Team:NAU-CHINA/Part Collection
Best Composite Part
This collection is used to degrade 3-PBA thoroughly.
Figure 1.The sketch map of 3-PBA biodegradation process.
SectionⅠ pba gene cluster
| pba | pbaA1A2 | 1.pbaA1A2(BBa_K2088003); 2.nativeRBS-pbaA1A2B+dT(BBa_K2088016) |
| pbaB | 3.pbaB(BBa_K2088007) | |
| pbaC | 4.pbaC+dT(BBa_K2088005); 5.B0030+UTR-pbaC (BBa_K2088668); 6.B0032+UTR-pbaC(BBa_K2088669); 7.J23100+B0034+ pbaC+dT(BBa_K2088017); 8.J23100+B0030+ pbaC+dT(BBa_K2088018) |
SectionⅡ mhb gene cluster
| mhb | 9.mhbT(BBa_K2088666) |
| 10.copA+riboJ+mhbDHIM+dT(BBa_K2088672) |
Section Ⅲ C230
| C23O | 11.C23O(BBa_K2088000) |
| 12.nativeRBS-C23O(BBa_K2088008) 13.nativeRBS-C23O+dT(BBa_K2088009) |
Section Ⅳ Regulatory factors
| mhb operon | mhbR | 14.mhbR(BBa_K2088011); 15.optimized mhbR(BBa_K2088670) |
| mhbPt | 16.mhbPt(BBa_K2088013); 17.mhbPt+GFP(BBa_K2088004); 18.mhbPt(left)+dT(BBa_K2088612); 19.mhbPt(left)+GFP(BBa_K2088689) |
|
| mhbR+mhbPt |
20.mhbR+mhbPt(BBa_K2088012); 21.dT+mhbR+mhbPt+GFP(BBa_K2088002); 22.J23100+B0034+mhbT+dT(BBa_K2088671)+dT+mhbR+mhbPt+GFP(BBa_K2088616) |
|
| copA | 23.copA(BBa_K2088001) | |
| J23101+riboJ+M6+GFP+dT | 24.J23101+riboJ+M6+GFP+dT(BBa_K2088014) |
