Difference between revisions of "Team:NUDT CHINA/HP/Silver"

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     <meta name="HandheldFriendly" content="True" />
 
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     <meta name="MobileOptimized" content="320" />
 
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    <meta name="viewport" content="width=device-width, initial-scale=1.0" />
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  <meta name="viewport" content="width=device-width, initial-scale=1.0",minimum-scale=0.2, maximum-scale=2.0, user-scalable=yes" />
 
     <meta content="on" http-equiv="cleartype" />
 
     <meta content="on" http-equiv="cleartype" />
 
     <meta property="og:title" content="Home" />
 
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       <div class="inner-wrap">
 
       <div class="inner-wrap">
 
         <!-- Off Canvas Menu -->
 
         <!-- Off Canvas Menu -->
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        <aside class="right-off-canvas-menu" aria-hidden="true">
 
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          <a role="search" class="mobile-search" title="Search" href="/search/" data-search-modal>Search</a>
 
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          <a class="button button-table large" title="Tickets" href="https#/tickets/">
 
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            <span class="first-icon-cell">
 
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              <i class="icon icon-ticket"></i>
 
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            </span>
 
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            <span class="text-cell">Tickets</span>
 
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              <i class="icon icon-anglebracket-right"></i>
 
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          <div data-cart-open-container style="margin-left: 0; width: 0;">
 
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            <a id="my-visit-opener-mobile" class="button button-table large" data-cart-open style="display: none;">
 
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                <i class="icon icon-add-to-visit"></i>
 
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              <span class="text-cell">My&nbsp;Visit</span>
 
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                <i class="icon icon-anglebracket-right"></i>
 
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              </span>
 
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            </a>
 
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          </div>
 
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          <ul class="mobile-nav-global">
 
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            <li>
 
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              <a class="nav-global__link" title="" href="/explore/">Explore</a></li>
 
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            <li>
 
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              <a class="nav-global__link" title="" href="/visit/">Visit-Cls</a></li>
 
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            <li>
 
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              <a class="nav-global__link" title="" href="/support/">Support</a></li>
 
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          </ul>
 
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          <ul class="nav-utility">
 
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            <li>
 
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              <a class="nav-utility__link" title="Members" href="/support/membership/">Members</a></li>
 
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            <li>
 
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              <a class="nav-utility__link" title="Educators" href="/education/">Educators</a></li>
 
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          </ul>
 
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         <!-- close the off-canvas menu -->
 
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         <a class="exit-off-canvas"></a>
 
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                     <a class="nav-global__link" title="" href="#">AWARDS</a></li>
 
                     <a class="nav-global__link" title="" href="#">AWARDS</a></li>
 
                 </ul>
 
                 </ul>
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<style>#menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;}  
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<style>
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.content{
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box-shadow: 6px 6px 3px #888888;
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}
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#menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;}  
 
#menu01 .arrow-up{margin-right:6.429rem;}
 
#menu01 .arrow-up{margin-right:6.429rem;}
 
#menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;}
 
#menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;}
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                 </div>
 
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</nav>
 
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               <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } }</style>
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               <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } }
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#feature{
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<div class="breadcrumb-share-wrap">
 
<div class="breadcrumb-share-wrap">
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         <div id="feature" class="row collapse text-light feature-footer-active" style="background-color: rgb(3,13,38);background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-position:0px -350px;background-repeat:no-repeat;">
 
           <div class="inner-wrap medium-24 columns">
 
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             <!--TYPO3SEARCH_begin-->
 
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               <div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide">
 
               <div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide">
 
                 <div class="slide first">
 
                 <div class="slide first">
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                   <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS3?action=raw&ctype=text/css" />
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                   <link rel="stylesheet" href="/Team--NUDT_CHINA/CSS3.css" />
 
                   <!-- Quick initialize with theme color from first slide -->
 
                   <!-- Quick initialize with theme color from first slide -->
 
                   <div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601">
 
                   <div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601">
 
                     <div class="columns">
 
                     <div class="columns">
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                       <section class="big-header bh__height-size-3 text-light bh__video">
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                       <section class="big-header bh__height-size-3 text-light bh__video" style="height:300px;">
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                         <div class="bh__background bh__background-color " style="background-color: rgb(3,13,38);background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-size:100%;background-repeat:no-repeat;"></div>
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                         <div class="bh__background bh__background-color "></div>
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                         <div class="bh__content-wrap">
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                         <div class="bh__content-wrap" style="height:120px;">
 
                           <div class="bh__content ">
 
                           <div class="bh__content ">
 
                             <div class="bh__content-inner-wrap">
 
                             <div class="bh__content-inner-wrap">
 
                               <!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3-->
 
                               <!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3-->
−
                              <h1 class="bh__title ">
 
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                                <!--<a href="#">-->
 
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                                  <span class="line-wrap">
 
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                                    <span class="line">Cancer,</span></span>
 
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                                  <br>
 
−
                                  <span style="line-height:75px;" class="line-wrap">
 
