Difference between revisions of "Team:Pittsburgh/Attributions"

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    <p>People who made our team and our project possible</p>
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        </div>
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<h2 style="padding-top:0;">References and Inspiration</h2>
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    <p>Our circuit is largely based off the following papers and groups:</p>
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    <p>the work with toehold switches, cell-free systems, and paper-based sensors detailed in <a href="http://www.cell.com/abstract/S0092-8674(14)01291-4" target="_blank">"Paper-Based Synthetic Gene Networks"</a> by Pardee et al., which we consistently refer to as "the Colli{{Pittsburgh}}
 
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     <p>Our circuit is largely based off the following papers and groups:</p>
 
     <p>Our circuit is largely based off the following papers and groups:</p>
 
     <p>the work with toehold switches, cell-free systems, and paper-based sensors detailed in <a href="http://www.cell.com/abstract/S0092-8674(14)01291-4" target="_blank">"Paper-Based Synthetic Gene Networks"</a> by Pardee et al., which we consistently refer to as "the Collins paper"; </p>
 
     <p>the work with toehold switches, cell-free systems, and paper-based sensors detailed in <a href="http://www.cell.com/abstract/S0092-8674(14)01291-4" target="_blank">"Paper-Based Synthetic Gene Networks"</a> by Pardee et al., which we consistently refer to as "the Collins paper"; </p>
 +
    <p>the <a href="https://2015.igem.org/Team:Pitt" target="_blank">2015 Pitt iGEM team</a> and their work with cell-free, paper-based systems and the Collins materials;</p>
 +
    <p>the thallium DNAzyme described by Huang, Vazin, and Liu in <a href="http://www.ncbi.nlm.nih.gov/pubmed/26393365" target="_blank">"Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium"</a>;</p>
 +
    <p>the amplification systems developed by Wang, Barahona, and Buck in <a href="http://nar.oxfordjournals.org/content/early/2014/07/15/nar.gku593.full">"Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks"</a>; and</p>
 +
    <p>toehold switch design described by the <a href="https://2015.igem.org/Team:Exeter" target="_blank"> 2015 Exeter iGEM team</a>.</p>
 +
 +
   
 +
<h2>Labs</h2>
 +
    <p>Huge thank-you's go to the following groups and people for making this year's iGEM team a success:</p>
 +
    <p><strong>Dave Gau</strong> from the Roy lab, who provided us with the various odds and ends we needed to grow cells;</p>
 +
    <p><strong>Steve</strong> from the Shroff lab, who provided us with reagents and materials for making buffers, a freezer, and friendliness every day;</p>
 +
    <p><strong>Neill Turner</strong> from the McGowan Institute for giving us access to a plate reader; and</p>
 +
    <p><strong>Nick Ankenbruck</strong> from the Deiters lab, who helped us run dPAGE gels.</p>
 +
   
 +
<h2>Advisors and Mentors</h2>
 +
    <p>Our <a href="https://2016.igem.org/Team:Pittsburgh/Team#Advisors" target="_blank">advisors and mentors</a> met with us every week to discuss ideas for the circuit, experimental results, experimental design, and presentations. Special thanks go to:</p>
 +
    <p><strong>Dr. Jason Lohmueller</strong>, who started iGEM at Pitt and provided valuable insights for experimental designs and interpretation of results;</p>
 +
    <p><strong>Dr. Sanjeev Shroff</strong>, who worked out all the logistics to make iGEM possible;</p>
 +
    <p><strong>Dr. Natasa Miskov-Romanov</strong>, who provided us with a lab space;</p>
 +
    <p><strong>Dr. Alex Deiters</strong>, who always knew we could do better; and, last but not least,</p>
 +
    <p><strong>Dr. Lisa Antoszewski</strong>. Without her dedication, patience, and organization as our laboratory mentor, we would never have passed the theoretical stages.</p>
 +
   
 +
<h2>Team</h2>
 +
   
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<h2>Sponsors</h2>
 +
    <p>Thank you to <strong>Breton Hornblower</strong> and <strong>Cory Tuckey</strong> from NEB for making PURExpress a viable option for our experiments.</p>
 +
   
