Difference between revisions of "Team:Pittsburgh/Notebook"
| Line 16: | Line 16: | ||
<li><a href="#Week3" class="table">Week 3: June 6 - June 12</a></li> | <li><a href="#Week3" class="table">Week 3: June 6 - June 12</a></li> | ||
<li><a href="#Week4" class="table">Week 4: June 13 - June 17</a></li> | <li><a href="#Week4" class="table">Week 4: June 13 - June 17</a></li> | ||
| + | <li><a href="#Week5" class="table">Week 5: June 20 - June 26</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 31: | Line 32: | ||
<li>Lab safety training</li> | <li>Lab safety training</li> | ||
</ul> | </ul> | ||
| − | <a href=https://static.igem.org/mediawiki/2016/b/bc/TeamPittsburghNotebookWeek1.pdf target="_blank">Week 1 Notebook</a> | + | <a href=https://static.igem.org/mediawiki/2016/b/bc/TeamPittsburghNotebookWeek1.pdf target="_blank">Week 1 Notebook</a><br> |
<a href="#Top">Back to Top</a> | <a href="#Top">Back to Top</a> | ||
| Line 46: | Line 47: | ||
<li>Lab safety training</li> | <li>Lab safety training</li> | ||
</ul> | </ul> | ||
| − | <a href=https://static.igem.org/mediawiki/2016/a/a8/TeamPittsburghNotebookWeek2.pdf target="_blank">Week 2 Notebook</a> | + | <a href=https://static.igem.org/mediawiki/2016/a/a8/TeamPittsburghNotebookWeek2.pdf target="_blank">Week 2 Notebook</a><br> |
<a href="#Top">Back to Top</a> | <a href="#Top">Back to Top</a> | ||
| Line 60: | Line 61: | ||
<li>Contact museums and summer programs for outreach opportunities</li> | <li>Contact museums and summer programs for outreach opportunities</li> | ||
</ul> | </ul> | ||
| − | <a href=https://static.igem.org/mediawiki/2016/b/b3/TeamPittsburghNotebookWeek3.pdf target="_blank">Week 3 Notebook</a> | + | <a href=https://static.igem.org/mediawiki/2016/b/b3/TeamPittsburghNotebookWeek3.pdf target="_blank">Week 3 Notebook</a><br> |
<a href="#Top">Back to Top</a> | <a href="#Top">Back to Top</a> | ||
| Line 76: | Line 77: | ||
<li>TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set</li> | <li>TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set</li> | ||
</ul> | </ul> | ||
| − | <a href=https://static.igem.org/mediawiki/2016/7/75/TeamPittsburghNotebookWeek4.pdf target="_blank">Week 4 Notebook</a> | + | <a href=https://static.igem.org/mediawiki/2016/7/75/TeamPittsburghNotebookWeek4.pdf target="_blank">Week 4 Notebook</a><br> |
| + | <a href="#Top">Back to Top</a> | ||
| + | |||
| + | <h1 class="nav"><a name="Week5" class="nav">Week 5: June 20 - June 26</a></h1> | ||
| + | <h2>Wet Lab</h2> | ||
| + | <h3>Reporter</h3> | ||
| + | <ul> | ||
| + | <li>Ligate T7 promoter -- amilCP construct to terminator</li> | ||
| + | <li>Extract successful ligations of T7 promoter to eGFP</li> | ||
| + | <li>Ligate T7 promoter -- eGFP construct to terminator</li> | ||
| + | </ul> | ||
| + | <h3>Cell Free Extract</h3> | ||
| + | <ul> | ||
| + | <li>Reaction volume can be reduced to 5 μL with 5 ng/μL of DNA</li> | ||
| + | </ul> | ||
| + | <h3>Toehold Switch</h3> | ||
| + | <ul> | ||
| + | <li>Collins triggers activate the switches (both in plasmid form) to express LacZ</li> | ||
| + | </ul> | ||
| + | <h2>Dry Lab</h2> | ||
| + | <ul> | ||
| + | <li>Reach out to teams to collaborate based on last year's projects</li> | ||
| + | </ul> | ||
| + | <a href= target="_blank">Week 5 Notebook</a><br> | ||
<a href="#Top">Back to Top</a> | <a href="#Top">Back to Top</a> | ||
Revision as of 19:14, 26 June 2016
Contact Us
Thank you to our sponsors!
Our weekly progress
Week 1: May 23 - May 27
Wet Lab
- Training begins
- Grow Top 10 competent cells.
Dry Lab
- Brainstorm genetic circuits for a thallium sensor
- Lab safety training
Back to Top
Week 2: May 31 - June 3
Wet Lab
- Test efficiency of competent cells
Cell Free Extract
- Test cell free extract reaction with T7-GFP plasmid
Dry Lab
- Contact museums and summer programs for outreach opportunities
- Lab safety training
Back to Top
Week 3: June 6 - June 12
Wet Lab
Reporter
- Transform T7 promoter, amilCP, and terminator
- Begin assembly by ligating linearized T7 promoter and amilCP
Dry Lab
- Contact museums and summer programs for outreach opportunities
Back to Top
Week 4: June 13 - June 17
Wet Lab
Reporter
- Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
- Send promising T7 promoter -- amilCP ligations to be sequenced
- Perform double digest of T7 promoter and terminator from last week
- Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)
Dry Lab
- TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
Back to Top
Week 5: June 20 - June 26
Wet Lab
Reporter
- Ligate T7 promoter -- amilCP construct to terminator
- Extract successful ligations of T7 promoter to eGFP
- Ligate T7 promoter -- eGFP construct to terminator
Cell Free Extract
- Reaction volume can be reduced to 5 μL with 5 ng/μL of DNA
Toehold Switch
- Collins triggers activate the switches (both in plasmid form) to express LacZ
Dry Lab
- Reach out to teams to collaborate based on last year's projects
Back to Top
