Difference between revisions of "Team:KoreaSonyeodul/Design"

 
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       .site {width: 100%}
 
       .site {width: 100%}
 
       .site h1 {
 
       .site h1 {
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       margin-top: 250px;
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       margin-top: 15%;
 
       color: #ffffff;
 
       color: #ffffff;
 
       text-decoration: none;
 
       text-decoration: none;
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       font-size: 80px;
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       font-size: 800%;
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       height: 35px;
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       height: 35px; <!--error-->
 
       background-color: rgba(255, 255, 255, 0.0);}
 
       background-color: rgba(255, 255, 255, 0.0);}
 
       .site h2 {margin-top: 0; position: relative; margin-bottom: 300px; padding-bottom: 100px;
 
       .site h2 {margin-top: 0; position: relative; margin-bottom: 300px; padding-bottom: 100px;
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       font-size: 50px;
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       font-size: 300%;
 
       color: #ffffff;
 
       color: #ffffff;
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       height: 30px;
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font-weight: 100;
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       height: 30px;<!--error-->
 
       }
 
       }
 
       table {text-align: center;}
 
       table {text-align: center;}
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       }
 
       }
 
.content1 h2{ font-weight: normal;
 
.content1 h2{ font-weight: normal;
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font-size: 25px;
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line-height: 1.3em;
 
}
 
}
 
     </style>
 
     </style>
 
     <link href="http://netdna.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.css" rel="stylesheet"></link>
 
     <link href="http://netdna.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.css" rel="stylesheet"></link>
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     <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
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     <link href="https://fonts.googleapis.com/css?family=Roboto:100,300" rel="stylesheet">
 
       <meta name= "viewport" content = "initial-scale=1.0, width= device-width" />
 
       <meta name= "viewport" content = "initial-scale=1.0, width= device-width" />
 
     </link>
 
     </link>
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           </h1>
 
           </h1>
 
           <h2>
 
           <h2>
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             <font face="Roboto">Main Subtitle</font>
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             <font face="Roboto">Design of the Project</font>
 
           </h2>
 
           </h2>
 
         </div>
 
         </div>
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         <div class="content1">
 
         <div class="content1">
 
           <h2>
 
           <h2>
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            The e-coli bacteria, DH+alpha, which is already located inside the mealworm's gut has the ability to decompose polystryene. However, polystryene only takes 7.8% while PET is more than 51%. Unfortunately, the mealworms don’t have the ability to decome polyehtylene yet. However, on the bright side, there is already a DNA artificially made by scientists that could decompose PET: the PUC 19. PUC 19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. So what we are trying to do is recombine DH+alpha and PUC19 by chemical transforming them.
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The e-coli, DH5alpha, which is already located inside the mealworm's gut has the ability to decompose polystyrene. However, polystyrene only takes 7.8% of world plastic waste, while PET takes more than 51%. Unfortunately, the mealworms did not have the ability to decompose polyethylene just yet. On the bright side however, we discovered an enzyme that was artificially made by scientists to decompose PET: PETase. In our project we tried to recombine DH5alpha and PETase through chemical transformation and to see if the system worked. If they recombined properly, they will have resistance to the antibiotic in the LB Agar Medium. And that would be a sign that our PETase gene is safely incorporated in the e-coli’s DNA and that our system would work.  
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            If they are recombined properly, they would have the resistance to antibiotic which means that it would grow well in the agar badge. Now all we have to do is put this recombined DNA inside the DNA. From this experiment we are trying to further the mealworm’s ability to decompose plastic. If we succeed, we would provide a new solution to increasing amount of plastic.
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           </h2>
 
           </h2>
 
           </div>
 
           </div>

Latest revision as of 05:40, 16 October 2016

DESIGN

Design of the Project

The e-coli, DH5alpha, which is already located inside the mealworm's gut has the ability to decompose polystyrene. However, polystyrene only takes 7.8% of world plastic waste, while PET takes more than 51%. Unfortunately, the mealworms did not have the ability to decompose polyethylene just yet. On the bright side however, we discovered an enzyme that was artificially made by scientists to decompose PET: PETase. In our project we tried to recombine DH5alpha and PETase through chemical transformation and to see if the system worked. If they recombined properly, they will have resistance to the antibiotic in the LB Agar Medium. And that would be a sign that our PETase gene is safely incorporated in the e-coli’s DNA and that our system would work.