Difference between revisions of "Team:Pittsburgh/Project Overview"
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Revision as of 02:58, 4 September 2016
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Learn how Hot Metal Switch works!
Contents
- Hot Metal Switch
- In Vitro Protein Synthesis
- Genetic Circuit
DNAzyme
Toehold Switch
Reporter
AmplifierHot Metal Switch
Hot Metal Switch is a cell-free sensor that conditionally expresses lacZ only in the presence of the target metal, either lead or thallium. The metal ion detection is based on specific DNAzymes that are cleaved in the presence of their target metal. The released DNA strand activates a toehold switch regulated by the T7 promoter system, which will promote transcription of T3 RNA polymerase. The T3 RNA polymerase will activate the expression of lacZ, which will produce a color change visible to the naked eye. The T3 RNA polymerase will also activate additional expression of itself to amplify the original signal from the DNAzyme cleavage.
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In Vitro Protein Synthesis
Back to TopGenetic Circuit
Back to TopDNAzyme
Back to TopToehold Switch
Back to TopReporter
Back to TopAmplifier
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