−
                                    <span style="line-height:75px;" class="line">No More Hiding</span></span>
 
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                                <!--</a>-->
 
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                              </h1>
 
 
                               <h2 style="font-size:36px;" class="bh__title ">
 
                               <h2 style="font-size:36px;" class="bh__title ">
 
                                 <!--<a href="#">-->
 
                                 <!--<a href="#">-->
−
                                  <span style="line-height:41px;" class="line-wrap">
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                                <span style="line-height:46px;" class="line-wrap">
−
                                    <span style="line-height:41px;" class="line">Development of A Novel</span></span>
+
                                  <span style="line-height:46px;" class="line">Development of A Novel</span></span>
−
                                  <span style="line-height:41px;" class="line-wrap">
+
                                <span style="line-height:46px;" class="line-wrap">
−
                                    <span style="line-height:41px;" class="line">Blood-MicroRNA Handy Detection System with CRISPR</span></span>
+
                                  <span style="line-height:46px;" class="line">Blood-MicroRNA Handy Detection System with CRISPR</span></span>
−
                                 <!--</a>-->
+
                                 <!--</a>--></h2>
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                              </h2>
+
 
                             </div>
 
                             </div>
 
                           </div>
 
                           </div>
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               <div class="cycle-pager" id="cycle-pager-4811"></div>
 
               <div class="cycle-pager" id="cycle-pager-4811"></div>
 
             </div>
 
             </div>
−
            <!--TYPO3SEARCH_end--></div>
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          </div>
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          <!-- end .columns --></div>
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        </div>
 
         <!-- end #feature -->
 
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       <div style="margin-top:80px;" id="content-wrap-ink"><!-- adjust in add_JS -->
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       <div style="margin-top:20px;" id="content-wrap-ink"><!-- adjust in add_JS -->
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<div class="row footer-link" style="">
+
<div class="row footer-link" style="">
 +
<div style="text-align:right;"><h5>
 +
<a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA">HomePage</a> &bull;
 +
<!-- 修改这里！！ -->Human Practice &bull;
 +
<a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA/Description"><!-- 修改这里！！ -->Silver</a>
 +
</h5><hr style="width:40%;margin-left:60%;border-top:1px solid rgb(10,31,84);" /></div>
 +
 
 +
 
 +
 
 +
 
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 +
<!--在这里插入内容-->
 +
 
 +
<h2>
 +
<span><span style="color:#7f1015">Overview</span></span><hr />
 +
</h2>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In our project this year, we designed a novel cell-free platform built with synthetic bio-components to achieve the low-cost, handy and visualized detection of serum miRNAs, which can be employed in low-resource settings. The idea that we chose this topic was to serve the world with a novel early cancer detection system that could help more people, especially in remote areas to take early screening and detection of cancers.</span>
 +
</p>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">However, to help with the fight against cancer, developing new technologies and applications is not enough. Thus We distributed our Human Practice work in three parts based on our project.</span>
 +
</p>
 +
<p style="text-align:center;">
 +
<table class="MsoTableGrid" style="width:426.1pt;">
 +
<tbody>
 +
<tr>
 +
<td width="331" valign="top" style="border:1.0000pt solid #4BACC6;background:#4BACC6;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<b><span style="font-family:Arial;color:#FFFFFF;font-size:18px;">HP Modules</span></b><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
<td width="520" valign="top" colspan="3" style="border:1.0000pt solid #4BACC6;background:#4BACC6;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<b><span style="font-family:Arial;color:#FFFFFF;font-size:18px;">Activities</span></b><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="331" valign="top" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<b><span style="font-family:Arial;font-size:10.5pt;">Collaborations with Hospital &amp; Health Center</span></b><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
<td width="183" valign="top" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Interviews with Doctors</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
<td width="183" valign="top" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Survey in the Hospital </span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
<td width="154" valign="top" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">sample collecting</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="331" valign="center" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="text-align:center;">
 +
<b><span style="font-family:Arial;font-size:11pt;">Product Evaluation</span></b><span style="font-family:宋体;font-size:12pt;"></span>
 +
</p>
 +
</td>
 +
<td width="183" valign="center" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="margin-left:0.0000pt;text-indent:0.0000pt;text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Collecting information around product</span><span style="font-family:宋体;color:#C00000;font-size:11.0000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="337" valign="center" colspan="2" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Evaluating Potentials and Costs of Our Product</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="331" valign="center" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="text-align:center;">
 +
<b><span style="font-family:Arial;font-size:11pt;">Raising awareness</span></b><b><span style="font-family:宋体;font-size:11pt;"></span></b>
 +
</p>
 +
</td>
 +
<td width="520" valign="top" colspan="3" style="border:1.0000pt solid #4BACC6;background:#FFFFFF;">
 +
<p class="p" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Different activities for all ages to arouse the awareness of cancer &amp; early cancer detection and to publize our project to the world.&nbsp;</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</p>
 +
</br>
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">PART I</span></span>
 +
</h2>
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">COLLABORATIONS WITH HOSPITAL & HEALTH CENTER</span></span><hr />
 +
</h2>
 +
 
