Difference between revisions of "Team:NAU-CHINA/Safety"

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{{NAU-CHINA}}
 
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
  
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<li><a href="https://2016.igem.org/Team:NAU-CHINA/Safety"> SAFETY</a></li>
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<a href="https://2016.igem.org/Team:NAU-CHINA/Human_Practices"> HP</a>
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<li><a href="https://2016.igem.org/Team:NAU-CHINA/Safety">SAFETY</a></li>
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<div id="img01"><img src="https://static.igem.org/mediawiki/2016/c/c9/NAU_CHINA_SAFETY_PIC1.jpeg"><b><br>Pic.1 General Safety Matters</b></div>
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<div id="zhuyaoneirong">
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<h1>Details</h1>
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<h2>1.Lab safety</h2>
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<h3>1-1.Laboratory daily etiquette</h3>
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<p class="second">No matter where we have to show repect to each other. Like removing the glove from your hand when you open the door avoiding staining it. Many details have to be amphasised but hard to list.</p>
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<h4>1-1-1. Safety training</h4>
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<h5>a).Knowing about the biological safety level and operation</h5>
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<h5>b).Personal protection</h5>
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<h5>c).Declined to away lab products</h5>
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<h4>1-1-2.Safe to use reagents and instruments</h4>
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<h5>a).Reagents:Knowing about the basic information and wearing gloves</h5>
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<h5>b).Instruments:Following the rules of use and power off in time</h5>
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<h4>1-1-3.The cleaning</h4>
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<p class="third">Keep the surroundings clean, be responsible for health and make things easy for others.</p>
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<h5>a).Timely cleaning test tubes, cuture dishes and etc.</h5>
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<h5>b).Taking out the garbage in time and marking fragile</h5>
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<h5>c).Air the room regularly</h5>
 +
    <a href="https://static.igem.org/mediawiki/2016/a/ae/NAU_CHINA_SAFETY_PDF.pdf"><p id="chaolianjie">Click here to download our safety training pdf.</p></a>
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<h3>1-2.Waste disposal</h3>
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<p class="second">Centralized treatment of waste disposal</p>
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<!-- 以上是Details的第一部分Lab safety -->
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<h2>2.Project safety</h2>
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<h3>a).Choosing a non-pathogenic chassis</h3>
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<table>
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<thead>
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<tr>
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<th>Species</th>
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<th>Risk Group</th>
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<th>Risk to human</th>
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<th>Function</th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td>E.Coli DH5α</td>
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<td>1</td>
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<td>Non pathogenic</td>
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<td>Amplified strain</td>
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</tr>
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<tr>
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<td>E.Coli BL21(DE3)</td>
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<td>1</td>
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<td>Non pathogenic</td>
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<td>Expression strain</td>
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</tr>
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<tr>
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<td>Sphingobium sp.YBL2</td>
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<td>1</td>
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<td>Cause inflammation</td>
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<td>Parent strain</td>
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</tr>
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<tr>
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<td>Klebsiella pneumoniae M5a1</td>
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<td>2</td>
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<td>Acute inflammation of lung</td>
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<td>Parent strain</td>
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</tr>
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<tr>
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<td>Sphingobium wenxiniae JZ-1</td>
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<td>1</td>
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<td>Cause inflammation</td>
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<td>Parent strain</td>
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</tr>
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</tbody>
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</table>
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<h3>b).Using physical-containment strategy to avoid modified organisms escaping</h3>
 +
<!-- 以上是Details的第二部分Project Safety,含表格-->
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 +
<h2>3.Social survey security</h2>
 +
<p class="second">This time our project referred to pesticide application status but it is a sensitive topic. Most relevant organizations were unwilling to give information even sometimes showed their anger. We have to keep ourselves safe in this circumstance. Stop asking the questions and leave in time.</p>
 +
 
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Revision as of 19:20, 14 October 2016


Pic.1 General Safety Matters

Details

1.Lab safety

1-1.Laboratory daily etiquette

No matter where we have to show repect to each other. Like removing the glove from your hand when you open the door avoiding staining it. Many details have to be amphasised but hard to list.

1-1-1. Safety training

a).Knowing about the biological safety level and operation
b).Personal protection
c).Declined to away lab products

1-1-2.Safe to use reagents and instruments

a).Reagents:Knowing about the basic information and wearing gloves
b).Instruments:Following the rules of use and power off in time

1-1-3.The cleaning

Keep the surroundings clean, be responsible for health and make things easy for others.

a).Timely cleaning test tubes, cuture dishes and etc.
b).Taking out the garbage in time and marking fragile
c).Air the room regularly

Click here to download our safety training pdf.

1-2.Waste disposal

Centralized treatment of waste disposal

2.Project safety

a).Choosing a non-pathogenic chassis

Species Risk Group Risk to human Function
E.Coli DH5α 1 Non pathogenic Amplified strain
E.Coli BL21(DE3) 1 Non pathogenic Expression strain
Sphingobium sp.YBL2 1 Cause inflammation Parent strain
Klebsiella pneumoniae M5a1 2 Acute inflammation of lung Parent strain
Sphingobium wenxiniae JZ-1 1 Cause inflammation Parent strain

b).Using physical-containment strategy to avoid modified organisms escaping

3.Social survey security

This time our project referred to pesticide application status but it is a sensitive topic. Most relevant organizations were unwilling to give information even sometimes showed their anger. We have to keep ourselves safe in this circumstance. Stop asking the questions and leave in time.