Difference between revisions of "Team:Pittsburgh/Notebook"
(add week 4) |
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<ul> | <ul> | ||
<li>Transform T7 promoter, amilCP, and terminator</li> | <li>Transform T7 promoter, amilCP, and terminator</li> | ||
| − | <li>Begin assembly by ligating T7 promoter and amilCP</li> | + | <li>Begin assembly by ligating linearized T7 promoter and amilCP</li> |
</ul> | </ul> | ||
<h2>Dry Lab</h2> | <h2>Dry Lab</h2> | ||
| Line 65: | Line 65: | ||
<ul> | <ul> | ||
<li>Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP</li> | <li>Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP</li> | ||
| − | <li> | + | <li>Send promising T7 promoter -- amilCP ligations to be sequenced</li> |
| + | <li>Perform double digest of T7 promoter and terminator from last week</li> | ||
| + | <li>Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)</li> | ||
</ul> | </ul> | ||
<h2>Dry Lab</h2> | <h2>Dry Lab</h2> | ||
Revision as of 19:20, 17 June 2016
Contact Us
Thank you to our sponsors!
Our weekly progress
Week 1: May 23 - May 27
Wet Lab
- Training begins
- Grow Top 10 competent cells.
Dry Lab
- Brainstorm genetic circuits for a thallium sensor
- Lab safety training
Week 2: May 31 - June 3
Wet Lab
- Test efficiency of competent cells
- Test cell free extract reaction with T7-GFP plasmid
Dry Lab
- Contact museums and summer programs for outreach opportunities
- Lab safety training
Week 3: June 6 - June 12
Wet Lab
- Transform T7 promoter, amilCP, and terminator
- Begin assembly by ligating linearized T7 promoter and amilCP
Dry Lab
- Contact museums and summer programs for outreach opportunities
Week 4: June 13 - June 17
Wet Lab
- Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
- Send promising T7 promoter -- amilCP ligations to be sequenced
- Perform double digest of T7 promoter and terminator from last week
- Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)
Dry Lab
- TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set