 +
</br>
 +
 
 +
 
 +
<h3>
 +
<span style="color:#7f1015">Interviews with Doctors</span>
 +
</h3></br>
 +
<h4>
 +
<span style="color:#7f1015">1.Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span>
 +
</h4>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We talked to Dr.Li on issue about procedure of current cancer detection,
 +
gaps Between clinical application and basic research, doubted stability of miRNA in blood, efficiency of miRNA detection and expected prospect of miRNA. This helped us have a detailed understanding of current miRNA application in clinical use, from this we started to increase our attention to the testing validness and long-term effections of our project.
 +
</span>
 +
</p>
 +
</br>
 +
<h4>
 +
<span style="color:#7f1015">2.Dr.Hou Tao, Oncology Department，Second Xiangya Affiliated Hospital of CSU</span>
 +
</h4></span>
 +
</h4>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In interviews with Dr.Hou, we mainly discussed on costs of current early cancer detection methods. During this we found that the costs of current early cancer detection methods were to some extent too high for people especially the poor to afford. And this methods needed big instruments to analysis samples. It is hard for small hospitals in remote areas to utilize such instruments,  thus the remote people may not have the chance to take early cancer detection unless they go to big cities, which may bring pessimism to their thoughts to take the early cancer detection. This acted as an alert of what direction we should go.</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We also talked with Dr.Hou about disadvantages of current early detection methods to get help to determine our final project scheme.</span>
 +
</p>
 +
</br></br></br>
 +
 
 +
<h3>
 +
<span style="color:#7f1015">Survey In The Hospital & Health Center</span>
 +
</h3></br>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We did surveys in Second Xiangya Hospital affiliated to CSU and Health Center of NUDT about details of current early cancer detection methods, which acted as a reference to our project. </span>
 +
</p>
 +
<p>
 +
<table class="MsoTableGrid" align="center" style="width:526.95pt;">
 +
<tbody>
 +
<tr>
 +
<td width="123" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">METHOD</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="118" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">COST</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">(RMB)</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="150" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">TIME</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="199" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">ADVANTAGE</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="223" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">DISADVANTAGE</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="239" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">POPULARITY</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="123" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">PET-CT</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
</td>
 +
<td width="118" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">7000-8000</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="150" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">2-3h</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="199" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Detection for early and advanced tumor</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">No pain</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Valid</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="justify" style="text-align:justify;">
 +
<span style="font-family:Arial;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
</td>
 +
<td width="223" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Expensive</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Rare</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Radiation</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Condition requirements</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">(not fit for remote </span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">&nbsp;areas)</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#0000FF;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
</td>
 +
<td width="239" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">270</span><i><span style="font-family:Arial;font-size:10.5pt;">&nbsp;</span></i><span style="font-family:Arial;font-size:10.5pt;">in the whole country</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:8pt;">(data source:</span><i><span style="font-family:Arial;font-size:8pt;">2011-2015 National PET-CT Plan </span></i><span style="font-family:Arial;font-size:8pt;">by Chinese Department of Health)</span><span style="font-family:宋体;color:#0000FF;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="123" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">CT</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="118" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">200-500</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="150" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">20min</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="199" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Most effective for tumors in nervous system, head, chest and abdomen</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="223" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Only functions on a certain part</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Radiation</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Condition restriction</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
</td>
 +
<td width="239" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Rare in counties and remote areas</span><span style="font-family:宋体;color:#0000FF;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="123" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Tumor biomarker test</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="118" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">500</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:8pt;">(including 12 biomarkers)</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="150" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">10min</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:8pt;">(sample gathering)</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="199" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Easy to operate</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">No side effects</span><span style="font-family:宋体;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="223" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Requirements for instruments</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Low validness</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">Narrow testing range</span><span style="font-family:宋体;font-size:10.5pt;"></span>
 +
</p>
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
</td>
 +
<td width="239" valign="center" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;font-size:10.5pt;">City or central hospital</span><span style="font-family:宋体;color:#333333;font-size:7pt;background:#FFFFFF;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<span style="font-family:Arial;"></span>
 +
</p>
 +
 
 +
</p>
 +
</br>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for.
 +
</span>
 +
</p>
 +
 
