Difference between revisions of "Team:Pittsburgh/Project Overview"
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.DNAzyme { | .DNAzyme { | ||
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background: url("https://static.igem.org/mediawiki/2016/0/0f/T--Pittsburgh--Overview_DNAzyme_static.jpg"); | background: url("https://static.igem.org/mediawiki/2016/0/0f/T--Pittsburgh--Overview_DNAzyme_static.jpg"); | ||
/*margin:auto; /*position the [div] on the center*/*/ | /*margin:auto; /*position the [div] on the center*/*/ | ||
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.imgWrap { | .imgWrap { | ||
position:relative; | position:relative; | ||
| − | height: | + | height:200px; |
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float:left; | float:left; | ||
display: inline-block; | display: inline-block; | ||
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<div class="imgWrap"> | <div class="imgWrap"> | ||
| − | <a href="#DNAzyme" class="table"><img src="" alt="DNAzyme" style="width: | + | <a href="#DNAzyme" class="table"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--Pittsburgh--Overview_DNAzyme_static.jpg" alt="DNAzyme" style="width:300px;padding:5px;"></a><span class="imgDescription">DNAzyme</span></div> |
| − | + | <div class="imgWrap"> | |
| − | <a href="#switch" class="table"><img src="" alt="Switch" style="width: | + | <a href="#switch" class="table"><img src="https://static.igem.org/mediawiki/2016/2/22/T--Pittsburgh--Overview_Switch_static.jpg" alt="Switch" style="width:300px;padding:5px;"></a><span class="imgDescription">Toehold Switch</span></div> |
<div class="imgWrap"> | <div class="imgWrap"> | ||
| − | <a href="#reporter" class="table"><img src="" alt="Reporter" style="width: | + | <a href="#reporter" class="table"><img src="https://static.igem.org/mediawiki/2016/8/82/T--Pittsburgh--Overview_Reporter_static.jpg" alt="Reporter" style="width:300px;padding:5px;"></a><span class="imgDescription">Reporter</span></div> |
<div class="imgWrap"> | <div class="imgWrap"> | ||
| − | <a href="#amplifier" class="table"><img src="" alt="Amplifier" style="width: | + | <a href="#amplifier" class="table"><img src="https://static.igem.org/mediawiki/2016/4/41/T--Pittsburgh--Overview_Amplifier_static.jpg" alt="Amplifier" style="width:300px;padding:5px;"></a><span class="imgDescription">Amplifier</span></div> |
</ul> | </ul> | ||
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<span class="anchor" id="intro"></span> | <span class="anchor" id="intro"></span> | ||
<h1>Hot Metal Switch</h1> | <h1>Hot Metal Switch</h1> | ||
| − | <p>Hot Metal Switch is a cell-free sensor that conditionally expresses <i>lacZ</i> only in the presence of the target metal, either lead or thallium. The metal ion detection is based on specific DNAzymes that are cleaved in the presence of their target metal. The released DNA strand activates a toehold switch regulated by the T7 promoter system, which will promote transcription of T3 RNA polymerase. The T3 RNA polymerase will activate the expression of <i>lacZ</i>, which will produce a color change visible to the naked eye. The T3 RNA polymerase will also activate additional expression of itself to amplify the original signal from the DNAzyme cleavage.</p> | + | <p>Hot Metal Switch is a cell-free sensor that conditionally expresses <i>lacZ</i> only in the presence of the target metal, either lead or thallium. The metal ion detection is based on specific DNAzymes that are cleaved in the presence of their target metal. The released DNA strand activates a toehold switch regulated by the T7 promoter system, which will promote transcription of T3 RNA polymerase. The T3 RNA polymerase will activate the expression of <i>lacZ</i>, which will produce a color change visible to the naked eye. The T3 RNA polymerase will also activate additional expression of itself to amplify the original signal from the DNAzyme cleavage.</p><br> |
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="in_vitro"></span> | <span class="anchor" id="in_vitro"></span> | ||
<h1><i>In Vitro</i> Protein Synthesis</h1> | <h1><i>In Vitro</i> Protein Synthesis</h1> | ||
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="circuit"></span> | <span class="anchor" id="circuit"></span> | ||
<h1>Genetic Circuit</h1> | <h1>Genetic Circuit</h1> | ||
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="DNAzyme"></span> | <span class="anchor" id="DNAzyme"></span> | ||
<h2>DNAzyme</h2> | <h2>DNAzyme</h2> | ||
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="switch"></span> | <span class="anchor" id="switch"></span> | ||
<h2>Toehold Switch</h2> | <h2>Toehold Switch</h2> | ||
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="reporter"></span> | <span class="anchor" id="reporter"></span> | ||
<h2>Reporter</h2> | <h2>Reporter</h2> | ||
| + | <a href="#Top">Back to Top</a> | ||
<span class="anchor" id="amplifier"></span> | <span class="anchor" id="amplifier"></span> | ||
<h2>Amplifier</h2> | <h2>Amplifier</h2> | ||
| + | <a href="#Top">Back to Top</a> | ||
Revision as of 02:55, 4 September 2016
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Contents
- Hot Metal Switch
- In Vitro Protein Synthesis
- Genetic Circuit
DNAzyme
Toehold Switch
Reporter
AmplifierHot Metal Switch
Hot Metal Switch is a cell-free sensor that conditionally expresses lacZ only in the presence of the target metal, either lead or thallium. The metal ion detection is based on specific DNAzymes that are cleaved in the presence of their target metal. The released DNA strand activates a toehold switch regulated by the T7 promoter system, which will promote transcription of T3 RNA polymerase. The T3 RNA polymerase will activate the expression of lacZ, which will produce a color change visible to the naked eye. The T3 RNA polymerase will also activate additional expression of itself to amplify the original signal from the DNAzyme cleavage.
Back to Top
In Vitro Protein Synthesis
Back to TopGenetic Circuit
Back to TopDNAzyme
Back to TopToehold Switch
Back to TopReporter
Back to TopAmplifier
Back to Top