 +
<h2>Images</h2>
 +
    <p>Our wiki contains images from the following sources:</p>
 +
    <p>Claire Chu, for the team logos;</p>
 +
    <p>Wikipedia, for "Oakland Viewed From Flagstaff Hill" by Ajblanck, used for the banner;</p>
 +
    <p>Chaoyang Wang, for the Mid-Atlantic Meet-Up;</p>
 +
    <p>Claire Chu, for Washington, D.C.;</p>
 +
    <p>Bestofpicture.com, for the notebook clipart used in the Notebook;</p>
 +
   
 +
   
 +
<h2>Project Timeline</h2>
 +
    <p>The team met for the first time at the beginning of April. For the next month-and-a-half, we brainstormed ideas for our project. By our first day in lab at the end of May, we knew we wanted to design a thallium sensor. Our circuit design solified over the next week or so as we started to familiarize ourselves with the lab space. By the next week, we were learning lab techniques and applying them to our project. See our <a href="2016.igem.org/Team:Pittsburgh/Notebook" target="_blank">lab notebook</a> for more details on what we worked on each week.</p>
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    </div>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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</div>
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<div class="clear"></div>
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<h5> Why is this page needed? </h5>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<h5> What should this page have?</h5>
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<ul>
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<li>General Support</li>
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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</ul>
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<h5>Inspiration</h5>
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<p>Take a look at what other teams have done:</p>
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<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
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<h5>Team training and Project start</h5>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<ul>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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</ul>
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</div>
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</div>
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ns paper"; </p>
 
     <p>the <a href="https://2015.igem.org/Team:Pitt" target="_blank">2015 Pitt iGEM team</a> and their work with cell-free, paper-based systems and the Collins materials;</p>
 
     <p>the <a href="https://2015.igem.org/Team:Pitt" target="_blank">2015 Pitt iGEM team</a> and their work with cell-free, paper-based systems and the Collins materials;</p>
 
     <p>the thallium DNAzyme described by Huang, Vazin, and Liu in <a href="http://www.ncbi.nlm.nih.gov/pubmed/26393365" target="_blank">"Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium"</a>;</p>
 
     <p>the thallium DNAzyme described by Huang, Vazin, and Liu in <a href="http://www.ncbi.nlm.nih.gov/pubmed/26393365" target="_blank">"Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium"</a>;</p>

Revision as of 17:32, 2 August 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

People who made our team and our project possible

References and Inspiration

Our circuit is largely based off the following papers and groups:

the work with toehold switches, cell-free systems, and paper-based sensors detailed in "Paper-Based Synthetic Gene Networks" by Pardee et al., which we consistently refer to as "the Colli{{Pittsburgh}}

People who made our team and our project possible

References and Inspiration

Our circuit is largely based off the following papers and groups:

the work with toehold switches, cell-free systems, and paper-based sensors detailed in "Paper-Based Synthetic Gene Networks" by Pardee et al., which we consistently refer to as "the Collins paper";

the 2015 Pitt iGEM team and their work with cell-free, paper-based systems and the Collins materials;

the thallium DNAzyme described by Huang, Vazin, and Liu in "Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium";

the amplification systems developed by Wang, Barahona, and Buck in "Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks"; and

toehold switch design described by the 2015 Exeter iGEM team.

Labs

Huge thank-you's go to the following groups and people for making this year's iGEM team a success:

Dave Gau from the Roy lab, who provided us with the various odds and ends we needed to grow cells;

Steve from the Shroff lab, who provided us with reagents and materials for making buffers, a freezer, and friendliness every day;

Neill Turner from the McGowan Institute for giving us access to a plate reader; and

Nick Ankenbruck from the Deiters lab, who helped us run dPAGE gels.

Advisors and Mentors

Our advisors and mentors met with us every week to discuss ideas for the circuit, experimental results, experimental design, and presentations. Special thanks go to:

Dr. Jason Lohmueller, who started iGEM at Pitt and provided valuable insights for experimental designs and interpretation of results;

Dr. Sanjeev Shroff, who worked out all the logistics to make iGEM possible;

Dr. Natasa Miskov-Romanov, who provided us with a lab space;

Dr. Alex Deiters, who always knew we could do better; and, last but not least,

Dr. Lisa Antoszewski. Without her dedication, patience, and organization as our laboratory mentor, we would never have passed the theoretical stages.

