Difference between revisions of "Team:NAU-CHINA/Description"

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<p>The main metabolite in the degradation of insecticide pyrethroids in mammals, insects, fungi, and bacteria, is <strong>3-phenoxybenzoate (3-PBA)</strong>, a diaryl ether compound.For the wide use of pyrethroids and the stability of the diaryl ether compound itself, 3-PBA is typically detected as an important environmental contaminant. </p>
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<p>The project is aiming to design an engineered bacteria that can degrade 3-PBA thoroughly <b>(as shown in picture1)</b> and efficiently in about 3 days.</p>
  
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<p><b><i>Sphingobium wenxiniae</i> JZ-1 ͭ  </b>can utilize 3-PBA as the sole carbon and energy sources for growth，with the 3-phenoxybenzoate (3-PBA) 1,2-dioxygenase gene cluster (pbaA1A2B cluster) and the reductase component gene pbaC, which are responsible for catalyzing 3-phenoxybenzoate（3-HBA）to 3-hydroxybenzoate and catechol. Also genes(mhbDHIM), from Klebsiella pneumoniae M5a1 which is capable of utilizing 3-hydroxybenzoate, encode enzymes converting 3-hydroxybenzoate (3-HBA) to pyruvate and fumarate via gentisate.</p>
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<p><b>3-PBA</b>, the main metabolite in the degradation of insecticide pyrethroids (widely used for insect control in agriculture industry), is typically detected as a vital environmental contaminant and half-life of 3-phenoxybenzoate (3-PBA) in soil is 180 days (conventional pesticides is 30 days).</p>
  
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<p>Therefore, our project is to design a device with engineered bacterias which are capable of degrading 3-PBA more efficiently.</p>
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<p>Microbial biodegradation is valued for its environment-friendly method to eliminate pollution. It is noted that <i>sphingobium wenxiniae JZ-1</i> is able to degrade 3-PBA into 3-hydroxybenzoate(3-HBA) in 3 days and Klebsiella pneumoniae M5a1 can convert 3—HBA to pyruvate and fumarate via gentisate. </p>
  
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<p>Employing microbial biodegradation techniques to degrade 3-PBA is both feasible and worthy of research.</p>
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<img src="https://static.igem.org/mediawiki/2016/7/71/NAU_China_description_pic01.gif">
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<b>Picture 1</b> Process of 3-PBA Degradation
  
 
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Revision as of 05:22, 30 June 2016

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The project is aiming to design an engineered bacteria that can degrade 3-PBA thoroughly (as shown in picture1) and efficiently in about 3 days.

3-PBA, the main metabolite in the degradation of insecticide pyrethroids (widely used for insect control in agriculture industry), is typically detected as a vital environmental contaminant and half-life of 3-phenoxybenzoate (3-PBA) in soil is 180 days (conventional pesticides is 30 days).

Microbial biodegradation is valued for its environment-friendly method to eliminate pollution. It is noted that sphingobium wenxiniae JZ-1 is able to degrade 3-PBA into 3-hydroxybenzoate(3-HBA) in 3 days and Klebsiella pneumoniae M5a1 can convert 3—HBA to pyruvate and fumarate via gentisate.

Employing microbial biodegradation techniques to degrade 3-PBA is both feasible and worthy of research.



Picture 1 Process of 3-PBA Degradation