Difference between revisions of "Team:SVCE CHENNAI/Transformation"

(Created page with "{{SVCE_CHENNAI/ramit1}} {{SVCE_CHENNAI/Navbar}} {{SVCE_CHENNAI/homestyle}} {{SVCE_CHENNAI/Foot}} <html> <body> <!-- Section : Workshops --> <section id="workshops"> <div class...")
 
Line 16: Line 16:
 
<div class="row">
 
<div class="row">
 
<div class="col-xs-12 col-sm-12 col-md-12 col-lg-12">
 
<div class="col-xs-12 col-sm-12 col-md-12 col-lg-12">
−
<h2>Requirements for each sample</h2>
+
<h2>Requirements for each sample:</h2>
 
<ul class="points">
 
<ul class="points">
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;1 tube of thawed chemically competent cells (keep covered in ice at all times). </li>
 
     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;1 tube of thawed chemically competent cells (keep covered in ice at all times). </li>
Line 24: Line 24:
 
     </li>
 
     </li>
 
</ul>
 
</ul>
 +
</div>
 +
</div>
 +
<hr class="mainline">
 +
<div class="row">
 +
<div class="col-xs-12 col-sm-12 col-md-12 col-lg-12">
 +
<h2>Procedure:</h2>
 +
<ul class="points">
 +
    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;Add DNA to cells</li>
 +
    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;Incubate on ice for 0-20 minutes</li>
 +
    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;Heat shock at 42 °C for 30 seconds</li>
 +
    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;Incubate on ice for 0-2 minutes
 +
<li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp;Add SOC</li>
 +
    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;Incubate on rotator in 37 °C incubator for 0-60 minutes
 +
    </li>
 +
<li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;Plate 50-250 uL
 +
    </li>
 +
</ul>
 +
</div>
 +
</div>
 +
</section>
 +
</body>
 +
</html>

Revision as of 18:27, 1 December 2016

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Transformation

Requirements for each sample:

  •   1 tube of thawed chemically competent cells (keep covered in ice at all times).
  •   1-10 uL ligation product cloned in plasmid.
  •   250 uL SOC
  •  1 LB plate with appropriate antibiotics

Procedure:

  •   Add DNA to cells
  •   Incubate on ice for 0-20 minutes
  •   Heat shock at 42 °C for 30 seconds
  •  Incubate on ice for 0-2 minutes
  •   Add SOC
  •  Incubate on rotator in 37 °C incubator for 0-60 minutes
  •  Plate 50-250 uL