Team:NUDT CHINA/Part Collection
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Part Collection
Introduction
This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.
In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.
All parts in this kit were functional validated, well characterized and submitted to the registry.
Parts in our Collection
N-sGFP/ C-sGFP based fluorescent PPI Detector
| BBa_K1997004 | sGFP-N->Zif268->sGFP-C |
| BBa_K1997015 | P+R->sGFP-N->Zif268->sGFP-C->TER |
| BBa_K1997016 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
| BBa_K1997017 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector
| BBa_K1997014 | GFP10->Zif268->GFP11->RBS->GFP1-9 |
| BBa_K1997018 | P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER |
| BBa_K1997019 | P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER |
| BBa_K1997020 | P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER |
Split-HRP based enzymatic PPI Detector
| BBa_K1997000 | sHRP-N |
| BBa_K1997001 | sHRP-C |
| BBa_K1997005 | sHRP-N->FRB->RBS->FKBP-> sHRP-C |
| BBa_K1997012 | P+R->sHRP-N->Zif268-> sHRP-C->TER |
| BBa_K1997013 | P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER |
Split-Luciferase based chemiluminescence PPI Detector
| BBa_K1997002 | sLuc-N |
| BBa_K1997003 | sLuc-C |
| BBa_K1997021 | P+R->sLuc-N->Zif268->sLuc-C->TER |
| BBa_K1997022 | P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER |
| BBa_K1997023 | P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER |
