Part name
BBa_K1999000 (PETase)
Part type
Protein coding sequence
Creator
Seung-Woo Baek
Sequence
ATGAACTTTCCTCGCGCCTCCCGCTTGATGCAAGCAGCAGTCCTGGGAGGTTTAATGGCGGTCAGCGCTGCGGCGACGGCACAGACGAATCCTTACGCTCGTGGTCCAAACCCGACGGCAGCCAGTTTGGAAGCATCGGCTGGGCCTTTCACTGTCCGCAGCTTTACTGTTTCCCGCCCCTCTGGATATGGCGCAGGGACTGTCTATTACCCGACCAACGCGGGGGGCACGGTAGGAGCTATTGCAATCGTCCCCGGATACACTGCACGTCAGTCATCCATCAAGTGGTGGGGACCTCGTTTGGCCAGTCACGGTTTTGTCGTGATTACCATTGACACCAACTCAACCTTGGATCAGCCGAGCTCCCGTTCAAGTCAACAGATGGCCGCGTTGCGTCAGGTAGCCAGCCTGAACGGCACGAGCAGTTCACCTATCTATGGCAAAGTGGACACCGCGCGCATGGGAGTCATGGGCTGGTCTATGGGCGGGGGTGGAAGTCTGATTAGCGCGGCGAATAACCCCAGCTTAAAGGCAGCTGCGCCTCAAGCCCCTTGGGACTCGTCCACGAATTTCTCTTCTGTCACCGTACCCACCCTTATTTTTGCTTGCGAAAATGATTCCATCGCCCCGGTAAATTCATCGGCGTTACCAATTTATGACTCGATGAGCCGCAACGCCAAGCAATTCCTGGAGATTAATGGGGGGAGTCATTCGTGTGCAAATAGTGGAAACTCCAACCAGGCATTAATCGGCAAAAAGGGGGTAGCTTGGATGAAGCGTTTCATGGACAACGACACGCGTTACTCAACTTTCGCCTGTGAAAACCCAAATTCCACGCGCGTAAGCGATTTCCGTACCGCAAATTGCTCCTAA
Short description
Forms PETase which degrades PET.
Long description
Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Is also able to hydrolyze bis(hydroxyethyl) terephthalate (BHET) to yield MHET with no further decomposition. Shows low activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) in vitro
