Difference between revisions of "Team:Pittsburgh/Notebook"
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<li>Test efficiency of competent cells</li> | <li>Test efficiency of competent cells</li> | ||
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| + | <h3>Cell Free Extract</h3> | ||
| + | <ul><li>Test cell free extract reaction with T7-GFP plasmid</li></ul> | ||
<h2>Dry Lab</h2> | <h2>Dry Lab</h2> | ||
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<h1 class="nav"><a name="Week3" class="nav">Week 3: June 6 - June 12</a></h1> | <h1 class="nav"><a name="Week3" class="nav">Week 3: June 6 - June 12</a></h1> | ||
<h2>Wet Lab</h2> | <h2>Wet Lab</h2> | ||
| + | <h3>Reporter</h3> | ||
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<li>Transform T7 promoter, amilCP, and terminator</li> | <li>Transform T7 promoter, amilCP, and terminator</li> | ||
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<h1 class="nav"><a name="Week4" class="nav">Week 4: June 13 - June 17</a></h1> | <h1 class="nav"><a name="Week4" class="nav">Week 4: June 13 - June 17</a></h1> | ||
<h2>Wet Lab</h2> | <h2>Wet Lab</h2> | ||
| + | <h3>Reporter</h3> | ||
<ul> | <ul> | ||
<li>Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP</li> | <li>Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP</li> | ||
Revision as of 18:58, 26 June 2016
Contact Us
Thank you to our sponsors!
Our weekly progress
Week 1: May 23 - May 27
Wet Lab
- Training begins
- Grow Top 10 competent cells.
Dry Lab
- Brainstorm genetic circuits for a thallium sensor
- Lab safety training
Week 2: May 31 - June 3
Wet Lab
- Test efficiency of competent cells
Cell Free Extract
- Test cell free extract reaction with T7-GFP plasmid
Dry Lab
- Contact museums and summer programs for outreach opportunities
- Lab safety training
Week 3: June 6 - June 12
Wet Lab
Reporter
- Transform T7 promoter, amilCP, and terminator
- Begin assembly by ligating linearized T7 promoter and amilCP
Dry Lab
- Contact museums and summer programs for outreach opportunities
Week 4: June 13 - June 17
Wet Lab
Reporter
- Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
- Send promising T7 promoter -- amilCP ligations to be sequenced
- Perform double digest of T7 promoter and terminator from last week
- Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)
Dry Lab
- TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
