Difference between revisions of "Team:Pittsburgh/Description"

 
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    <p>Our improved characterization of codon-optimized fluorescent proteins developed by the <a href="https://2015.igem.org/Team:Carnegie_Mellon/Parts" target="_blank">2015 Carnegie Mellon</a> team.</p>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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    <p>We characterized the proteins as possible reporters for our system. However, we decided not to use fluorescent proteins since they cannot be seen with the naked eye unless they are in high concentration. We added fluorescence and kinetics characterization to the following parts:</p>
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<h5>Advice on writing your Project Description</h5>
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    <li>YFP, <a href="http://parts.igem.org/Part:BBa_K1732012" target="_blank">Part BBa_K1732012</a></li>
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    <li>OFP, <a href="http://parts.igem.org/Part:BBa_K1732011" target="_blank">Part BBa_K1732011</a></li>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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    <li>BFP, <a href="http://parts.igem.org/Part:BBa_K1732007" target="_blank">Part BBa_K1732007</a></li>
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    <li>GFP, <a href="http://parts.igem.org/Part:BBa_K1732009" target="_blank">Part BBa_K1732009</a></li>  
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    <li>RFP, <a href="http://parts.igem.org/Part:BBa_K1732013" target="_blank">Part BBa_K1732013</a></li>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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    <p>All readings were performed on the Tecan M1000. All excitation and emission peaks were close to the reported values except the excitation of GFP. Most proteins (except GFP and OFP) reached maximum fluorescence within 6 hours of growth.
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Latest revision as of 21:28, 18 October 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

Our improved characterization of codon-optimized fluorescent proteins developed by the 2015 Carnegie Mellon team.

We characterized the proteins as possible reporters for our system. However, we decided not to use fluorescent proteins since they cannot be seen with the naked eye unless they are in high concentration. We added fluorescence and kinetics characterization to the following parts:

All readings were performed on the Tecan M1000. All excitation and emission peaks were close to the reported values except the excitation of GFP. Most proteins (except GFP and OFP) reached maximum fluorescence within 6 hours of growth.