Revision as of 02:31, 17 October 2016

Stanford-Brown 2016

Gas Production · Benchling

Gas Production

Made with Benchling

Project: iGEM 2016

Authors: Taylor Sihavong

Dates: 2016-08-23 to 2016-09-23

Tuesday, 8/23

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Chlamydomonas reinhardtii + Soilwater: GR+ Medium + Soil Extract Medium came in the mail

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3 glass tubes prepared for algae growth in ~5ml liquid culture

●

Growth conditions: 20˚C, light from cool-white fluorescent lamps, 3200 lux maximum intensity, 12/12 hr light/dark

●

1 tube set at 20˚C in light incubator, 2 set at room temp

Thursday, 8/25

●

Tubes at room temp didn't grow, tube in light incubator had thin layer of alga growing on top of media. All tubes (including original agar tube) set in light incubator at ~21 W/m²

Thursday, 9/1

●

500ul taken from one of three tubes in the light incubator and put in a flask with BG-11

○

Media lacks sulfur; is a test for hydrogen production capabilities

Monday, 9/12

●

Checked flask, full of growth! All good. Gas production should be good to go

Tuesday, 9/20

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Flask with BG-11 prepared with water balloon and nozzle up top, left overnight to see if gas production occurred and balloon inflated

Friday, 9/23

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Flask is moved to Stanford labs for further testing

●

When gas in flask is introduced to flame, a pop is heard characteristic of hydrogen gas

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 <body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Gas Production</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> iGEM 2016</div><div><b>Authors:</b> Taylor Sihavong</div><div><b>Dates:</b> 2016-08-23 to 2016-09-23</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part999" data-index="0" style="margin-left:35px;"><i>Chlamydomonas reinhardtii</i> + Soilwater: GR+ Medium + Soil Extract Medium came in the mail</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1000" data-index="1" style="margin-left:35px;">3 glass tubes prepared for algae growth in ~5ml liquid culture</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1001" data-index="2" style="margin-left:35px;">Growth conditions: 20˚C, light from cool-white fluorescent lamps, 3200 lux maximum intensity, 12/12 hr light/dark</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1002" data-index="3" style="margin-left:35px;">1 tube set at 20˚C in light incubator, 2 set at room temp</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1003" data-index="4" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1004" data-index="5" style="margin-left:35px;">Tubes at room temp didn't grow, tube in light incubator had thin layer of alga growing on top of media. All tubes (including original agar tube) set in light incubator at ~21 W/m<sup>2</sup></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1005" data-index="6" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1006" data-index="7" style="margin-left:35px;">500ul taken from one of three tubes in the light incubator and put in a flask with BG-11</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1007" data-index="8" style="margin-left:70px;">Media lacks sulfur; is a test for hydrogen production capabilities</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1008" data-index="9" style="margin-left:35px;">Checked flask, full of growth! All good. Gas production should be good to go</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1009" data-index="10" style="margin-left:35px;">Flask with BG-11 prepared with water balloon and nozzle up top, left overnight to see if gas production occurred and balloon inflated</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1010" data-index="11" style="margin-left:35px;">Flask is moved to Stanford labs for further testing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1011" data-index="12" style="margin-left:35px;">When gas in flask is introduced to flame, a pop is heard characteristic of hydrogen gas</div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div>
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Difference between revisions of "Team:Stanford-Brown/SB16 Notebooks Gas"

Revision as of 02:31, 17 October 2016

Gas Production