Difference between revisions of "Team:Pittsburgh/Description"

 
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     <p>We added fluorescence scans and kinetics information to the following parts:
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     <p>We characterized the proteins as possible reporters for our system. However, we decided not to use fluorescent proteins since they cannot be seen with the naked eye unless they are in high concentration. We added fluorescence and kinetics characterization to the following parts:</p>
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     <ul>
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     <li>YFP, <a href="http://parts.igem.org/Part:BBa_K1732012" target="_blank">Part BBa_K1732012</a></li>
 
     <li>YFP, <a href="http://parts.igem.org/Part:BBa_K1732012" target="_blank">Part BBa_K1732012</a></li>
 
     <li>OFP, <a href="http://parts.igem.org/Part:BBa_K1732011" target="_blank">Part BBa_K1732011</a></li>
 
     <li>OFP, <a href="http://parts.igem.org/Part:BBa_K1732011" target="_blank">Part BBa_K1732011</a></li>
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     <li>RFP, <a href="http://parts.igem.org/Part:BBa_K1732013" target="_blank">Part BBa_K1732013</a></li>
 
     <li>RFP, <a href="http://parts.igem.org/Part:BBa_K1732013" target="_blank">Part BBa_K1732013</a></li>
 
     </ul>
 
     </ul>
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     All readings were performed on the Tecan M1000.
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     <p>All readings were performed on the Tecan M1000. All excitation and emission peaks were close to the reported values except the excitation of GFP. Most proteins (except GFP and OFP) reached maximum fluorescence within 6 hours of growth.
 
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Latest revision as of 21:28, 18 October 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

Our improved characterization of codon-optimized fluorescent proteins developed by the 2015 Carnegie Mellon team.

We characterized the proteins as possible reporters for our system. However, we decided not to use fluorescent proteins since they cannot be seen with the naked eye unless they are in high concentration. We added fluorescence and kinetics characterization to the following parts:

All readings were performed on the Tecan M1000. All excitation and emission peaks were close to the reported values except the excitation of GFP. Most proteins (except GFP and OFP) reached maximum fluorescence within 6 hours of growth.