 +
</br></br></br>
 +
<h3>
 +
<span style="color:#7f1015">Sample Collection for Wet Lab</span>
 +
</h3></br>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Since our project this year was to develop a blood-microRNA detection system as a novel method for early cancer detection, and results of previous wet lab all confirmed the effect of our detection system, it was necessary to apply it to serum as a final confirmation. We came to the health exam center of Affiliated Hospital of NUDT and talked with doctors and 5 non-small cell lung cancer(NSCLC) patients about our need for serum samples to and got their support and permission. Also we had 30 healthy postgraduates from NUDT as our volunteer of serum samples. And we collected the blood samples with the help of doctors.</span>
 +
</p>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Approve of the Informed Consent Form</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We submitted the Informed Consent Form to the department of ethics in NUDT. After days of checking it was approved to use.</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Consultation with doctors</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we found Dr.Tan, one of the principles in the department of clinical lab of the health center. He introduced the formal process of collecting blood samples to us and approved our Informed Consent Form. Dr.Tan also helped us with the collecting work.</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">3.Meeting with sample groups</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We talked to 5 cancer patients in different stages of NSCLC as well as 30 healthy volunteers. We introduced what we had been working on and explained what we would do with their blood samples. We also made a security guarantee that we would keep their personal information as secret.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">4.Processes of sample gathering </span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We gathered serum samples successfully with the help of doctors and nurses. And transported them to laboratory, stored in -80℃.</span>
 +
</p>
 +
</br>
 +
</br>
 +
</br>
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">PART II</span></span>
 +
</h2>
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">PRODUCT EVALUATION</span></span><hr />
 +
 
 +
<h3>
 +
<span style="color:#7f1015">Market Demands</span>
 +
</h3>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Previously we did surveys in hospitals and health center about current early cancer detection methods. To gather more information, we searched the Internet about the same issue. Here is the summary.</span>
 +
</p>
 +
 
 +
</html>
 +
[[File:biaoge_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span>
 +
</p>
 +
</br>
 +
<h3>
 +
<span style="color:#7f1015">Cost Evaluation</span>
 +
</h3>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We made an evaluation of efficiency and price of our detection system in this part to see how much our product may decrease the cost of biomarker detection for cancer, or to say how much our project could help the world with.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The other reason we made this cost evaluation was to make it a feedback for the project and find other aspects it may develop itself in.</span>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The following is the evaluation of our expected product cost.</span>
 +
</p>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">1.Cost Evaluation</span>
 +
</p>
 +
</br>
 +
<p>
 +
<table class="MsoTableGrid" style="width:426.1pt;">
 +
<tbody>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">name of reagents</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Unit price</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Use level</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Equivalent</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Ribonuclease inhibitor</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$32.2/200ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">37.6ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.006</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">phi29 DNA polymerase</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$70.5/250U(10U/ul)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">0.6ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$1.692</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Buffer phi29</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$19.5/5ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">3ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.012</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">dNTP</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$12.0/1ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">7.2ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.086</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Special probe</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.16</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">one</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.163</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">Cas9 protein</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$22.3/g</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">1ul(0.2uM)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.713</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="230" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">double distilled water</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="195" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$3.0/25kg</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">491.2ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="213" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal" align="center" style="text-align:center;">
 +
<span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.000</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Assuming that the protein can be made to the level of industrial production, the total reagent cost is about $2.672.</span>
 +
</p>
 +
<p>
 +
<table class="MsoTableGrid" style="width:426.1pt;">
 +
<tbody>
 +
<tr>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Name of equipment</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Business</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Price</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">centrifugal machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">TGL-16 high speed centrifuge (1.5ml*12 hole)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$77.979</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Freeze dryer</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">LY-15AC high efficiency freezing and drying machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$450.224</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">&nbsp;</span>
 +
</p>
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Constant temperature box</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">DH-250AB electric heating constant temperature incubator</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$335.908</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">HP printers</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">HP color laser integrated machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
<td width="284" valign="top" style="border:1.0000pt solid windowtext;">
 +
<p class="MsoNormal">
 +
<span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$427.382</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<span style="font-family:Arial;"></span>
 +
</p>
 +
 
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">All of these machines can be used for long and we don’t need to take them while testing miRNA outside. So we consider loss only. Centrifugal machine is used to extract serum while freeze dryer is used to freeze-dry relative reagents in the test paper. We apply HP color laser integrated machine to make colorimetric card.Briefly,the loss price is about $0.3.</span>
 +
</p>
 +
</br>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">2.Interpretation of Result</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)The total price of our project is around $3.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">During the experiment, we found that this method can achieve requirements of high sensitivity, low cost and easy operation.</span>
 +
</p>
 +
</br>
 +
 
 +
 
 +
 
 +
 
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">PART III</span></span>
 +
</h2>
 +
<h2 align="center"
 +
>
 +
<span><span style="color:#7f1015">RAISING AWARENESS</span></span><hr />
 +
 
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In the project this year, the aim of our creation of the product was to detect blood-microRNA thus to promote early cancer detection and then to help the world. </span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">However, to realize helping the world, this is far from enough. Thus we scheduled a series of Human Practice works for all ages to expand our project and ideas out of the lab---we raised people’s awareness of cancer&early cancer detection.</span>
 +
</p>
 +
</br>
 +
<h3>
 +
<span style="color:#7f1015">Internet Survey For The Public</span>
 +
</h3>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In this survey about cancer and early cancer detection, we we chose netizens as our respondent, thus the results would be more objective and random. And we may get more comprehensive and true answers. We designed an Internet survey and put it on social media. Until we stopped the Internet survey, there were 524 netizens who took part in it.</span>
 +
</p>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The survey mainly involved the three aspects:</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">(1)How much the public knew about early cancer detection.</span>
 +
</p>
 +
 
