Difference between revisions of "Team:NCKU Tainan/Notebook"

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     <style>@font-face {  font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Description">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li>          <li><a href="/Team:NCKU_Tainan/Software">Software</a></li>          <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li>          <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
 
     <style>@font-face {  font-family: 'NotoSansCJKtc-Regular';  src: url("/wiki/images/0/0b/T--NCKU_Tainan--NotoSansCJKtc-Regular.woff") format('woff');}</style><nav class="navbar navbar-default"><div class="container-fluid" style="width:72%">  <div class="navbar-header">      <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false">        <span class="sr-only">Toggle navigation</span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>        <span class="icon-bar"></span>      </button>      <a class="navbar-brand" href="/Team:NCKU_Tainan">        <h1>NCKU</h1><h4>Tainan</h4>      </a>  </div>  <div id="navbar" class="navbar-collapse collapse">    <ul class="nav navbar-nav">      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Project</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Project">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Description">Description</a></li>          <li><a href="/Team:NCKU_Tainan/Results">Results</a></li>          <li><a href="/Team:NCKU_Tainan/Model">Modeling</a></li>          <li><a href="/Team:NCKU_Tainan/Parts">Parts</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu2" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Device</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu2">          <li><a href="/Team:NCKU_Tainan/Hardware">Hardware</a></li>          <li><a href="/Team:NCKU_Tainan/Software">Software</a></li>          <li><a href="/Team:NCKU_Tainan/Demonstrate">Demonstrate</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu3" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Judging</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu3">          <li><a href="/Team:NCKU_Tainan/Medal">Medal</a></li>          <li><a href="/Team:NCKU_Tainan/Safety">Safety</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu4" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Team">Team</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu4">          <li><a href="/Team:NCKU_Tainan/Team">Team</a></li>          <li><a href="/Team:NCKU_Tainan/Attributions">Attributions</a></li>          <li><a href="/Team:NCKU_Tainan/Acknowledgement">Acknowledgement</a></li>          <li><a href="/Team:NCKU_Tainan/Collaborations">Collaborations</a></li>        </ul>        </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu5" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="/Team:NCKU_Tainan/Human_Practices">Human Practices</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu5">          <li><a href="/Team:NCKU_Tainan/Human_Practices">Overview</a></li>          <li><a href="/Team:NCKU_Tainan/Integrated_Practices">Integrated Practices</a></li>          <li><a href="/Team:NCKU_Tainan/Engagement">Engagement</a></li>        </ul>      </li>      <li>        <a class="dropdown-toggle" type="button" id="dropdownMenu6" data-toggle="dropdown" aria-haspopup="true" aria-expa="" nded="true" href="">Notebook</a><div class="nav-underline"></div>        <ul class="dropdown-menu" aria-labelledby="dropdownMenu6">          <li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>          <li><a href="/Team:NCKU_Tainan/Notebook_Protocols">Protocols</a></li>        </ul>      </li>    </ul>    <ul class="nav navbar-nav navbar-right">    </ul>  </div><!--/.nav-collapse --></div><!--/.container-fluid --></nav><div id="container-big"><div id="iGEMbar"></div><div id="line-left"></div><div id="line-left2"></div><div id="line-right"></div><div id="photo-left"></div></div><!--/.container-big --> <style>
 
#photo-left { background-image: url("/wiki/images/c/c1/T--NCKU_Tainan--sample2.jpg"); }
 
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<div class="container-fluid" style="margin-top:100px">
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<div class="head">NOTE / Construction</div>
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<div class="head">NOTEBOOK / Overview</div>
 
