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Latest revision as of 18:31, 1 December 2016
Transformation
Requirements for each sample:
- 1 tube of thawed chemically competent cells (keep covered in ice at all times).
- 1-10 uL ligation product cloned in plasmid.
- 250 uL SOC
- 1 LB plate with appropriate antibiotics
Procedure:
- Add DNA to cells
- Incubate on ice for 0-20 minutes
- Heat shock at 42 °C for 30 seconds
- Incubate on ice for 0-2 minutes
- Add SOC
- Incubate on rotator in 37 °C incubator for 0-60 minutes
- Plate 50-250 uL
Sponsors
![](https://static.igem.org/mediawiki/2016/9/94/T--SVCE_CHENNAI--s1.png)
![](https://static.igem.org/mediawiki/2016/f/f7/T--SVCE_CHENNAI--s2.png)
![](https://static.igem.org/mediawiki/2016/b/b7/T--SVCE_CHENNAI--s3.png)
![](https://static.igem.org/mediawiki/2016/f/f6/T--SVCE_CHENNAI--s4.png)