Team

Sponsors

Thank you to Breton Hornblower and Cory Tuckey from NEB for making PURExpress a viable option for our experiments.

Images

Our wiki contains images from the following sources:

Claire Chu, for the team logos;

Wikipedia, for "Oakland Viewed From Flagstaff Hill" by Ajblanck, used for the banner;

Chaoyang Wang, for the Mid-Atlantic Meet-Up;

Claire Chu, for Washington, D.C.;

Bestofpicture.com, for the notebook clipart used in the Notebook;

Project Timeline

The team met for the first time at the beginning of April. For the next month-and-a-half, we brainstormed ideas for our project. By our first day in lab at the end of May, we knew we wanted to design a thallium sensor. Our circuit design solified over the next week or so as we started to familiarize ourselves with the lab space. By the next week, we were learning lab techniques and applying them to our project. See our lab notebook for more details on what we worked on each week.

ns paper"; </p>

the <a href="https://2015.igem.org/Team:Pitt" target="_blank">2015 Pitt iGEM team</a> and their work with cell-free, paper-based systems and the Collins materials;

the thallium DNAzyme described by Huang, Vazin, and Liu in <a href="http://www.ncbi.nlm.nih.gov/pubmed/26393365" target="_blank">"Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium"</a>;

the amplification systems developed by Wang, Barahona, and Buck in <a href="http://nar.oxfordjournals.org/content/early/2014/07/15/nar.gku593.full">"Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks"</a>; and

toehold switch design described by the <a href="https://2015.igem.org/Team:Exeter" target="_blank"> 2015 Exeter iGEM team</a>.


Labs

Huge thank-you's go to the following groups and people for making this year's iGEM team a success:

Dave Gau from the Roy lab, who provided us with the various odds and ends we needed to grow cells;

Steve from the Shroff lab, who provided us with reagents and materials for making buffers, a freezer, and friendliness every day;

Neill Turner from the McGowan Institute for giving us access to a plate reader; and

Nick Ankenbruck from the Deiters lab, who helped us run dPAGE gels.

Advisors and Mentors

Our <a href="https://2016.igem.org/Team:Pittsburgh/Team#Advisors" target="_blank">advisors and mentors</a> met with us every week to discuss ideas for the circuit, experimental results, experimental design, and presentations. Special thanks go to:

Dr. Jason Lohmueller, who started iGEM at Pitt and provided valuable insights for experimental designs and interpretation of results;

Dr. Sanjeev Shroff, who worked out all the logistics to make iGEM possible;

Dr. Natasa Miskov-Romanov, who provided us with a lab space;

Dr. Alex Deiters, who always knew we could do better; and, last but not least,

Dr. Lisa Antoszewski. Without her dedication, patience, and organization as our laboratory mentor, we would never have passed the theoretical stages.

Team

Sponsors

Thank you to Breton Hornblower and Cory Tuckey from NEB for making PURExpress a viable option for our experiments.

Images

Our wiki contains images from the following sources:

Claire Chu, for the team logos;

Wikipedia, for "Oakland Viewed From Flagstaff Hill" by Ajblanck, used for the banner;

Chaoyang Wang, for the Mid-Atlantic Meet-Up;

Claire Chu, for Washington, D.C.;

Bestofpicture.com, for the notebook clipart used in the Notebook;


Project Timeline

The team met for the first time at the beginning of April. For the next month-and-a-half, we brainstormed ideas for our project. By our first day in lab at the end of May, we knew we wanted to design a thallium sensor. Our circuit design solified over the next week or so as we started to familiarize ourselves with the lab space. By the next week, we were learning lab techniques and applying them to our project. See our <a href="2016.igem.org/Team:Pittsburgh/Notebook" target="_blank">lab notebook</a> for more details on what we worked on each week.

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