Difference between revisions of "Team:Pittsburgh/Team"

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<p>Our weekly progress. For a list of our protocols, visit the <a href="https://2016.igem.org/Team:Pittsburgh/Protocols" target="_blank"> Protocols</a> page</p>
 
  
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<p>Meet the members of the 2016 Pittsburgh team!</p>
 
</div>
 
</div>
  
 
<div class="table column full_size">
 
<div class="table column full_size">
−
<h2 style="color:rgb(0,0,128)">Contents</h2>
+
<h2 style="color=rgb(0,0,128)">Contents</h2>
 
<ul class="table">
 
<ul class="table">
−
<li><a href="#Week1" class="table">Week 1: May 23 - May 27</a></li>
+
<li><a href="#Students" class="table">Students</a></li>
−
<li><a href="#Week2" class="table">Week 2: May 31 - June 3</a></li>
+
    <ul>
−
<li><a href="#Week3" class="table">Week 3: June 6 - June 12</a></li>
+
        <li><a href="#Claire" class="table">Claire Chu</a></li>
−
<li><a href="#Week4" class="table">Week 4: June 13 - June 17</a></li>
+
        <li><a href="#Maya" class="table">Maya Lemmon-Kishi</a></li>
−
<li><a href="#Week5" class="table">Week 5: June 20 - June 26</a></li>
+
        <li><a href="#Aife" class="table">Aife Ni Chochlain</a></li>
−
<li><a href="#Week6" class="table">Week 6: June 27 - July 3</a></li>
+
        <li><a href="#Praneeth" class="table">Praneeth Pedadda</a></li>
−
<li><a href="#Week7" class="table">Week 7: July 5 - July 8</a></li>
+
        <li><a href="#Maddie" class="table">Maddie Perdoncin</a></li>
−
<li><a href="#Week8" class="table">Week 8: July 11 - July 17</a></li>
+
    </ul>
−
<li><a href="#Week9" class="table">Week 9: July 18 - July 22</a></li>
+
<li><a href="#Advisors" class="table">Advisors and Mentors</a></li>
 +
   
 +
    <div class="imgWrap">
 +
    <a href="#Alex" class="table"><img src="https://static.igem.org/mediawiki/2016/f/f0/T--Pittsburgh--TeamAlex.jpg" style="width:125px;padding:5px;"></a><p class="imgDescription">Dr. Alex Deiters</p></div>
 +
   
 +
    <div class="imgWrap">
 +
        <a href="#Jason" class="table">Dr. Jason Lohmueller</a></div>
 +
    <div class="imgWrap">
 +
       
 +
    <a href="#Lisa" class="table"><img src="https://static.igem.org/mediawiki/2016/7/71/T--Pittsburgh--TeamLisa.jpg" style="width:125px;padding:5px;"></a><p class="imgDescription">Dr. Lisa Antoszewski</p></div>
 +
   
 +
    <div class="imgWrap">
 +
    <a href="#Natasa" class="table">Dr. Natasa Miskov-Zivanov</a></div>
 +
       
 +
    <div class="imgWrap"><a href="#Cheryl" class="table"><img src="https://static.igem.org/mediawiki/2016/f/fa/TeamPittsburghBioTelmer.jpg" style="width:125px; padding:5px;"></a><p class="imgDescription">Dr. Cheryl Telmer</p></div>
 +
   
 +
    <div class="imgWrap"><a href="#Sam" class="table"><img src="https://static.igem.org/mediawiki/2016/6/61/T--Pittsburgh--TeamSam.jpg" style="width:125px; padding:5px;"></a><p class="imgDescription">Dr. Samuel Dickerson</p></div>
 +
    <br>
 +
<li><a href="#pgh" class="table">The 'Burgh</a></li>
 +
   
