Q1. Please describe the chassis organism (s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
|Species||Strain no/name||TRisk Group||Risk group source||Disease risk to humans?If so, which disease?|
|E. col||BL21||1||Laboratory Biosafety Mannual Third Edition by World Health Organization 2004||Yes.May cause irritation to skin.|
|E. coli||DH5-alpha||1||Laboratory Biosafety Mannual Third Edition by World Health Organization 2004||No.|
|S.cerevisiae||BY4741||1||Laboratory Biosafety Mannual Third Edition by World Health Organization 2004||No.|
Q2. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2015 iGEM Distribution without modifying them, you do not need to list those parts.)
|Part number||Name||Where did you get the physical DNA for this part (which lab, synthesis company, etc)||What species does this part originally come from?||What is the Risk Group of the species?||What is the function of this part, in its parent species?|
|BBa_K1982012||vp64||Synthesized, GENEWIZ||N/A||N/A||Tetrameric VP16 transcription activator domain|
|BBa_K1982001||tCas9||Purchased from GENESHARE||Streptococcus Pyogenes||2||Binding protein|
|BBa_K1982002||CRY2||Made by our own lab (Reverse transcription)||Arabidopsis thaliana||N/A||Blue light stimulated photoreceptor|
|BBa_K1982003||CIB1||Made by our own lab (Reverse transcription)||Arabidopsis thaliana||N/A||Transcription factor|
Q3. What is the Safety Level of your lab?
Level 1 (low risk)
Q4. Which work areas do you use to handle biological materials?
Open bench, biosafety cabinet / laminar flow hood and chemical fume hood.
Q5. a) Have your team members received any safety training yet?
Yes, we have already received safety training.
Q5. b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.
We learned about the regulations of laboratory safety of our institution, standard experimental protocols, how to use the equipments correctly, identification of a variety of chemical reagents, how to deal with microorganism correctly, etc.
Q5. c) Who provided your safety training? Did you get training from multiple people? (For example: university biosafety office, your team PIs/instructors, etc.)
Our team advisors and instructors. Because we are performing experiments in professor Ding's lab, students who work there can guide us if we meet acoross some troubles. We also have had some certain courses teached by different teachers about biosafety ever before since we all major in bioengineering.
Q6. Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
Zhanyou Wang who is the vice president of our college (College of Life Science and Health, Northeastern Universtiy). Here are some guidelines of laboratory safety regulations:
- Every students should consciously abide by the laboratory disciplines and safeguard the order of the laboratory.
- Must carefully prepare before experiments, familiar with the purpose of this experiment, principle, operation steps, Understand the significance of each operation steps and understand the use of instruments, otherwise you can't start the experiment.
- Experim ental mesa should be clean and tidy, and instruments, medicines should be placed neatly. Common reagents should be covered tightly in place immediately after used up. Don't make the reagents, and drug sprinkled on experimental mesa. You are not be allowed to leave the laboratory untill you clean the mesa and ensure all the instruments are turned off.
- Waste liquid will be poured into the water tank with water at the same time. Must use strong acid, strong alkali solution diluted by water. Waste paper and other solid waste can be throwed into a cylinder which can be dealt after experiment. Don't throw Into the sink or throw everywhere.
- To achieve sterilization, open uv lamp for 30 to 60 minutes in sterile room space for 30 to 60 minutes.
- Check the equipment, must be separately sterilized before moving in sterile room.
- Dress white coats, sterile working clothes, gloves.
- You have to sterilize your hands before the operation in aseptic environment .
- Movements should be light and not too fast to avoid pollution. Move glasses softly in order to avoid damage.
- Must operate in accordance with the relevant provisions of sterile operation.
- All discharges of discarded reagents and microbial must be treated as harmless.
- If you are uncomfortable in the process of experiment, immediately stop experiment.
- Pay attention during animal feeding, wear work clothes and gloves, if you are bitten, go to hospital immediately.
- Laboratory medicine can not be medical use and shall not be eaten.
Q7. In your country / region, what are the laws and regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
Here are some links to regulations concerning biosafety in research laboratories in China:
This is our national laws about biosafety.
This is a book about the standard requirments of laboratory safety and it refers to lots of international biosafety regulations such as :
- The Minister ofHealth, Canada. The Laboratory Biosafety Guidelines [M]. 3rd ed. Ottawa: HealthCanada, 2004.U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institutes of Health.
- Biosafety in Microbiological and Biomedical Laboratories[M].5th ed. Washington: U. S.Government Printing Office, 2007
- Microbiological aspects and containment facilities[S]. New Zealand: StandardsAustralia, 2002 European Committee for Standardization.
- Determining leakage of filter element (Scan method) [S].Brussels: European Committee for Standardization, 2000.
- Laboratory biorisk management standard[S]. Brussels: European Committee for Standardization, 2008.
- Medical laboratories-Particular requirements for quality and competence [S]. Geneva: International Organization for Standards, 2007.
- Containment enclosures: Part 2: Classification according to leak tightness and associated checking methods [S].Geneva:International Organization for Standards, 1994.
- Laboratory biosafety manual [M]. 3rd ed.Geneva:World Health Organization, 2004.
This is a book about General biosafety standard for microbiological and biomedical laboratories