Difference between revisions of "Team:Stanford-Brown/SB16 BioMembrane Latex"
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Revision as of 19:36, 9 October 2016
Introduction
INSERT INTRO HERE
Experimental Design
In order to appropriate enhanced IPP and subsequently latex precursor production, we took advantage of E. coli's endogenous MEP pathway, and implemented an HMG-CoA reductase pathway for enhanced IPP production. Both pathways result in the production of IPP and its isomer, DMAPP. However, the HMG-CoA pathway is exogenous to E. coli and was needed for enhanced production of IPP. While the MEP pathway converts pyruvate and G3P into IPP/DMAPP through a seven step process, the HMG-CoA (MVA) pathway converts acetyl CoA into IPP/DMAPP through a six step process. Both pathways employ the use of metabolic products produced and used by glycolysis and cellular respiration (calvin cycle); hence enhanced expression of these pathways is expected to affect cellular growth and development.
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