 +
</html>
 +
[[File:diaocha1_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">(2)What their main problems were when seeking for early cancer detection.</span>
 +
</p>
 +
</br>
 +
 
 +
</html>
 +
[[File:图片2_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">(3)To publize knowledge of cancer,early cancer detection and our project</span>
 +
</p>
 +
 
 +
 
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">a. What do you think of our project?</span>
 +
</p>
 +
</html>
 +
[[File:diaocha3_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">b. What do you think is acceptable of our project?</span>
 +
</p>
 +
</html>
 +
[[File:diaocha4_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">c.Do you expect to see our product put in to market?</span>
 +
</p>
 +
</html>
 +
[[File:diaocha5 NUDT CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The data from this survey reflected that although most people attached great importance to early cancer detection, not so many of them knew much about cancer detection, which reminded us that if we wanted to help the world with our handy tumor biomarkers detection system, lateral publicity of early cancer detection to arouse people’s awareness would play a vital role. </span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">What’s more, people had the idea to take early cancer detection, but were stopped by the high cost, possible radiation and operating complexity of current methods. Thus our handy, low-cost detection system may have great hope to decrease the pressure of high cost and complex steps of current early cancer detection methods.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We also learned that people held great expectations towards our project. However, validness of our product were what they doubted most, which was also a common problem of almost all biomarker tests. This reminded us that we must try to raise the validness of the product in our lataral work.</span>
 +
</p>
 +
</br>
 +
 
 +
 
 +
<h3>
 +
<span style="color:#7f1015">Ethics</span>
 +
</h3>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Since the idea this year has a close relationship with cancer early detection, we had a discussion standing in shoes of cancer patients.
 +
(click to view details of our discussion)</span>
 +
</p>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We held this discussion in the laboratory, and called on several students from different majors(eg. Chemistry, Medicine, Electronic Engineering) to take part. As we talked gradually deeper, our moods became increasingly low. Cancer has been a monster for so many years, but the ethical issues of treatment, finance, lie or tell and social assistance have never had an answer.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">This year what we want to spread is not only a novel early cancer detection system, but also awareness of people with cancer. We are all weak in the face of diseases, especially deadly ones like cancer. Only if we pay attention to our health and stay alert for accidental diseases can we live a healthy life for long.</span>
 +
</p>
 +
</br>
 +
<h3>
 +
<span style="color:#7f1015">Grandpas and Grannys’ Mini Lecture </span>
 +
</h3>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We planned mini lectures of knowledge of cancer and early cancer detection in several districts mainly for the elder to increase their attention to early cancer detection. Also we wanted to spread the idea that early cancer was not terrifying and could be cured or prevented. Finally , we publicized knowledge about synthetic biology, iGEM, and our project this year.</span>
 +
</p>
 +
</br>
 +
 
 +
</html>
 +
[[File:社区1_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
</br></br>
 +
</html>
 +
[[File:社区2_NUDT_CHINA.jpg|700px|center]]
 +
<html></br></br>
 +
</html>
 +
[[File:社区3_NUDT_CHINA.jpg|700px|center]]
 +
<html></br></br>
 +
</html>
 +
[[File:社区4_NUDT_CHINA.jpg|700px|center]]
 +
<html></br></br>
 +
 
 +
 
 +
<h3>
 +
<span style="color:#7f1015">Lab day for high school students </span>
 +
</h3>
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">We invited several high school students who were enthusiastic about biology and their teachers from Changsha No.1 High School to our laboratory.
 +
As for the activities, firstly we made a presentation about synthetic biology, iGEM and our project. Then we showed these guests around our lab and introduced our instruments and experiments, and guided them to carry out some interesting biology experiments including polymerase digest and electrophoresis.</span>
 +
</p>
 +
</br>
 +
</html>
 +
[[File:实验室1_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
</br></br>
 +
</html>
 +
[[File:实验室2_NUDT_CHINA.jpg|700px|center]]
 +
<html></br></br>
 +
</html>
 +
[[File:实验室3_NUDT_CHINA.jpg|700px|center]]
 +
<html></br>
 +
</br>
 +
<h3>
 +
<span style="color:#7f1015">Fun game with our topic</span>
 +
</h3>
 +
 
 +
 
 +
 
 +
</br></br></br></br></br></br></br></br></br></br>
 +
</br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">Adventure of iGEM is a third person adventure game that aims at introducing iGEM and our project this year to everyone in an interesting way.</span>
 +
</p>
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">A normal student named Itazk joins the team of iGEM. One day he falls asleep in the lab and has a dream. In this dream, he must work out some puzzles to find artifacts of NUDT’s blood-microRNA detection system and defeat Monster Cancer.</span>
 +
</p>
 +
</br>
 +
<h3>
 +
<span style="color:#7f1015">Long-term sustainability</span>
 +
</h3>
 +
 
