Anna Le, Eric Liu
  • UV Protection: Elias Robinson, Michael Becich
  • Gas Production: Taylor Sihavong
  • Chromoproteins: Cynthia Hale-Philips, Theresa Sievert, Taylor Pullinger
  • Fluorophore-Quencher: Michael Becich
  • IRES: Julia Gross, Amy Weissenbach
  • Aptamer Purification: Amy Weissenbach, Julia Gross, Michael Becich
  • Wiki Architect: Taylor Pullinger (with support from former team member, Forrest Tran, Stanford-Brown iGEM 2015)
  • Graphic Design: Taylor Sihavong, Taylor Pullinger
  • Human Practices Liason: Amy Weissenbach
  • Modeling/Software: Gordon Sun, Eric Liu, Michael Becich

Project Support and Advice

Thank you to our advisors! Especially Trevor! who was always there for us even on those late nights.

Functional Nucleic Acid Advice
Mark Ditzler, NASA Research Scientist
Christina Smolke, Stanford University Associate Professor of Bioengineering

Melanin Production Metabolic Engineering
Guillermo Gosset, Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México

Protein Extraction Advice
Jesica Navarrete

Microscopy Instruction
Ivan Paulino-Lima

Nils J. H. Averesch

General Lab Instruction/Troubleshooting
Trevor! Kalkus
Griffin McCutcheon
Ryan Kent
Kosuke Fujishima
Jesica Navarrete
Kara Rogers

We appreciate all the people involved in helping make a successful iGEM team!
We would like to thank Dr. Lynn Rothschild, Dr. Kara Rogers, Dr. Gary Wessel, Dr. Kosuke Fujishima, Dr. Ivan Paulino-Lima, Dr. Mark Ditzler, Griffin McCutcheon, Trevor! Kalkus (Stanford-Brown iGEM 2013), Ryan Kent (Brown-Stanford iGEM 2011), Jesica Navarrete, Simon Vecchioni (Stanford-Brown iGEM 2013), Jim Head, Jill Tartar, Margaret Race, and Maria Chavez.

Company Sponsors

DNA 2.0

Team Training and Project Start

Stanford University is well equipped for synthetic biology work, as an undergraduate Bioengineering program with several lab courses exists in full force. Brown University offers a Synthetic Biology Course every other year taught by Gary Wessel. We alternated between using Dr. Lynn Rothschild's lab at the National Aeronautics and Space Administration and the open-resource Uytengsu Teaching Center in Stanford's Shriram Center for Bioengineering and Chemical Engineering. Most of the members on the Stanford side of the team became acquainted with bioengineering lab work through BIOE44 (Fundamentals for Engineering Biology Lab). This course is generally taken as a sophomore, and some of our team members took it during the fall quarter of 2015-2016 (9/21/2015-12/11/2015). Materials for this course, such as the syllabus, are readily available online: here is an example from 2014.

Our team had brainstorming meetings and cross-continental team Skype sessions beginning in March 2016, where we stitched together rough subprojects and the overall theme of bioballoon. Our work in the lab did not start until the beginning of June 2016, when the Brown students ended their spring semester and travelled to California to work with the Stanford side in person. Stanford students got to lab after finishing their spring quarter in late June. All projects were begun by the Brown students, and ended by the Stanford students during the regular school year as the Jamboree approached.