<div class="content row">
 
<div class="content row">
 
<div class="col-md-9">
 
<div class="col-md-9">
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<div class="head2">Notebook - Construction</div>
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<div class="head2">Notebook - Overview</div>
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<div class="title-line" id="sec1"></div>
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<div class="intro">We divide our record - notebook into four parts:</div>
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<div class="title-content">Extracted plasmid from the bacteria provided by XMU<br>(pSB1C3-BBa_B0034, pSB1C3-BBa_B0015, pSB1C3-BBa_K592009)</div>
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<ol>
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<h5>2016.03.30</h5>
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<li><a href="/Team:NCKU_Tainan/Notebook_Construction">Construction</a></li>
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<p>We got the bacteria with Biobrick from XMU.</p>
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<li><a href="/Team:NCKU_Tainan/Notebook_Functional_Test">Functional Test</a></li>
−
<h5>2016.04.01</h5>
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<li><a href="/Team:NCKU_Tainan/Notebook_Device_Design">Device Design</a></li>
−
<p>Pre-culture: 3 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate.</p>
+
<li><a href="#">Protocols</a></li>
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<h5>2016.04.02</h5>
+
</ol>
−
<p>Culture: 10 ml of LB (with Chloramphenicol) culture with 100 ml/μL pre-cultured bacteria. </p>
+
−
<p>And grow up at 37 degree for 10-12 hours.</p>
+
−
<h5>2016.04.03</h5>
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−
<p>Extracted the plasmid, and measured the concentration.</p>
+
−
<p>Store the plasmid at -20 degree.</p>
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−
 
+
−
<div class="title-line" id="sec2"></div>
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−
<div class="title-content">Got the glucose sensitive promoter (PpI, Pcar) by PCR</div>
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−
<h5>2016.04.16</h5>
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−
<p>PCR for PpI &amp; Pcar.<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: After gel purified, the concentration of the DNA was too low to do construction.</p>
+
−
<h5>2016.04.17</h5>
+
−
<p>PCR for PpI &amp; Pcar again (in big scale).</p>
+
−
            <p>Purified the PCR product and cloned to T-vector.</p>
+
−
<h5>2016.04.18</h5>
+
−
<p>Confirmed the white colony by PCR (using M13F &amp; M13R as the primer)<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: Some of the colonies were correct.</p>
+
−
            <p>Pre-culture: 3 ml of LB (with Ampicillin) was inoculated with one correct colony from the agar plate.</p>
+
−
<h5>2016.04.19</h5>
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−
<p>Extracted plasmid and confirmed plasmid size by gel electrophoresis.<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: The size of pMD19T-PpI and pMD19T-Pcar were correct.</p>
+
−
<h5>2016.04.20</h5>
+
−
<p>Culture: 10 ml of LB (with Ampicillin) cultured with 100 ml/μL pre-cultured bacteria.</p>
+
−
            <p>Grow up at 37 degree for 8 hours and stored the bacteria at -70 refrigerator.</p>
+
−
 
+
−
            <div class="title-line" id="sec3"></div>
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−
<div class="title-content">Constructed pSB1C3-B0034-K592009</div>
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−
<h5>2016.04.30</h5>
+
−
            <p>Digested pSB1C3-B0034 (<i>SpeI,PstI</i>) and pSB1C3-K592009 (<i>XbaI,PstI</i>)</p>
+
−
            <p>Ligated K592009 (insert) to pSB1C3-B0034 (vector) and transformed the product.</p>
+
−
            <h5>2016.05.01</h5>
+
−
            <p>Confirmed by colony PCR (using Prefix-F &amp; Suffix-R as the primer).</p>
+
−
            <p>Pre-culture: 3 ml of LB (with Chloramphenicol) was inoculated with one correct colony from the agar plate.</p>
+
−
            <h5>2016.05.02</h5>
+
−
            <p>Extracted plasmid and confirmed plasmid size by gel electrophoresis.<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: The size of pSB1C3-B0034-K592009 was correct.
+
−
</p>
+
−
            <h5>2016.05.03</h5>
+
−
            <p>Culture: 10 ml of LB (with Chloramphenicol) cultured with 100ml/μL pre-cultured bacteria.</p>
+
−
            <p>Grow up at 37 degree for 8 hours and stored the bacteria at -70 refrigerator.</p>
+
−
 
