Part Collection

Introduction

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This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.

In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.

All parts in this kit were functional validated, well characterized and submitted to the registry.

Parts in our Collection

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N-sGFP/ C-sGFP based fluorescent PPI Detector

GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector

Split-HRP based enzymatic PPI Detector

Split-Luciferase based chemiluminescence PPI Detector