Team:KoreaSonyeodul/Notebook

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  • COLLABORATIONS
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  • BASIC
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  • PUBLIC OUTREACH
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NOTE

Report Journal

Day	Description	Contents	Participants
March 11th	Brainstorming	Contents	All Members
March 18th	Inquiry of potsntial supervisors/campaign report	Contents	All Members
March 23rd	Competition Application	Decide on the subject of the project: Mealworm’s degradation of plastic	All Members
March 25th	Planning	Specifically plan out Experimental Methods and Procedures	All Members
March 29th	Order Mealworms	Order Mealworms	Seunghee Han
March 31st	1st Experiment	4g of Styrofoam, three sub-experiments each have a control and experimental containers with 20 mealworms each: Cool vs. Warm Light vs. No Light With other food vs. Just plastic	All Members
April 7th	2nd Experiment	Check on the progress of the three experiment/document results	Soomin Lee Yemi Shin Jimin Sun Heesun Sim
April 14th	3rd Experiment	Check on the progress of the three experiment/document results	Jungeun Huh Seunghee Han S. Kang
April 21st	4th Experiment	Check on the progress of the three experiment/document results	Soomin Lee Yemi Shin Jimin Sun Heesun Sim
May 15th	Twitter Account/Contact Professor	Created Twitter/Contacted Professor for advice	Yemi Shin
June 1st	Team Meeting	Revise Plans, and further specify plans, but couldn’t work with DNA synthesis because the kit had not arrived. Realizing our significant shortness of genetic knowledge. decided to host a mini-bio forum where we would read the Bio-tech chapter of the Campbell textbook and present	All Members
June 13th	Mini-Bio Forum	Each one of us presented different sections of the Bio-Tech chapter.	All Members
July 13th	Arrival of the DNA kit	DNA kit finally, finally arrived	Soomin Lee
July 8th	1st Visit to Professor	Got advice for our project in the future, talked about ways in which the lab could support our project	All Members
July 8th	Collaboration with Korea University Team	Presentation of both team’s subjects and time for giving advice	All Members
July 11th	Meeting with Professor Cho	Before this official meeting, we only had communications throughout e-mails with our instructor, who is a synthetic biology professor in Korea University. In this meeting in the Korea University, we specified the experiments that we will conduct. Then, we planned the specific dates of experiment	All Members
August 12th	Planning the experiment	Seunghee Han and Yemi Shin visited the Korea University to discuss about how we are going to further the experiment. At first our team put the main focus on enhancing the speed of degradation. However, after talking with professor Choi, we’ve decided to recombine the mealworm’s gut dna, DH5+alpha and PUC 19. We also scheduled our experiments	Seunghee Han Yemi Shin
August 16th	Chemical Transformation	We transfomed E-coli, DH5-alpha using PUC19 in Korea University	Seunghee Han Soomin Lee
August 26th	Mealworm Related Measurements	After feeding 40 mealworms with only plastic for 5 weeks, we measured the length and the weight of the 20 samples. The average weight is 0.177g per worm and the original length is about 3+- 0.4cm. Compared to the original length 2+-0.2cm, it is noticeable that mealworms are possible to continue their lives with plastics as their only source of nutrient	S. Kang Jungeun Huh Jimin Sun
September 9th		Visited the Korea University to practice E-coli cell streaking. Did the three sector T streak. Organisms are streaked through the first sector using either a disposable loop or needle, or a reusable loop, usually with nichrome or platinum wire, and then passed through a Bunsen burner for sterilization		Seunghee Han Yemi Shin
September 22nd	Chemical Transformation of E.coli	Visited Korea University and experimented the chemical transformation of the e.coli strain DH5 alpha and pBBRMCS2-Pcon-yfaL-PETase	Seunghee han Jeongeun huh
October 7th	Checking and Marking off the Judging form	Together with our TA, we completed our Judging Forms and organized all the final materials needed for the Giant Jamboree	Soomin Lee Seohyun Kang
October 10th	Semi-final check	Again with the TA we submitted all the parts to the registry (PETase) and double checked the wiki	Yemi Shin JImin Sun
October 16th	Final check	Final check of the wiki, made revisions	Yemi Shin Heesun Sim