Difference between revisions of "Team:NUDT CHINA/Part Collection"
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<span><span style="color:#7f1015">A Novel Toolkit for protein-protein interaction (PPI) study</span></span><hr /> | <span><span style="color:#7f1015">A Novel Toolkit for protein-protein interaction (PPI) study</span></span><hr /> | ||
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<span><span style="color:#7f1015">Parts in our Collection</span></span><hr /> | <span><span style="color:#7f1015">Parts in our Collection</span></span><hr /> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span> | ||
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| + | [[File:T--NUDT_CHINA--collectionfig1.jpg|700px|left]] | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;"></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong> PPI Detector</strong></span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong> PPI Detector</strong></span> | ||
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| + | </html> | ||
| + | [[File:T--NUDT_CHINA--collectionfig2.jpg|700px|left]] | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span> | <span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span> | ||
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| + | [[File:T--NUDT_CHINA--collectionfig3.jpg|700px|left]] | ||
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| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Introduction</span> |
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit, | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit, | ||
| − | contained 18 new parts design for protein-protein interaction (PPI) researches. </span> | + | contained 18 new parts design for protein-protein interaction (PPI) researches.</span> |
</p> | </p> | ||
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containing the coding sequence of three split-reporting systems: split-GFP, | containing the coding sequence of three split-reporting systems: split-GFP, | ||
split-Luciferase, and split-HRP. All those basic parts were codon-optimized to | split-Luciferase, and split-HRP. All those basic parts were codon-optimized to | ||
| − | be expressed in | + | be expressed in E.coli, and sequence-modified to meet the demands of RCF10. |
| − | sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining | + | Building on that, amazing parts combining split-reporting system with a novel |
| − | split-reporting system with a novel substitution system were designed, | + | substitution system were designed, characterized and then added into the |
| − | characterized and then added into the collection. Through which two proteins | + | collection. Through which two proteins could be fused with their corresponding |
| − | could be fused with their corresponding split-reporter fragment at the same | + | split-reporter fragment at the same time in one simple Golden-Gate Assembly, |
| − | time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning | + | thus dramatically simplified the cloning process.</span> |
| − | process.</span> | + | |
</p> | </p> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional | <span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional | ||
| − | validated, well characterized and submitted to the registry.</span> | + | validated, well characterized and submitted to the registry.</span> |
| + | </p> | ||
| + | <p> | ||
| + | <br /> | ||
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Revision as of 10:48, 19 October 2016
PAGE STILL UNDER CONSTRUCTION
PPI Toolkit
A Novel Toolkit for protein-protein interaction (PPI) study
Parts in our Collection
N-sGFP/ C-sGFP based fluorescent PPI Detector
| BBa_K1997004 | sGFP-N->Zif268->sGFP-C |
| BBa_K1997015 | P+R->sGFP-N->Zif268->sGFP-C->TER |
| BBa_K1997016 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
| BBa_K1997017 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector
| BBa_K1997014 | GFP10->Zif268->GFP11->RBS->GFP1-9 |
| BBa_K1997018 | P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER |
| BBa_K1997019 | P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER |
| BBa_K1997020 | P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER |
Split-HRP based enzymatic PPI Detector
| BBa_K1997000 | sHRP-N |
| BBa_K1997001 | sHRP-C |
| BBa_K1997005 | sHRP-N->FRB->RBS->FKBP-> sHRP-C |
| BBa_K1997012 | P+R->sHRP-N->Zif268-> sHRP-C->TER |
| BBa_K1997013 | P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER |
Split-Luciferase based enzymatic PPI Detector
| BBa_K1997002 | sLuc-N |
| BBa_K1997003 | sLuc-C |
| BBa_K1997021 | P+R->sLuc-N->Zif268->sLuc-C->TER |
| BBa_K1997022 | P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER |
| BBa_K1997023 | P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER |
Introduction
This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.
In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.
All parts in this kit were functional validated, well characterized and submitted to the registry.