 +
 
 +
 
 +
 
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">To help our project leave a deep impression on people, also to expand the range of our publicity, we made useful little things with knowledge of cancer/early cancer detection/iGEM for families as souveniors.</span>
 +
</p>
 +
</html>
 +
[[File:扇子图案_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:16px;">mini poster for everyone</span>
 +
</p>
 +
</br></br></br>
 +
</html>
 +
[[File:发扇子_NUDT_CHINA.jpg|700px|center]]
 +
<html>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;"></span>
 +
</p>
 +
</br>
 +
 
 +
 
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<br/><br/><br/>NUDT_CHINA: This page is under construction.<br/><br/><br/>
 
  
  
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+
} #c_menu9909 .medium-6{ text-align:center; width:auto; border:solid 0px red; } #c_menu9909 .medium-6 h5{color:white;}#c_menu9909 .medium-6{height:315px;border:solid 0px red;}</style>
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      <!--div class="medium-6 columns" style="width:auto;">
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+
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                    <a href="/Team:NUDT_CHINA/Team">Team</a></li>
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                    <a href="/Team:NUDT_CHINA/Results">Results</a></li>
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                    <a href="/Team:NUDT_CHINA/Notebook">Notebook</a></li>
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             </div>
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               <h5 class="column-header">PARTS</h5>
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                    <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a></li>
+
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+
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+
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              <br/>
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  <A href="/Team:NUDT_CHINA/Safety" style="text-decoration:none;"><h5 class="column-header">SAFETY</h5></A>
+
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+
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  <br/>
+
−
  <br/>
+
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  <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 class="column-header">ATTRIBUTIONS</h5></A>
+
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+
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            </div-->
+
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              <h5 class="column-header">HUMAN
+
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                <BR/>PRACTICES</h5>
+
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+
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+
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                    <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a></li>
+
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+
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                    <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a></li>
+
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+
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                    <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a></li>
+
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                  <li>
+
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                    <a href="/Team:NUDT_CHINA/Engagement">Engagement</a></li>
+
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                </ul>
+
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+
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            </div>
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+
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              <h5 class="column-header">AWARDS</h5>
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+
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                </ul>
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+
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       </div>
 
       </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">PROJECT</h5>
 +
        <div class="menu-link-container">
 +
         
 +
           
 +
              <a href="/Team:NUDT_CHINA/Description">Description</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Design">Design</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Experiments">Experiments</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Proof">Proof of Concept</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Results">Results</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Notebook">Notebook</a><br/>
 +
         
 +
        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">PARTS</h5>
 +
        <div class="menu-link-container">
 +
         
 +
           
 +
              <a href="/Team:NUDT_CHINA/Parts">Parts</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a><br/>
 +
         
 +
        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <a href="/Team:NUDT_CHINA/Safety" style="text-decoration:none;">
 +
          <h5 class="column-header">SAFETY</h5></a>
 +
       
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <a href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;">
 +
          <h5 class="column-header">ATTRIBUTIONS</h5></a>
 +
       
 +
      </div>  
 +
      <!--div class="medium-6 columns" style="width:auto;">
 +
      <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 class="column-header">ATTRIBUTIONS</h5></A>
 +
      <div class="menu-link-container"></div></div-->
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">HUMAN
 +
          <br>PRACTICES</h5>
 +
        <div class="menu-link-container">
 +
         
 +
           
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              <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Engagement">Engagement</a><br/>
 +
         
 +
        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">AWARDS</h5>
 +
        <div class="menu-link-container">
 +
         
 +
           
 +
              <a href="/Team:NUDT_CHINA/Model">Model</a><br/>
 +
         
 +
        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:0px;height:0px;"></div>
 +
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 +
  </div>
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</div>  
 
       <footer id="footer">
 
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<style>
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#to_top{
 +
position:fixed;
 +
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 +
right:0px;
 +
width:60px;
 +
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 +
font-size:14px;
 +
line-height:60px;
 +
text-align:center;
 +
background-color:black;
 +
color:rgb(246,168,0);
 +
cursor:pointer;
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}
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</style>
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<div onclick="top_it()" id="to_top">
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TOP
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<script type="text/javascript">
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var oTop = document.getElementById("to_top");
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oTop.style.display="none";
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var top_break=false;
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 +
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 +
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window.onscroll = function(){
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    var scrolltop = document.documentElement.scrollTop || document.body.scrollTop;
 +
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oTop.style.display="block";
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goTop();return false;
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function goTop(acceleration, time) {
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if(top_break){
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oTop.style.display="none";
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if (document.documentElement) {
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x1 = document.documentElement.scrollLeft || 0;
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y1 = document.documentElement.scrollTop || 0;
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}
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if (document.body) {
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x2 = document.body.scrollLeft || 0;
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y2 = document.body.scrollTop || 0;
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var x3 = window.scrollX || 0;
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var y3 = window.scrollY || 0;
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// 滚动条到页面顶部的水平距离
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Revision as of 08:13, 19 October 2016

NUDT_CHINA 2016

HomePage • Human Practice • Silver

Overview

In our project this year, we designed a novel cell-free platform built with synthetic bio-components to achieve the low-cost, handy and visualized detection of serum miRNAs, which can be employed in low-resource settings. The idea that we chose this topic was to serve the world with a novel early cancer detection system that could help more people, especially in remote areas to take early screening and detection of cancers.

