Difference between revisions of "Team:KoreaSonyeodul/Proof"

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{{KoreaSonyeodul}}
 
{{KoreaSonyeodul}}
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<html lang="en">
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  <head>
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    <style>
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      #wrap {width: 60%; margin: auto;}
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      body{
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      margin: 0;
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      background: url('https://static.igem.org/mediawiki/2016/e/ee/T--KoreaSonyeodul--TitleBackground.png');
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font-weight: 100;
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      table {text-align: center;}
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      .site .mainBox .mainBox1 {width: 1000px; margin-left: auto; margin-right: auto;}
  
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<html>
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      <!--FadeIn-->
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      @import url(http://fonts.googleapis.com/css?family=Open+Sans+Condensed:300);
  
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      body {padding: 0; margin: 0; background-color: #333;}
  
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<div class="column full_size judges-will-not-evaluate">
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      .container {position: fixed; top: 25%; left: 25%;}
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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      .fade-in {
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</div>
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  </head>
  
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<p>
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  <body>
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
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</p>
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    <!--Title-->
  
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<h4> What should we do for our proof of concept? </h4>
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    <div class="box fade-in one">
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<p>  
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      <div id="" align="center">
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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        <div id="fontlight" class="site">
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</p>
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          <h1>
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            <font face="Roboto" weight="200">Proof</font>
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          </h1>
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          <h2>
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            <font face="Roboto">Design of the Project</font>
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          </h2>
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        </div>
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      </div>
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    </div>
  
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    <div id="wrap">
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      <div class="box fade-in two">
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        <div class="content1">
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<h1>Proof of the Chemical Transformation of the e-coli</h1>
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          <h2>
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We used the LIC Method for cloning PETase and E-coli. The basic mechanism of cloning is simple: put gene A into plasmid or the target vector to make it work. With restriction enzyme, we cut the plasmid and put gene A into the part where it is cut out. From this on, the plasmid will be called a backbone. To fit in gene A, we have to make the ends of the cut backbone complementary with gene A. The method of annealing single stranded complementary is by using LIC (Ligation Independent Cloning). We use PCR for this process. Then, we mix gene A and the backbone and insert it in e.coli for transformation. This is how we put PETase into the backbone vector. A bacillus is used to put PETase on the surface to expose it to external surroundings. Our experiment has finished putting PETase and backbone in the e.coli, but we are in the process of verifying our results.
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          </h2>
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          </div>
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<div class="firstimg"><img src="https://static.igem.org/mediawiki/2016/1/15/T--KoreaSonyeodul--DesignExp.png"></img></div>
  
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        <div class="content1">
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<h1>Proof of the competent mealworm</h1>
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          <h2>
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After successfully cloning the PETase and MHETase into the E.coli, our team planned to feed that E.coli to the mealworms in order to add the ability of PET to these mealworms. We conducted this process of proof in three ways.
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First way is to use the qualitative method to simply check whether the mealworms could degrade the PET.
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Second way is to check whether mealworms could survive through only eating PET as their supply of nutrient. Third is to gather the quantitative data that specifically measures the speed and amount of PET being degraded. This method is similar to those utilized during the experiment checking the speed of polystyrene degradation rate of mealworms.
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We are currently in the process of carrying out these three experiments.
  
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</div>
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          </h2>
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          </div>
  
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        </div>
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      <div class="box fade-in three">
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      </div>
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      <!--Footer-->
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    </body>
 
</html>
 
</html>

Latest revision as of 05:46, 16 October 2016

Proof

Design of the Project

Proof of the Chemical Transformation of the e-coli

We used the LIC Method for cloning PETase and E-coli. The basic mechanism of cloning is simple: put gene A into plasmid or the target vector to make it work. With restriction enzyme, we cut the plasmid and put gene A into the part where it is cut out. From this on, the plasmid will be called a backbone. To fit in gene A, we have to make the ends of the cut backbone complementary with gene A. The method of annealing single stranded complementary is by using LIC (Ligation Independent Cloning). We use PCR for this process. Then, we mix gene A and the backbone and insert it in e.coli for transformation. This is how we put PETase into the backbone vector. A bacillus is used to put PETase on the surface to expose it to external surroundings. Our experiment has finished putting PETase and backbone in the e.coli, but we are in the process of verifying our results.

Proof of the competent mealworm

After successfully cloning the PETase and MHETase into the E.coli, our team planned to feed that E.coli to the mealworms in order to add the ability of PET to these mealworms. We conducted this process of proof in three ways. First way is to use the qualitative method to simply check whether the mealworms could degrade the PET. Second way is to check whether mealworms could survive through only eating PET as their supply of nutrient. Third is to gather the quantitative data that specifically measures the speed and amount of PET being degraded. This method is similar to those utilized during the experiment checking the speed of polystyrene degradation rate of mealworms. We are currently in the process of carrying out these three experiments.