Difference between revisions of "Team:Stanford-Brown/SB16 BioMembrane Latex"

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<div class="col-sm-12 pagetext">In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development. 
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<h1 class="sectionTitle-R">Genetic Constructs</h1>
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<h1 class="sectionTitle-R">Experimental Design</h1>
 
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<div class="col-sm-7 pagetext-R"><div class="text">Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. </div>
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<div class="col-sm-7 pagetext-R"><div class="text">In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development.   </div>
 
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Revision as of 18:47, 9 October 2016


Stanford-Brown 2016

Experimental Design

INSERT INTRO HERE

Experimental Design

In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development.
Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here. Stanford-Brown iGEMmers paste your contributions here.