Difference between revisions of "Team:Pittsburgh/Experiments"
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| − | <p>The main experiments we performed to develop our sensor</p></div> | + | <p>The main experiments we performed to develop our sensor. For our daily activities and experiments, visit our <a href="2016.igem.org/Team:Pittsburgh/Notebook" target="_blank">Notebook</a>. For a list of our protocols, check out our <a href="2016.igem.org/Team:Pittsburgh/Protocols" target="_blank">Protocols</a> page.</p></div> |
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Revision as of 17:46, 2 August 2016
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The main experiments we performed to develop our sensor. For our daily activities and experiments, visit our Notebook. For a list of our protocols, check out our Protocols page.
Contents
Reporter
amilCP
amilCP is a chromoprotein added to the iGEM Registry by Uppsala Sweden, 2012. We were unable to produce amilCP in bacteria or in our cell-free system.
Back to TopCell-Free System
Linear versus Plasmid DNA
We have good data from adding plasmids to our cell-free system. However, because the Collins paper specified the use of linear DNA in their cell-free systems, we compared the signal from GFP from linear and plasmid DNA. There is no siginificant difference between the two constructs.
dilution
volume optimization
Back to TopDNAzyme
Back to TopToehold Switch
Our experiments were performed with the D and G LacZ switches from the paper from the Collins group, "Paper-Based Synthetic Gene Networks".
RNA Trigger
In Week 5, we showed that LacZ is expressed when the plasmids for the switch and trigger constructs are combined in a cell-free system.
DNA Trigger
In Week 6, we showed that toehold switches are activated by single-stranded DNA triggers, but activation levels are lower than with RNA triggers.
Sequestered DNA Trigger
In Week 7, we showed that single-stranded DNA triggers do not activate toehold switches if they are part of a hybridized complex.
Back to TopSignal Amplification System
Back to TopDifferential Amplifier
Back to Top