Difference between revisions of "Team:Pittsburgh/Parts"

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         <p>The parts Pitt iGEM developed over the summer are listed below. While not all the parts were submitted, take a look at our proposed use of each by following the provided links.</p></div>
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         <p>The Parts we used to build our circuit</p></div>
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    <div class="notebook column full_size">
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        <p>The Parts Pitt iGEM developed over the summer are listed below. Take a look by following the provided links.</p>
 
         <table width=90% align=center class="parts">
 
         <table width=90% align=center class="parts">
 
             <tr><br><br><br>
 
             <tr><br><br><br>
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                   <td><h3><b>PART NAME</b></h3></td>
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                   <td><h3><b>Part Name</b></h3></td>
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                   <td><h3><b>PART NUMBER</b></h3></td>
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                   <td><h3><b>Part Description</b></h3></td>
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                  <td><h3><b>Part Number</b></h3></td>
  
 
             </tr>
 
             </tr>
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             <tr>
 
             <tr>
 
                   <td>T3 Promoter</td>
 
                   <td>T3 Promoter</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084000"> BBa_K2084000 </a></td>
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                <td>Promoter for the T3 RNA Polymerase</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084000" target="_blank"> BBa_K2084000 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>T3 RNA Polymerase</td>
 
                   <td>T3 RNA Polymerase</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084001"> BBa_K2084001 </a></td>
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                <td>Encodes the T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084001" target="_blank"> BBa_K2084001 </a></td>
 
             </tr>
 
             </tr>
  
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             <tr>
 
             <tr>
 
                   <td>pT7 Toehold </td>
 
                   <td>pT7 Toehold </td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084002"> BBa_K2084002 </a></td>
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                <td>Regulatory ribosome binding site with T7 RNAP promoter</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084002" target="_blank"> BBa_K2084002 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT7 Regulated T3 RNA Polymerase</td>
 
                   <td>pT7 Regulated T3 RNA Polymerase</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084003"> BBa_K2084003 </a></td>
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                <td>Composite made up of our pT7-Toehold, T3 RNAP, and a terminator. To be used to control and regulate sequences with a T3 RNAP promoter.</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084003" target="_blank"> BBa_K2084003 </a></td>
 
             </tr>
 
             </tr>
  
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             <tr>
 
             <tr>
 
                   <td>pT3 with RBS</td>
 
                   <td>pT3 with RBS</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084004"> BBa_K2084004 </a></td>
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                <td>T3 promoter with an RBS site added</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084004" target="_blank"> BBa_K2084004 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT7 Toehold Regulated LacZ</td>
 
                   <td>pT7 Toehold Regulated LacZ</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084005"> BBa_K2084005 </a></td>
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                <td>BBa_K2084002 with a mutagenized <i>lacZ</i></td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084005" target="_blank"> BBa_K2084005 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT3 Controlled GFP</td>
 
                   <td>pT3 Controlled GFP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084006"> BBa_K2084006 </a></td>
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                <td>T3 RNAP promoter site to translate GFP</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084006" target="_blank"> BBa_K2084006 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT7 Controlled T3 RNAP</td>
 
                   <td>pT7 Controlled T3 RNAP</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084007"> BBa_K2084007 </a></td>
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                <td>Translated by T7 RNAP to create T3 RNAP</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084007" target="_blank"> BBa_K2084007 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>T3 RNA Polymerase Amplifier</td>
 
                   <td>T3 RNA Polymerase Amplifier</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084008"> BBa_K2084008 </a></td>
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                <td>T3 promoter and gene</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084008" target="_blank"> BBa_K2084008 </a></td>
 
             </tr>
 
             </tr>
  
 
             <tr>
 
             <tr>
 
                   <td>pT3 Controlled LacZ</td>
 
                   <td>pT3 Controlled LacZ</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K2084009"> BBa_K2084009 </a></td>
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                <td>LacZ reporter regulated by T3 RNAP</td>
 +
                   <td><a href="http://parts.igem.org/Part:BBa_K2084009" target="_blank"> BBa_K2084009 </a></td>
 
             </tr>
 
             </tr>
  
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           <p> Pitt iGEM would like to thank the Collins Laboratory at MIT for the pT7 toehold structures that they kindly sent us. We would also like to thank the Voigt Laboratory at MIT for the pT3 controlled GFP construct.</p>-->
 
           <p> Pitt iGEM would like to thank the Collins Laboratory at MIT for the pT7 toehold structures that they kindly sent us. We would also like to thank the Voigt Laboratory at MIT for the pT3 controlled GFP construct.</p>-->
 
     </div>
 
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Revision as of 17:44, 19 October 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

The Parts we used to build our circuit

The Parts Pitt iGEM developed over the summer are listed below. Take a look by following the provided links.




Part Name

Part Description

Part Number
T3 Promoter Promoter for the T3 RNA Polymerase BBa_K2084000
T3 RNA Polymerase Encodes the T3 RNAP BBa_K2084001
pT7 Toehold Regulatory ribosome binding site with T7 RNAP promoter BBa_K2084002
pT7 Regulated T3 RNA Polymerase Composite made up of our pT7-Toehold, T3 RNAP, and a terminator. To be used to control and regulate sequences with a T3 RNAP promoter. BBa_K2084003
pT3 with RBS T3 promoter with an RBS site added BBa_K2084004
pT7 Toehold Regulated LacZ BBa_K2084002 with a mutagenized lacZ BBa_K2084005
pT3 Controlled GFP T3 RNAP promoter site to translate GFP BBa_K2084006
pT7 Controlled T3 RNAP Translated by T7 RNAP to create T3 RNAP BBa_K2084007
T3 RNA Polymerase Amplifier T3 promoter and gene BBa_K2084008
pT3 Controlled LacZ LacZ reporter regulated by T3 RNAP BBa_K2084009