However, to help with the fight against cancer, developing new technologies and applications is not enough. Thus We distributed our Human Practice work in three parts based on our project.

HP Modules

Activities

Collaborations with Hospital & Health Center

Interviews with Doctors

Survey in the Hospital

sample collecting

Product Evaluation

Collecting information around product

Evaluating Potentials and Costs of Our Product

Raising awareness

Different activities for all ages to arouse the awareness of cancer & early cancer detection and to publize our project to the world. 


PART I

COLLABORATIONS WITH HOSPITAL & HEALTH CENTER


Interviews with Doctors


1.Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU

We talked to Dr.Li on issue about procedure of current cancer detection, gaps Between clinical application and basic research, doubted stability of miRNA in blood, efficiency of miRNA detection and expected prospect of miRNA. This helped us have a detailed understanding of current miRNA application in clinical use, from this we started to increase our attention to the testing validness and long-term effections of our project.


2.Dr.Hou Tao, Oncology Department，Second Xiangya Affiliated Hospital of CSU

In interviews with Dr.Hou, we mainly discussed on costs of current early cancer detection methods. During this we found that the costs of current early cancer detection methods were to some extent too high for people especially the poor to afford. And this methods needed big instruments to analysis samples. It is hard for small hospitals in remote areas to utilize such instruments, thus the remote people may not have the chance to take early cancer detection unless they go to big cities, which may bring pessimism to their thoughts to take the early cancer detection. This acted as an alert of what direction we should go.

We also talked with Dr.Hou about disadvantages of current early detection methods to get help to determine our final project scheme.




Survey In The Hospital & Health Center


We did surveys in Second Xiangya Hospital affiliated to CSU and Health Center of NUDT about details of current early cancer detection methods, which acted as a reference to our project.

METHOD

COST

(RMB)

TIME

ADVANTAGE

DISADVANTAGE

POPULARITY

PET-CT

 

7000-8000

2-3h

Detection for early and advanced tumor

No pain

Valid

 

Expensive

Rare

Radiation

Condition requirements

(not fit for remote

 areas)

 

270 in the whole country

(data source:2011-2015 National PET-CT Plan by Chinese Department of Health)

CT

200-500

20min

Most effective for tumors in nervous system, head, chest and abdomen

Only functions on a certain part

Radiation

Condition restriction

 

Rare in counties and remote areas

Tumor biomarker test

500

(including 12 biomarkers)

10min

(sample gathering)

Easy to operate

No side effects

Requirements for instruments

Low validness

Narrow testing range

 

City or central hospital


From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for.




Sample Collection for Wet Lab


Since our project this year was to develop a blood-microRNA detection system as a novel method for early cancer detection, and results of previous wet lab all confirmed the effect of our detection system, it was necessary to apply it to serum as a final confirmation. We came to the health exam center of Affiliated Hospital of NUDT and talked with doctors and 5 non-small cell lung cancer(NSCLC) patients about our need for serum samples to and got their support and permission. Also we had 30 healthy postgraduates from NUDT as our volunteer of serum samples. And we collected the blood samples with the help of doctors.

1.Approve of the Informed Consent Form

We submitted the Informed Consent Form to the department of ethics in NUDT. After days of checking it was approved to use.

2. Consultation with doctors

Firstly we found Dr.Tan, one of the principles in the department of clinical lab of the health center. He introduced the formal process of collecting blood samples to us and approved our Informed Consent Form. Dr.Tan also helped us with the collecting work.

3.Meeting with sample groups

We talked to 5 cancer patients in different stages of NSCLC as well as 30 healthy volunteers. We introduced what we had been working on and explained what we would do with their blood samples. We also made a security guarantee that we would keep their personal information as secret.

4.Processes of sample gathering

We gathered serum samples successfully with the help of doctors and nurses. And transported them to laboratory, stored in -80℃.




PART II

PRODUCT EVALUATION

Market Demands

Previously we did surveys in hospitals and health center about current early cancer detection methods. To gather more information, we searched the Internet about the same issue. Here is the summary.

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Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.


Cost Evaluation

We made an evaluation of efficiency and price of our detection system in this part to see how much our product may decrease the cost of biomarker detection for cancer, or to say how much our project could help the world with.

The other reason we made this cost evaluation was to make it a feedback for the project and find other aspects it may develop itself in.

The following is the evaluation of our expected product cost.

