Difference between revisions of "Team:SVCE CHENNAI/Parts"

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<h1>Parts Collection</h1>
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<h1>OVERVIEW OF PARTS</h1>
 
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Range: BBa_K2113001-BBa_K2113010.
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Range: BBa_K2113001-BBa_K2113010
 
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BASIC PARTS:
 
BASIC PARTS:
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     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113004 and Part BBa_K2113005 are basic parts, coding for the antimicrobial peptides,the former without glycine and the later with glycine. A variant with extra glycine residues is used to increase the half life of the peptide, consistent with the N-end rule. </li>
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113004 and Part BBa_K2113005 are basic parts, coding for the antimicrobial peptides,the former without glycine and the later with glycine. A variant with extra glycine residues is used to increase the half life of the peptide, consistent with the N-end rule. </li>
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<li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; To read more about our basic parts <a href="https://2016.igem.org/Team:SVCE_CHENNAI/Basic_Part">click here</a></li>
 
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COMPOSITE PARTS:
 
COMPOSITE PARTS:
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     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113007 and BBa_K2113008 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a lac promoter, so that the system gets activated in the presence of milk.</li>
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113007 and BBa_K2113008 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a lac promoter, so that the system gets activated in the presence of milk.</li>
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Part BBa_K2113006 contains a strong promoter regulating part BBa_k211301 downstream of which a double terminator is present. BBa_K2113009 and BBa_K2113010 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a RNA thermometer.</li>           
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Part BBa_K2113006 contains a strong promoter regulating part BBa_k211301 downstream of which a double terminator is present. BBa_K2113009 and BBa_K2113010 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a RNA thermometer.</li>           
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<li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;To read more about our composite parts <a href="https://2016.igem.org/Team:SVCE_CHENNAI/Composite_Part">click here</a></li>
 
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<h2>Cost of System for different sizes of Membrane</h2>
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<h2>PARTS COLLECTION:</h2>
 
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Revision as of 00:04, 20 October 2016

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Lactoshield - SVCE_CHENNAI

OVERVIEW OF PARTS


Range: BBa_K2113001-BBa_K2113010

BASIC PARTS:

  •    Parts BBa_K2113004 and Part BBa_K2113005 are basic parts, coding for the antimicrobial peptides,the former without glycine and the later with glycine. A variant with extra glycine residues is used to increase the half life of the peptide, consistent with the N-end rule.
  •    To read more about our basic parts click here
  • COMPOSITE PARTS:

  •    Parts BBa_K2113001 is a composite part coding for a RNA thermometer followed by expression of chromoprotein cj blue,this can be used for the characterization of RNA thermometer under different promoters.
  •    Parts BBa_K2113002 is a composite part that serves as a temperature sensitive system with a lambda pr promoter under cI regulation. BBa_K2113003 similarly is a temperature sensitive system with a RNA thermometer.
  •    Parts BBa_K2113007 and BBa_K2113008 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a lac promoter, so that the system gets activated in the presence of milk.
  •    Part BBa_K2113006 contains a strong promoter regulating part BBa_k211301 downstream of which a double terminator is present. BBa_K2113009 and BBa_K2113010 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a RNA thermometer.
  •   To read more about our composite parts click here

PARTS COLLECTION:


Part Name Part Number
RNA thermometer + cjBlue BBa_K2113001
temperature sensitive system with lambda pr promoter under cI regulation BBa_K2113002
temperature sensitive system with RNA thermometer BBa_K2113003
AMP without glycine BBa_K2113004
AMP with glycine BBa_K2113005
temperature sensitive system with RNA thermometer with cj blue BBa_K2113006
lac inducible AMP without glycine BBa_K2113007
lac inducible AMP with glycine BBa_K2113008
RNA thermometer and AMP without glycine BBa_K2113009
RNA thermometer and AMP with glycine BBa_K2113010