Difference between revisions of "Team:NCKU Tainan/Collaborations"
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| − | <div class="head"> | + | <div class="head">TEAM / Collaborations</div> |
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| − | <div class="head2"> | + | <div class="head2">Team - Collaborations</div> |
| + | <div class="title-line" id="section1">Protocols & Matericals</div> | ||
| + | <div class="intro">Collaborate with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a>, we shared the protocol and sent the material for 1000ml of Glucose-free modified M9 medium for cell culturing and functional experiments.</div> | ||
| + | <h5>Protocol for modified M9:</h5> | ||
| + | <p>For preparing 10X M9 stock | ||
| + | </p><ul><li>Formulation</li> | ||
| + | <li>Disodium Phosphate Heptahydrate 128 g/L</li> | ||
| + | <li>Monopotassium Phosphate 30 g/L</li> | ||
| + | <li>Sodium Chloride 5 g/L</li> | ||
| + | <li>Ammonium Chloride 10 g/L</li> | ||
| + | <li>Final pH (2X solution): 6.6–7.0 at 25°C</li> | ||
| + | </ul><p></p> | ||
| + | <p>After dilution to 1X and autoclave, for every 20 ml, add | ||
| + | </p><ul><li>20% D-Glucose solution 400ul</li> | ||
| + | <li>1.0 M MgSO4 solution 40ul</li> | ||
| + | <li>1.0 M CaCl2 solution 2ul</li> | ||
| + | <li>20% Casamino acid 200ul</li> | ||
| + | </ul><p></p> | ||
| + | <p>Modofied M9 Sample sent to <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a></p> | ||
| + | <img src="/wiki/images/d/d8/T--NCKU_Tainan--collaborations1.jpg"> | ||
| + | <div class="title-line" id="section2">Drylab</div> | ||
| + | <h5>Collaboration with <a href="https://2016.igem.org/Team:TCU_Taiwan" target="_blank">TCU_Taiwan</a></h5> | ||
| + | <p>Both of us are doing the project about glucose detection, we discuss some issues about how E. coli react when glucose is added and share the information and difficulties.</p> | ||
| + | <p>Further, we also provide some mathematical support on deriving the formula of production rate when system reaches its equilibrium.</p> | ||
| + | <img src="/wiki/images/8/8f/T--NCKU_Tainan--collaborations2.jpg"> | ||
| + | </div> | ||
| + | <div class="col-md-3"> | ||
| + | <ul id="sidemenu" style="width:90%"> | ||
| + | <li><a href="#" onclick="return toEvent('section1');">Protocols & Materials</a></li> | ||
| + | <li><a href="#" onclick="return toEvent('section2');">Drylab</a></li> | ||
| + | </ul> | ||
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Revision as of 20:01, 9 October 2016
TEAM / Collaborations
Team - Collaborations
Protocols & Matericals
Collaborate with TCU_Taiwan, we shared the protocol and sent the material for 1000ml of Glucose-free modified M9 medium for cell culturing and functional experiments.
Protocol for modified M9:
For preparing 10X M9 stock
- Formulation
- Disodium Phosphate Heptahydrate 128 g/L
- Monopotassium Phosphate 30 g/L
- Sodium Chloride 5 g/L
- Ammonium Chloride 10 g/L
- Final pH (2X solution): 6.6–7.0 at 25°C
After dilution to 1X and autoclave, for every 20 ml, add
- 20% D-Glucose solution 400ul
- 1.0 M MgSO4 solution 40ul
- 1.0 M CaCl2 solution 2ul
- 20% Casamino acid 200ul
Modofied M9 Sample sent to TCU_Taiwan
Drylab
Collaboration with TCU_Taiwan
Both of us are doing the project about glucose detection, we discuss some issues about how E. coli react when glucose is added and share the information and difficulties.
Further, we also provide some mathematical support on deriving the formula of production rate when system reaches its equilibrium.
