Difference between revisions of "Team:NCKU Tainan/Safety"
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| − | <div class="head">JUDGING / Safety | + | <div class="head">JUDGING / Safety</div> |
<div class="content row"> | <div class="content row"> | ||
<div class="col-md-9"> | <div class="col-md-9"> | ||
| + | <div class="head2">Safety</div> | ||
| + | <div class="title-line" id="method">Safety Issues</div> | ||
| + | <h5>How did we address the safety issues in our project?</h5> | ||
| + | <ul><li>A <b>non-pathogenic chassis</b>, i.e. <i>E.coli DH5α</i>, <i>E.coli BL21</i> (DE3), is chosen.</li> | ||
| + | <li>The chassis is in small <b>enclosed space</b> as a barrier to prevent contamination.</li> | ||
| + | <li><b>"Suicide-gene inducers"</b> in the enclosed space are designed to kill <i>E.coli</i> in the bio-sensing strip.</li> | ||
| + | </ul> | ||
| + | <div class="title-line" id="plan">Safety Plan</div> | ||
| + | <p>Our <i>E.coli</i> can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the pBAD promoter (<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a>) and then activate the transcription of Lysis protein (<a href="http://parts.igem.org/Part:BBa_K117000">BBa_K117000</a>).</p> | ||
| + | <img src="/wiki/images/4/4b/T--NCKU_Tainan--safety0.jpg" style="margin-bottom:0"> | ||
| + | <div class="img" style="width:24%;display:inline-block;font-weight:bold"> | ||
| + | <img src="/wiki/images/2/2e/T--NCKU_Tainan--safety1.jpg"> | ||
| + | <div style="color:#0d0d0d">RO water</div> | ||
| + | </div> | ||
| + | <div class="img" style="width:24%;display:inline-block;font-weight:bold"> | ||
| + | <img src="/wiki/images/0/00/T--NCKU_Tainan--safety2.jpg"> | ||
| + | <div style="color:#f5474a">Glu 40mM</div> | ||
| + | </div> | ||
| + | <div class="img" style="width:24%;display:inline-block;font-weight:bold"> | ||
| + | <img src="/wiki/images/6/68/T--NCKU_Tainan--safety3.jpg"> | ||
| + | <div style="color:#28c22c">Ara 40mM</div> | ||
| + | </div> | ||
| + | <div class="img" style="width:24%;display:inline-block;font-weight:bold"> | ||
| + | <img src="/wiki/images/4/43/T--NCKU_Tainan--safety4.jpg"> | ||
| + | <div style="color:#1e9db3">Ara 40mM+ Glu 40mM</div> | ||
| + | </div> | ||
| + | <div class="title-line" id="lab">Safe Lab</div> | ||
| + | <h5>Build a safe lab</h5> | ||
| + | <p>We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes. </p> | ||
| + | <div class="title-line" id="law">Laws and Regulations</div> | ||
| + | <h5>Obeying the laws and regulations of biosafety in our Country/ University</h5> | ||
| + | <p>The Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at Nation Cheng-Kung University (<a href="http://epsh.ncku.edu.tw/bin/downloadfile.php?file=WVhSMFlXTm9Memt6TDNCMFlWODBPVEU0TTE4NE9EZzBOVGN5WHpJNE9USTNMbkJrWmc9PQ==&fname=NTVTZjU0bXA1YTZKNVlXbzU2eXM1TGlBNTYySjU3U2E2SWV6NTZ5czVMaUo1NjJKNTdTYTVhK202YW1YNWE2azVhNko1WVdvNkthUDU2K0VMVEl3TVRNd016QTFMbkJrWmc9PQ==">Guidelines for Laboratory Biosafety</a>). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (<a href="http://epsh.ncku.edu.tw/bin/downloadfile.php?file=WVhSMFlXTm9MekU1TDNCMFlWODFNVE0xT1Y4M09UazFOamRmTkRJM01qTXVjR1Jt&fname=NWErbTZhbVg1YTZrNTVTZjU0bXA1YTZKNVlXbzU2Nmg1NUNHNXJPVjZLYVA1WStLNktHTTVwUy81b3lINWJDTzViMlo1N2VvTXVlSmlDamxyb3pucUwvbW9LSGxzSTBwNTVhKzU2Nmg1NzJ5TWpBeE5TNHhNUzR4TVM1d1pHWT0=">Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance</a>).</p> | ||
| + | <div class="title-line" id="shipment">Safe Shipment</div> | ||
| + | TODO: 中文字要改掉 | ||
| + | <p>Did you face any safety problems in sending your DNA parts to the Registry? | ||
| + | How did you solve those problems? | ||
| + | 寄送貨運公司? 注意事項? 是否需要準備申請文件之類的前置作業? </p> | ||
| + | |||
| + | </div> | ||
| + | <div class="col-md-3"> | ||
| + | <ul id="sidemenu"> | ||
| + | <li><a href="#" onclick="return toEvent('method');">Safety Issues</a></li> | ||
| + | <li><a href="#" onclick="return toEvent('plan');">Safety Plan</a></li> | ||
| + | <li><a href="#" onclick="return toEvent('lab');">Safety Lab</a></li> | ||
| + | <li><a href="#" onclick="return toEvent('law');">Laws, Regulations</a></li> | ||
| + | <li><a href="#" onclick="return toEvent('shipment');">Safety Shipment</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
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Revision as of 19:47, 9 October 2016
JUDGING / Safety
Safety
Safety Issues
How did we address the safety issues in our project?
- A non-pathogenic chassis, i.e. E.coli DH5α, E.coli BL21 (DE3), is chosen.
- The chassis is in small enclosed space as a barrier to prevent contamination.
- "Suicide-gene inducers" in the enclosed space are designed to kill E.coli in the bio-sensing strip.
Safety Plan
Our E.coli can sense urea glucose. When the glucose level is high, the bacteria can express red fluorescent protein (RFP) as a reporter to give a signal which can be detected using excitation. After the detection, the “suicide-gene inducers”, arabinose, induce the pBAD promoter (BBa_K206000) and then activate the transcription of Lysis protein (BBa_K117000).
RO water
Glu 40mM
Ara 40mM
Ara 40mM+ Glu 40mM
Safe Lab
Build a safe lab
We operated the project in the Biosafety Level 1 (BSL-1) Lab. The space is suitable for experiments in all of our chassis which are Risk Group 1 organisms. Additionally, we always handled biological materials in laminar flow hood. All of biowastes were autoclaved at the typical 121 °C/15 psi for 15 minutes.
Laws and Regulations
Obeying the laws and regulations of biosafety in our Country/ University
The Environmental Protection, Safety and Health Center is responsible for the safety of biology labs at Nation Cheng-Kung University (Guidelines for Laboratory Biosafety). And Centers for Disease Control (CDC) is the department to govern biosafety for research laboratories in our country (Laboratory Biosafety Management: A Compilation of Regulations and Administrative Guidance).
Safe Shipment
TODO: 中文字要改掉
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems? 寄送貨運公司? 注意事項? 是否需要準備申請文件之類的前置作業?