1.Cost Evaluation


name of reagents

Unit price

Use level

Equivalent

Ribonuclease inhibitor

$32.2/200ml

37.6ul

$0.006

phi29 DNA polymerase

$70.5/250U(10U/ul)

0.6ul

$1.692

Buffer phi29

$19.5/5ml

3ul

$0.012

dNTP

$12.0/1ml

7.2ul

$0.086

Special probe

$0.16

one

$0.163

Cas9 protein

$22.3/g

1ul(0.2uM)

$0.713

double distilled water

$3.0/25kg

491.2ul

$0.000

Assuming that the protein can be made to the level of industrial production, the total reagent cost is about $2.672.

Name of equipment

Business

Price

centrifugal machine

TGL-16 high speed centrifuge (1.5ml*12 hole)

$77.979

Freeze dryer

LY-15AC high efficiency freezing and drying machine

$450.224

 

Constant temperature box

DH-250AB electric heating constant temperature incubator

$335.908

HP printers

HP color laser integrated machine

$427.382

All of these machines can be used for long and we don’t need to take them while testing miRNA outside. So we consider loss only. Centrifugal machine is used to extract serum while freeze dryer is used to freeze-dry relative reagents in the test paper. We apply HP color laser integrated machine to make colorimetric card.Briefly,the loss price is about $0.3.


2.Interpretation of Result

(1)The total price of our project is around $3.

During the experiment, we found that this method can achieve requirements of high sensitivity, low cost and easy operation.


PART III

RAISING AWARENESS

In the project this year, the aim of our creation of the product was to detect blood-microRNA thus to promote early cancer detection and then to help the world.

However, to realize helping the world, this is far from enough. Thus we scheduled a series of Human Practice works for all ages to expand our project and ideas out of the lab---we raised people’s awareness of cancer&early cancer detection.


Internet Survey For The Public

In this survey about cancer and early cancer detection, we we chose netizens as our respondent, thus the results would be more objective and random. And we may get more comprehensive and true answers. We designed an Internet survey and put it on social media. Until we stopped the Internet survey, there were 524 netizens who took part in it.

The survey mainly involved the three aspects:

(1)How much the public knew about early cancer detection.

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(2)What their main problems were when seeking for early cancer detection.


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(3)To publize knowledge of cancer,early cancer detection and our project

a. What do you think of our project?

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b. What do you think is acceptable of our project?

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c.Do you expect to see our product put in to market?

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The data from this survey reflected that although most people attached great importance to early cancer detection, not so many of them knew much about cancer detection, which reminded us that if we wanted to help the world with our handy tumor biomarkers detection system, lateral publicity of early cancer detection to arouse people’s awareness would play a vital role.

What’s more, people had the idea to take early cancer detection, but were stopped by the high cost, possible radiation and operating complexity of current methods. Thus our handy, low-cost detection system may have great hope to decrease the pressure of high cost and complex steps of current early cancer detection methods.

We also learned that people held great expectations towards our project. However, validness of our product were what they doubted most, which was also a common problem of almost all biomarker tests. This reminded us that we must try to raise the validness of the product in our lataral work.


Ethics

Since the idea this year has a close relationship with cancer early detection, we had a discussion standing in shoes of cancer patients. (click to view details of our discussion)

We held this discussion in the laboratory, and called on several students from different majors(eg. Chemistry, Medicine, Electronic Engineering) to take part. As we talked gradually deeper, our moods became increasingly low. Cancer has been a monster for so many years, but the ethical issues of treatment, finance, lie or tell and social assistance have never had an answer.

This year what we want to spread is not only a novel early cancer detection system, but also awareness of people with cancer. We are all weak in the face of diseases, especially deadly ones like cancer. Only if we pay attention to our health and stay alert for accidental diseases can we live a healthy life for long.


Grandpas and Grannys’ Mini Lecture

We planned mini lectures of knowledge of cancer and early cancer detection in several districts mainly for the elder to increase their attention to early cancer detection. Also we wanted to spread the idea that early cancer was not terrifying and could be cured or prevented. Finally , we publicized knowledge about synthetic biology, iGEM, and our project this year.


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Lab day for high school students

We invited several high school students who were enthusiastic about biology and their teachers from Changsha No.1 High School to our laboratory. As for the activities, firstly we made a presentation about synthetic biology, iGEM and our project. Then we showed these guests around our lab and introduced our instruments and experiments, and guided them to carry out some interesting biology experiments including polymerase digest and electrophoresis.


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Fun game with our topic












Adventure of iGEM is a third person adventure game that aims at introducing iGEM and our project this year to everyone in an interesting way.

A normal student named Itazk joins the team of iGEM. One day he falls asleep in the lab and has a dream. In this dream, he must work out some puzzles to find artifacts of NUDT’s blood-microRNA detection system and defeat Monster Cancer.


Long-term sustainability

To help our project leave a deep impression on people, also to expand the range of our publicity, we made useful little things with knowledge of cancer/early cancer detection/iGEM for families as souveniors.

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mini poster for everyone




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