+
−
            <div class="title-line" id="sec3"></div>
+
−
<div class="title-content">Construct pMD19T-Pcar-B0034-K592009 and pMD19T-PpI-B0034-K592009</div>
+
−
<h5>2016.05.07</h5>
+
−
            <p>Digested pSB1C3-B0034-K592009 (<i>XbaI,PstI</i>), pMD19T-Pcar (<i>SpeI,PstI</i>) and pMD19T-PpI (<i>SpeI,PstI</i>)
+
−
            </p><p>Ligated B0034-K592009 (insert) to pMD19T-Pcar (vector) and pMD19T-Pcar (vector). </p>
+
−
            <p>Transformed the product.</p>
+
−
            <h5>2016.05.08</h5>
+
−
            <p>Confirmed by colony PCR (using M13F &amp; M13R as the primer).</p>
+
−
            <p>Pre-culture: 3 ml of LB (with Ampicillin) was inoculated with one correct colony from the agar plate.</p>
+
−
            <h5>2016.05.09</h5>
+
−
            <p>Extracted plasmid and confirm plasmid size by gel electrophoresis.<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: The size of pMD19T-Pcar-B0034-K592009 and pMD19T-PpI-B0034-K592009 were correct.</p>
+
−
            <h5>2016.05.10</h5>
+
−
            <p>Culture: 10 ml of LB (with Ampicillin) cultured with 100 ml/μL pre-cultured bacteria.</p>
+
−
            <p>Grow up at 37 degree for 8 hours and stored the bacteria at -70 refrigerator.</p>
+
−
 
+
−
            <div class="title-line" id="sec3"></div>
+
−
<div class="title-content">We transformed BioBricks from the 2016 igem distribution<br>
+
−
              (BBa_J23110, BBa_J23101,BBa_B0031,BBa_B0032,BBa_E1010)</div>
+
−
            <h5>2016.06.03</h5>
+
−
            <p>Transformed 3 ml/μL biobrick to 100 ml/μL DH5α competent cell by heat shock.<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: The transformation of BBa_J23110, BBa_B0032 and BBa_E1010 was successful.<br>
+
−
              <i class="glyphicon glyphicon-remove"></i>Result: The transformation of BBa_J23110, BBa_B0032 and BBa_E1010 was fail.</p>
+
−
            <h5>2016.06.04</h5>
+
−
            <p>Pre-culture: 4 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate.</p>
+
−
            <p>Extracted plasmid by Plasmid miniPREP Kit and confirmed plasmid size by gel electrophoresis.</p>
+
−
            <h5>2016.06.05</h5>
+
−
            <p>Culture: 10 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate. And grow up at 37 degree for 10-12 hours.</p>
+
−
            <h5>2016.06.06</h5>
+
−
            <p>Extracted the plasmid, and measure the concentration.</p>
+
−
            <p>Stored the plasmid at -20 degree.</p>
+
−
            <h5>2016.06.07</h5>
+
−
            <p>Transformed 5 ml/μL biobrick to 100 ml/μL DH5α competent cell by heat shock.<br>
+
−
              (BBa_J23110, BBa_B0032 and BBa_E1010)<br>
+
−
              <i class="glyphicon glyphicon-ok"></i>Result: The transformation of BBa_J23110, BBa_B0032 and BBa_E1010 were successful.</p>
+
−
            <h5>2016.06.08</h5>
+
−
            <p>Pre-culture: 4 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate.</p>
+
−
            <p>Extracted plasmid by Plasmid miniPREP Kit and confirmed plasmid size by gel electrophoresis.</p>
+
−
            <h5>2016.06.09</h5>
+
−
            <p>Culture: 10 ml of LB (with Chloramphenicol) was inoculated with one colony from LB agar plate.</p>
+
−
            <p>And grow up at 37 degree for 10-12 hours.</p>
+
−
            <h5>2016.06.10</h5>
+
−
            <p>Extracted the plasmid, and measure the concentration.</p>
+
−
            <p>Stored the plasmid at -20 degree.</p>
+
−
 
+
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
−
<ul id="sidemenu">
 
−
<li><a href="#" onclick="toEvent('section1');">Section 1</a></li>
 
−
<li><a href="#" onclick="toEvent('section2');">Section 2</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 20:19, 9 October 2016

Notebook - iGEM NCKU

NOTEBOOK / Overview
Notebook - Overview
We divide our record - notebook into four parts:
  1. Construction
  2. Functional Test
  3. Device Design
  4. Protocols