Difference between revisions of "Team:Stanford-Brown/SB16 Notebooks Interlab"

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<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">iGEM (InterLab, Cell Competency Kit, BioBrick Characterizations, etc.)</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> iGEM 2016</div><div><b>Authors:</b> Taylor Sihavong, Charles Gleason, Michael Becich</div><div><b>Dates:</b> 2016-06-16 to 2016-09-06</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/16/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3452" data-index="0" style="margin-left:0;">-Cell competency test done on 3 different DNA concentrations (0.5pg/ul, 10pg/ul, 50pg/ul) with 2 different cell types (T7, 5-alpha)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3453" data-index="1" style="margin-left:35px;">-Protocol given for the competency test wasn't compatible with T7, but we got colonies on all of our 5-alpha plates (Charlie, Taylor)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3454" data-index="2" style="margin-left:0;">-Began InterLab study. Started with the high efficiency transformation protocol for NEB 5-alpha Competent <i>E. Coli. </i>Transformed 5 DNA samples: positive control, negative control, and 3 test devices. (Taylor, Cynthia, Amy)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3455" data-index="3" style="margin-left:35px;">-Test device 3 was diluted with 100ul DI water with 5ul of DNA taken out</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3456" data-index="4" style="margin-left:35px;">-Plated with 1:4 dilutions</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3457" data-index="5" style="margin-left:0;">-InterLab study results: test device 1 plate had no colonies --&gt; store positive control, negative control, test device 2, test device 3 at -4˚C (Charlie)</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part3458-item" data-formattable="true" data-index="6" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_2671.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="eWGAcjF3RzslqdqGC33LBDbTWVF9xBB5Hz5KdEDS-IMG_2671.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part3458" data-index="6">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3460" data-index="7" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3461" data-index="8" style="margin-left:0;">-Redo transformation of test device 1 and test device 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3462" data-index="9" style="margin-left:35px;">-Test device 1 was diluted with 100ul DI water with 5ul of DNA taken out</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3463" data-index="10" style="margin-left:35px;">-2 plates each, a 4X dilution and a 40X dilution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3464" data-index="11" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3465" data-index="12" style="margin-left:35px;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3466" data-index="13" style="margin-left:0;">-Team Brainstorming Session: Where are we at and where are we heading?</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3467" data-index="14" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part3468-item" data-formattable="true" data-index="15" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:648px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_2634.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Z5H9Gm5CoIdUpbV8d4lQlZeto8syTY9x8TytQsQ3-IMG_2634.JPG.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable 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</div></div><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3470" data-index="16" style="margin-left:0;">-Performed calibration plate readings (2 plates) for interlab study (Taylor S)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3471" data-index="17" style="margin-left:0;">-Prepared overnight cultures for interlab study (2 colonies per plate, 5 plates) (Mike)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3472" data-index="18" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3473" data-index="19" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">-Made Terrific Broth (TB, 0.17M KH<sub>2</sub>PO<sub>4</sub>, 0.72M K<sub>2</sub>HPO<sub>4</sub>) buffer for interlab study, Prepared Interlab Samples for Weekend Storage</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3474" data-index="20" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3475" data-index="21" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">- Worked on part 2 of Interlab Measurement Study for 6 hours, measured fluorescence after 6 hours with SpectraMax Plus 384</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3476" data-index="22" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3477" data-index="23" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3478" data-index="24" style="margin-left:35px;">37th Anniversary of Putting First Human on the Moon!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3479" data-index="25" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3480" data-index="26" style="margin-left:35px;">NASA Star Trek Premiere Special Showing!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3481" data-index="27" style="margin-left:35px;">Jim Head Presentation and Q&amp;A (Mars Climate Change, Psychology of Family of Planets)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3482" data-index="28" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3483" data-index="29" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3484" data-index="30" style="margin-left:0;">-Hackathon to learn methods for improving website</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3485" data-index="31" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3486" data-index="32" style="margin-left:35px;">Freeze dried 10 uL of first 8 BioBricks!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part3487" data-index="33" style="margin-left:70px;">1st Submission: rAIP, AE Blue, 1 np Et?, Gb2, c1, c2, c3, cw</div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div>
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Revision as of 02:36, 17 October 2016


Stanford-Brown 2016

iGEM (InterLab, Cell Competency Kit, BioBrick Characterizations, etc.) · Benchling

iGEM (InterLab, Cell Competency Kit, BioBrick Characterizations, etc.)

Made with Benchling
Project: iGEM 2016
Authors: Taylor Sihavong, Charles Gleason, Michael Becich
Dates: 2016-06-16 to 2016-09-06
Thursday, 6/16/16
-Cell competency test done on 3 different DNA concentrations (0.5pg/ul, 10pg/ul, 50pg/ul) with 2 different cell types (T7, 5-alpha)
-Protocol given for the competency test wasn't compatible with T7, but we got colonies on all of our 5-alpha plates (Charlie, Taylor)
Monday, 6/20
-Began InterLab study. Started with the high efficiency transformation protocol for NEB 5-alpha Competent E. Coli. Transformed 5 DNA samples: positive control, negative control, and 3 test devices. (Taylor, Cynthia, Amy)
-Test device 3 was diluted with 100ul DI water with 5ul of DNA taken out
-Plated with 1:4 dilutions
Tuesday, 6/21
-InterLab study results: test device 1 plate had no colonies --> store positive control, negative control, test device 2, test device 3 at -4˚C (Charlie)
IMG_2671.JPG
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-Redo transformation of test device 1 and test device 3
-Test device 1 was diluted with 100ul DI water with 5ul of DNA taken out
-2 plates each, a 4X dilution and a 40X dilution
Tuesday, 6/28
-Team Brainstorming Session: Where are we at and where are we heading?
IMG_2634.JPG
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Thursday, 6/30
-Performed calibration plate readings (2 plates) for interlab study (Taylor S)
-Prepared overnight cultures for interlab study (2 colonies per plate, 5 plates) (Mike)
Friday, 7/1
-Made Terrific Broth (TB, 0.17M KH2PO4, 0.72M K2HPO4) buffer for interlab study, Prepared Interlab Samples for Weekend Storage
Tuesday, 7/5
- Worked on part 2 of Interlab Measurement Study for 6 hours, measured fluorescence after 6 hours with SpectraMax Plus 384
Wednesday, 7/20
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37th Anniversary of Putting First Human on the Moon!
Thursday, 7/21
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NASA Star Trek Premiere Special Showing!
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Jim Head Presentation and Q&A (Mars Climate Change, Psychology of Family of Planets)
Friday, 7/22
Saturday, 7/23
-Hackathon to learn methods for improving website
Tuesday, 9/6
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Freeze dried 10 uL of first 8 BioBricks!
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1st Submission: rAIP, AE Blue, 1 np Et?, Gb2, c1, c2, c3, cw