Team:NUDT CHINA/Part Collection
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PPI Toolkit
A Novel Toolkit for protein-protein interaction (PPI) study
Parts included in our collection
BBa_K1997000, BBa_K1997001, BBa_K1997002, BBa_K1997003, BBa_K1997004, BBa_K1997005, BBa_K1997012, BBa_K1997013, BBa_K1997014, BBa_K1997015, BBa_K1997016, BBa_K1997017, BBa_K1997018, BBa_K1997019, BBa_K1997020, BBa_K1997021, BBa_K1997022, BBa_K1997023
Introduction
This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.
In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.
All parts in this kit were functional validated, well characterized and submitted to the registry.
