Difference between revisions of "Team:NAU-CHINA/Description"

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<p>The main metabolite in the degradation of insecticide pyrethroids in mammals, insects, fungi, and bacteria, is <strong>3-phenoxybenzoate (3-PBA)</strong>, a diaryl ether compound.For the wide use of pyrethroids and the stability of the diaryl ether compound itself, 3-PBA is typically detected as an important environmental contaminant. </p>
  
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<p><b><i>Sphingobium wenxiniae</i> JZ-1 ͭ  </b>can utilize 3-PBA as the sole carbon and energy sources for growth,with the 3-phenoxybenzoate (3-PBA) 1,2-dioxygenase gene cluster (pbaA1A2B cluster) and the reductase component gene pbaC, which are responsible for catalyzing 3-phenoxybenzoate(3-HBA)to 3-hydroxybenzoate and catechol. Also genes(mhbDHIM), from Klebsiella pneumoniae M5a1 which is capable of utilizing 3-hydroxybenzoate, encode enzymes converting 3-hydroxybenzoate (3-HBA) to pyruvate and fumarate via gentisate.</p>
<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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<p>Therefore, our project is to design a device with engineered bacterias which are capable of degrading 3-PBA more efficiently.</p>
  
  
 
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Revision as of 14:23, 13 June 2016

The main metabolite in the degradation of insecticide pyrethroids in mammals, insects, fungi, and bacteria, is 3-phenoxybenzoate (3-PBA), a diaryl ether compound.For the wide use of pyrethroids and the stability of the diaryl ether compound itself, 3-PBA is typically detected as an important environmental contaminant.

Sphingobium wenxiniae JZ-1 ͭ can utilize 3-PBA as the sole carbon and energy sources for growth,with the 3-phenoxybenzoate (3-PBA) 1,2-dioxygenase gene cluster (pbaA1A2B cluster) and the reductase component gene pbaC, which are responsible for catalyzing 3-phenoxybenzoate(3-HBA)to 3-hydroxybenzoate and catechol. Also genes(mhbDHIM), from Klebsiella pneumoniae M5a1 which is capable of utilizing 3-hydroxybenzoate, encode enzymes converting 3-hydroxybenzoate (3-HBA) to pyruvate and fumarate via gentisate.

Therefore, our project is to design a device with engineered bacterias which are capable of degrading 3-PBA more efficiently.