Difference between revisions of "Team:NUDT CHINA/Part Collection"
Qiuxinyuan12 (Talk | contribs) |
Qiuxinyuan12 (Talk | contribs) |
||
| Line 425: | Line 425: | ||
</html> | </html> | ||
| − | [[File:T--NUDT_CHINA--collectionfig1.jpg| | + | [[File:T--NUDT_CHINA--collectionfig1.jpg|500px|left]] |
<html> | <html> | ||
| Line 448: | Line 448: | ||
</html> | </html> | ||
| − | [[File:T--NUDT_CHINA--collectionfig2.jpg| | + | [[File:T--NUDT_CHINA--collectionfig2.jpg|500px|left]] |
<html> | <html> | ||
| Line 471: | Line 471: | ||
</html> | </html> | ||
| − | [[File:T--NUDT_CHINA--collectionfig3.jpg| | + | [[File:T--NUDT_CHINA--collectionfig3.jpg|500px|left]] |
<html> | <html> | ||
| Line 497: | Line 497: | ||
</html> | </html> | ||
| − | [[File:T--NUDT_CHINA--collectionfig4.jpg| | + | [[File:T--NUDT_CHINA--collectionfig4.jpg|500px|left]] |
<html> | <html> | ||
Revision as of 11:07, 19 October 2016
HomePage • PARTS • Part Collection
Part Collection
Introduction
This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.
In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.
All parts in this kit were functional validated, well characterized and submitted to the registry.
Parts in our Collection
N-sGFP/ C-sGFP based fluorescent PPI Detector
| BBa_K1997004 | sGFP-N->Zif268->sGFP-C |
| BBa_K1997015 | P+R->sGFP-N->Zif268->sGFP-C->TER |
| BBa_K1997016 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
| BBa_K1997017 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector
| BBa_K1997014 | GFP10->Zif268->GFP11->RBS->GFP1-9 |
| BBa_K1997018 | P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER |
| BBa_K1997019 | P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER |
| BBa_K1997020 | P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER |
Split-HRP based enzymatic PPI Detector
| BBa_K1997000 | sHRP-N |
| BBa_K1997001 | sHRP-C |
| BBa_K1997005 | sHRP-N->FRB->RBS->FKBP-> sHRP-C |
| BBa_K1997012 | P+R->sHRP-N->Zif268-> sHRP-C->TER |
| BBa_K1997013 | P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER |
Split-Luciferase based enzymatic PPI Detector
| BBa_K1997002 | sLuc-N |
| BBa_K1997003 | sLuc-C |
| BBa_K1997021 | P+R->sLuc-N->Zif268->sLuc-C->TER |
| BBa_K1997022 | P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER |
| BBa_K1997023 | P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER |
