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Revision as of 14:42, 18 June 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

Our weekly progress

Contents

Week 1: May 23 - May 27

Wet Lab

  • Training begins
  • Grow Top 10 competent cells.

Dry Lab

  • Brainstorm genetic circuits for a thallium sensor
  • Lab safety training
Week 1 Notebook Back to Top

Week 2: May 31 - June 3

Wet Lab

  • Test efficiency of competent cells
  • Test cell free extract reaction with T7-GFP plasmid

Dry Lab

  • Contact museums and summer programs for outreach opportunities
  • Lab safety training
Week 2 Notebook Back to Top

Week 3: June 6 - June 12

Wet Lab

  • Transform T7 promoter, amilCP, and terminator
  • Begin assembly by ligating linearized T7 promoter and amilCP

Dry Lab

  • Contact museums and summer programs for outreach opportunities
Week 3 Notebook Back to Top

Week 4: June 13 - June 17

Wet Lab

  • Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
  • Send promising T7 promoter -- amilCP ligations to be sequenced
  • Perform double digest of T7 promoter and terminator from last week
  • Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)

Dry Lab

  • TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
Week 4 Notebook Back to Top