Difference between revisions of "Team:Pittsburgh/Notebook"

Line 5: Line 5:
 
<div class="column full_size">
 
<div class="column full_size">
  
<p>Our weekly progress</p>
+
<p>Our weekly progress. For a list of our protocols, visit the <a href="https://2016.igem.org/Team:Pittsburgh/Protocols" target="_blank"> Protocols</a> page</p>
  
 
</div>
 
</div>

Revision as of 16:56, 5 July 2016

Our weekly progress. For a list of our protocols, visit the Protocols page

Week 1: May 23 - May 27

Wet Lab

Dry Lab

  • Brainstorm genetic circuits for a thallium sensor
  • Lab safety training
Week 1 Notebook
Back to Top

Week 2: May 31 - June 3

Wet Lab

Cell-Free Extract

Dry Lab

  • Contact museums and summer programs for outreach opportunities
  • Lab safety training
Week 2 Notebook
Back to Top

Week 3: June 6 - June 12

Wet Lab

Reporter

  • Transform T7 promoter, amilCP, and terminator
  • Begin assembly by ligating linearized T7 promoter and amilCP

Dry Lab

  • Contact museums and summer programs for outreach opportunities
Week 3 Notebook
Back to Top

Week 4: June 13 - June 17

Wet Lab

Reporter

  • Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
  • Send promising T7 promoter -- amilCP ligations to be sequenced
  • Perform double digest of T7 promoter and terminator from last week
  • Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)

Dry Lab

  • TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
Week 4 Notebook
Back to Top

Week 5: June 20 - June 26

Wet Lab

Reporter

  • Ligate T7 promoter -- amilCP construct to terminator
  • Extract successful ligations of T7 promoter to eGFP
  • Ligate T7 promoter -- eGFP construct to terminator

Cell-Free Extract

  • Reaction volume can be reduced to 5 μL with 5 ng/μL of DNA

Toehold Switch

  • Collins triggers activate the switches (both in plasmid form) to express LacZ

Dry Lab

  • Reach out to teams to collaborate based on last year's projects
Week 5 Notebook
Back to Top

Week 6: June 27 - July 3

Wet Lab

Reporter

  • Identify successful ligations to terminator for amilCP and eGFP consturcts using a gel
  • Send correct plasmids for sequencing for confirmation
  • Test plasmids in cell-free extract
  • amilCP does not produce color in cell-free reaction
  • eGFP produces fluorescence comparable to that from the Collins T7-GFP plasmid in cell-free reaction

Cell-Free Extract

  • Reaction volume can be reduced to 1 μL with 5 ng/μL of DNA

Toehold Switch

  • Collins plasmids express LacZ with 25 ng of switch
  • DNA oligos trigger Collins switches

Dry Lab

  • Work on outreach presentation for tissue engineering camp
Week 6 Notebook
Back to Top