Difference between revisions of "Team:Pittsburgh/Parts"

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{{Pittsburgh}}
 
{{Pittsburgh}}
 
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         <p>The Parts we developed</p></div>
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         <p>The Parts we used to build our circuit.</p></div>
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             <tr><br><br><br>
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                   <td><h3><b>PART NAME</b></h3></td>
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        <p>The Parts Pitt iGEM developed over the summer are listed below. Take a look by following the provided links.</p>
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                   <td><h3><b>PART NUMBER</b></h3></td>
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         <table align=center class="parts">
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             <tr>
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                  <td style="width:30%;"><h3><b>Part Name</b></h3></td>
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                   <td><h3><b>Part Description</b></h3></td>
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                   <td style="width:20%;"><h3><b>Part Number</b></h3></td>
  
 
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                   <td>T3 Promoter</td>
 
                   <td>T3 Promoter</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084000"> BBa_K2084000 </a></td>
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                <td>Promoter for the T3 RNA Polymerase</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084000" target="_blank"> BBa_K2084000 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
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                   <td>T3 RNA Polymerase</td>
 
                   <td>T3 RNA Polymerase</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084001"> BBa_K2084001 </a></td>
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                <td>Encodes the T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084001" target="_blank"> BBa_K2084001 </a></td>
 
             </tr>
 
             </tr>
  
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                   <td>pT7 Toehold </td>
 
                   <td>pT7 Toehold </td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084002"> BBa_K2084002 </a></td>
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                <td>Regulatory ribosome binding site with T7 RNAP promoter</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084002" target="_blank"> BBa_K2084002 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
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                   <td>pT7 Regulated T3 RNA Polymerase</td>
 
                   <td>pT7 Regulated T3 RNA Polymerase</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084003"> BBa_K2084003 </a></td>
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                <td>pT7-Toehold, T3 RNAP, and terminator for controlling and regulating sequences with a T3 RNAP promoter</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084003" target="_blank"> BBa_K2084003 </a></td>
 
             </tr>
 
             </tr>
  
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                   <td>pT3 with RBS</td>
 
                   <td>pT3 with RBS</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084004"> BBa_K2084004 </a></td>
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                <td>T3 promoter with an RBS site added</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084004" target="_blank"> BBa_K2084004 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT7 Toehold Regulated LacZ</td>
 
                   <td>pT7 Toehold Regulated LacZ</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084005"> BBa_K2084005 </a></td>
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                <td>BBa_K2084002 with a mutagenized <i>lacZ</i></td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084005" target="_blank"> BBa_K2084005 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT3 Controlled GFP</td>
 
                   <td>pT3 Controlled GFP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084006"> BBa_K2084006 </a></td>
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                <td>T3 RNAP promoter site to translate GFP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084006" target="_blank"> BBa_K2084006 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT7 Controlled T3 RNAP</td>
 
                   <td>pT7 Controlled T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084007"> BBa_K2084007 </a></td>
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                <td>Translated by T7 RNAP to create T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084007" target="_blank"> BBa_K2084007 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>T3 RNA Polymerase Amplifier</td>
 
                   <td>T3 RNA Polymerase Amplifier</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084008"> BBa_K2084008 </a></td>
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                <td>T3 promoter and gene</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084008" target="_blank"> BBa_K2084008 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
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                   <td>pT3 Controlled LacZ</td>
 
                   <td>pT3 Controlled LacZ</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084009"> BBa_K2084009 </a></td>
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                <td>LacZ reporter regulated by T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084009" target="_blank"> BBa_K2084009 </a></td>
 
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    <!--moved to Attributions
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    <h2>Thanks to:</h2>
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          <p> Pitt iGEM would like to thank the Collins Laboratory at MIT for the pT7 toehold structures that they kindly sent us. We would also like to thank the Voigt Laboratory at MIT for the pT3 controlled GFP construct.</p>-->
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<h5>Note</h5>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h5>Adding parts to the registry</h5>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h5>Inspiration</h5>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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Latest revision as of 00:20, 20 October 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

The Parts we used to build our circuit.

The Parts Pitt iGEM developed over the summer are listed below. Take a look by following the provided links.

Part Name

Part Description

Part Number
T3 Promoter Promoter for the T3 RNA Polymerase BBa_K2084000
T3 RNA Polymerase Encodes the T3 RNAP BBa_K2084001
pT7 Toehold Regulatory ribosome binding site with T7 RNAP promoter BBa_K2084002
pT7 Regulated T3 RNA Polymerase pT7-Toehold, T3 RNAP, and terminator for controlling and regulating sequences with a T3 RNAP promoter BBa_K2084003
pT3 with RBS T3 promoter with an RBS site added BBa_K2084004
pT7 Toehold Regulated LacZ BBa_K2084002 with a mutagenized lacZ BBa_K2084005
pT3 Controlled GFP T3 RNAP promoter site to translate GFP BBa_K2084006
pT7 Controlled T3 RNAP Translated by T7 RNAP to create T3 RNAP BBa_K2084007
T3 RNA Polymerase Amplifier T3 promoter and gene BBa_K2084008
pT3 Controlled LacZ LacZ reporter regulated by T3 RNAP BBa_K2084009