Difference between revisions of "Team:KoreaSonyeodul/Parts"

Line 114: Line 114:
 
<h2>Creator</h2> <h3>Seung-Woo Baek</h3>
 
<h2>Creator</h2> <h3>Seung-Woo Baek</h3>
 
<h2>Sequence</h2>
 
<h2>Sequence</h2>
−
<h3><br>ATGAACTTTCCTCGCGCCTCCCGCTTGATGCAAGCAGCAGTCCTGGGAGGTTTAATGGCGGTCAGCGCTGCGGCGACGGCACAGACGAATCCTTACGCTCGTGGTCCAAACCCGACGGCAGCCAGTTTGGAAGCA
+
<h3><br>ATGAACTTTCCTCGCGCCTCCCGCTTGATGCAAGCAGCAGTCCTGGGAGGTTTAATGGCGGTCAGCGCTGCGGCGACGGCACAGACGAATCCTTACGCTCGTGGTCCAAACCCGACGGCAGCCAGTTTGGAAGC
−
TCGGCTGGGCCTTTCACTGTCCGCAGCTTTACTGTTTCCCGCCCCTCTGGATATGGCGCAGGGACTGTCTATTACCCGACCAACGCGGGGGGCACGGTAGGAGCTATTGCAATCGTCCCCGGATACACTGCACGTCAGT
+
ATCGGCTGGGCCTTTCACTGTCCGCAGCTTTACTGTTTCCCGCCCCTCTGGATATGGCGCAGGGACTGTCTATTACCCGACCAACGCGGGGGGCACGGTAGGAGCTATTGCAATCGTCCCCGGATACACTGCACGTCA
−
CATCCATCAAGTGGTGGGGACCTCGTTTGGCCAGTCACGGTTTTGTCGTGATTACCATTGACACCAACTCAACCTTGGATCAGCCGAGCTCCCGTTCAAGTCAACAGATGGCCGCGTTGCGTCAGGTAGCCAGCCTGAA
+
GTCATCCATCAAGTGGTGGGGACCTCGTTTGGCCAGTCACGGTTTTGTCGTGATTACCATTGACACCAACTCAACCTTGGATCAGCCGAGCTCCCGTTCAAGTCAACAGATGGCCGCGTTGCGTCAGGTAGCCAGCCT
−
CGGCACGAGCAGTTCACCTATCTATGGCAAAGTGGACACCGCGCGCATGGGAGTCATGGGCTGGTCTATGGGCGGGGGTGGAAGTCTGATTAGCGCGGCGAATAACCCCAGCTTAAAGGCAGCTGCGCCTCAAGCCCCT
+
GAACGGCACGAGCAGTTCACCTATCTATGGCAAAGTGGACACCGCGCGCATGGGAGTCATGGGCTGGTCTATGGGCGGGGGTGGAAGTCTGATTAGCGCGGCGAATAACCCCAGCTTAAAGGCAGCTGCGCCTCAAGC
−
TGGGACTCGTCCACGAATTTCTCTTCTGTCACCGTACCCACCCTTATTTTTGCTTGCGAAAATGATTCCATCGCCCCGGTAAATTCATCGGCGTTACCAATTTATGACTCGATGAGCCGCAACGCCAAGCAATTCCTGG
+
CCCTTGGGACTCGTCCACGAATTTCTCTTCTGTCACCGTACCCACCCTTATTTTTGCTTGCGAAAATGATTCCATCGCCCCGGTAAATTCATCGGCGTTACCAATTTATGACTCGATGAGCCGCAACGCCAAGCAATT
−
AGATTAATGGGGGGAGTCATTCGTGTGCAAATAGTGGAAACTCCAACCAGGCATTAATCGGCAAAAAGGGGGTAGCTTGGATGAAGCGTTTCATGGACAACGACACGCGTTACTCAACTTTCGCCTGTGAAAACCCAAA
+
CCTGGAGATTAATGGGGGGAGTCATTCGTGTGCAAATAGTGGAAACTCCAACCAGGCATTAATCGGCAAAAAGGGGGTAGCTTGGATGAAGCGTTTCATGGACAACGACACGCGTTACTCAACTTTCGCCTGTGAAAA
−
TTCCACGCGCGTAAGCGATTTCCGTACCGCAAATTGCTCCTAA</h3>
+
CCCAAATTCCACGCGCGTAAGCGATTTCCGTACCGCAAATTGCTCCTAA</h3>
 
<h2>Short description</h2>
 
<h2>Short description</h2>
 
<h3>Forms PETase which degrades PET.</h3>
 
<h3>Forms PETase which degrades PET.</h3>

Revision as of 06:10, 16 October 2016

Parts

Design of the Project

Part name

BBa_K1999000 (PETase)

Part type

Protein coding sequence

Creator

Seung-Woo Baek

Sequence


ATGAACTTTCCTCGCGCCTCCCGCTTGATGCAAGCAGCAGTCCTGGGAGGTTTAATGGCGGTCAGCGCTGCGGCGACGGCACAGACGAATCCTTACGCTCGTGGTCCAAACCCGACGGCAGCCAGTTTGGAAGC ATCGGCTGGGCCTTTCACTGTCCGCAGCTTTACTGTTTCCCGCCCCTCTGGATATGGCGCAGGGACTGTCTATTACCCGACCAACGCGGGGGGCACGGTAGGAGCTATTGCAATCGTCCCCGGATACACTGCACGTCA GTCATCCATCAAGTGGTGGGGACCTCGTTTGGCCAGTCACGGTTTTGTCGTGATTACCATTGACACCAACTCAACCTTGGATCAGCCGAGCTCCCGTTCAAGTCAACAGATGGCCGCGTTGCGTCAGGTAGCCAGCCT GAACGGCACGAGCAGTTCACCTATCTATGGCAAAGTGGACACCGCGCGCATGGGAGTCATGGGCTGGTCTATGGGCGGGGGTGGAAGTCTGATTAGCGCGGCGAATAACCCCAGCTTAAAGGCAGCTGCGCCTCAAGC CCCTTGGGACTCGTCCACGAATTTCTCTTCTGTCACCGTACCCACCCTTATTTTTGCTTGCGAAAATGATTCCATCGCCCCGGTAAATTCATCGGCGTTACCAATTTATGACTCGATGAGCCGCAACGCCAAGCAATT CCTGGAGATTAATGGGGGGAGTCATTCGTGTGCAAATAGTGGAAACTCCAACCAGGCATTAATCGGCAAAAAGGGGGTAGCTTGGATGAAGCGTTTCATGGACAACGACACGCGTTACTCAACTTTCGCCTGTGAAAA CCCAAATTCCACGCGCGTAAGCGATTTCCGTACCGCAAATTGCTCCTAA

Short description

Forms PETase which degrades PET.

Long description

Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Is also able to hydrolyze bis(hydroxyethyl) terephthalate (BHET) to yield MHET with no further decomposition. Shows low activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) in vitro