 
</ul>
 
</ul>
 
</div>
 
</div>
  
 
<div class="notebook column full_size">
 
<div class="notebook column full_size">
−
<h1 class="nav"><a name="Week1" class="nav">Week 1: May 23 - May 27</a></h1>
+
<h1 class="nav"><a name="Students" class="nav">Students</a></h1>
−
<h2>Wet Lab</h2>
+
<tr>
−
<ul>
+
<h2 class="nav"><a name="Claire" class="nav">Claire Chu</a></h2>
−
<li>Training begins</li>
+
    <td>>image</td>
−
<li>Grow Top 10 <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#competent" target="_blank">competent cells</a>.</li>
+
    <td><p>BIO</p><br>
−
</ul>
+
    <a href="#Top">Back to Top</a></td>
−
<h2>Dry Lab</h2>
+
<h2 class="nav"><a name="Maya" class="nav">Maya Lemmon-Kishi</a></h2>
−
<ul>
+
    <p>BIO</p>
−
<li>Brainstorm genetic circuits for a thallium sensor</li>
+
    <a href="#Top">Back to Top</a>
−
<li>Lab safety training</li>
+
<h2 class="nav"><a name="Aife" class="nav">Aife Ni Chochlain</a></h2>
−
</ul>
+
    <p>BIO</p>
−
<a href=https://static.igem.org/mediawiki/2016/b/bc/TeamPittsburghNotebookWeek1.pdf target="_blank">Week 1 Notebook</a><br>
+
    <a href="#Top">Back to Top</a>
−
<a href="#Top">Back to Top</a>
+
<h2 class="nav"><a name="Praneeth" class="nav">Praneeth Peddada</a></h2>
 +
    <p>BIO</p>
 +
    <a href="#Top">Back to Top</a>
 +
<h2 class="nav"><a name="Maddie" class="nav">Maddie Perdoncin</a></h2>
 +
    <p>BIO</p>
 +
    <a href="#Top">Back to Top</a>
 +
</tr>
 +
</div>
  
−
<h1 class="nav"><a name="Week2" class="nav">Week 2: May 31 - June 3</a></h1>
+
<div class="notebook column full_size" >
−
<h2>Wet Lab</h2>
+
<h1 class="nav"><a name="Advisors" class="nav">Advisors and Mentors</a></h1>
−
<ul>
+
<table style="width:100%;">
−
     <li>Test <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#competent" target="_blank">efficiency</a> of competent cells</li>
+
<tr>  
−
</ul>
+
     <td style="vertical-align: top; width: 150px;"> <img class="bio" align="top" style="max-width: 150px; height: auto;" src="https://static.igem.org/mediawiki/2016/b/b9/TeamPittsburghBioDeiters.jpg"></td>
−
<h3>Cell-Free Extract</h3>
+
     <td style="vertical-align: top; padding-left:15px;"> <h2 class="nav"><a name="Alex" class="nav">Dr. Alex Deiters</a></h2>
−
     <ul><li>Test <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#cellfree" target="_blank">cell-free extract reaction</a> with T7-GFP plasmid</li></ul>
+
 
−
<h2>Dry Lab</h2>
+
    <p>Alex is a member of the University of Pittsburgh Cancer Institute, the Molecular Biophysics and Structural Biology Program at the University of Pittsburgh, the Medical Scientist Training Program at the University of Pittsburgh, and the Center for Nucleic Acids Science & Technology at Carnegie Mellon University. He has published over one hundred peer-reviewed papers, written five book chapters and 11 review articles, has presented over one hundred research seminars, and has consulted for several pharmaceutical companies.</p>
−
<ul>
+
−
<li>Contact museums and summer programs for outreach opportunities</li>
+
−
<li>Lab safety training</li>
+
−
</ul>
+
−
<a href=https://static.igem.org/mediawiki/2016/a/a8/TeamPittsburghNotebookWeek2.pdf target="_blank">Week 2 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
  
−
<h1 class="nav"><a name="Week3" class="nav">Week 3: June 6 - June 12</a></h1>
+
    <p>For his  research accomplishments, Alex received a Basil O'Connor Starter Scholar Award from the March of Dimes Foundation, a Sigma Xi Research Faculty Award, a Cottrell Scholar Award, a Beckman Young Investigator Award, a National Science Foundation CAREER Award, a Teva USA Scholars Grant from the American Chemical Society, a Thieme Chemistry Journal Award, an American Cancer Society Research Scholar Grant, Bill & Melinda Gates Foundation Grand Challenges Explorations Grant, an NCSU Alumni Association Outstanding Research Award, and a Charles E. Kaufman Foundation New Initiative Research Award.</p>
−
<h2>Wet Lab</h2>
+
    <a href="http://www.pitt.edu/~deiters/pi.html" target="_blank">Dr. Alex Deiters</a><br>
−
<h3>Reporter</h3>
+
     <a href="http://www.pitt.edu/~deiters/" target="_blank">Deiters Lab</a><br>
−
<ul>
+
     <a href="#Top">Back to Top</a></td>
−
     <li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#transformations" target="_blank">Transform</a> T7 promoter, amilCP, and terminator</li>
+
    </tr>
−
     <li>Begin assembly by <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#ligation" target="_blank">ligating</a> linearized T7 promoter and amilCP</li>
+
<tr>   
−
</ul>
+
        <td style="vertical-align: top; width: 150px;">image</td>
−
<h2>Dry Lab</h2>
+
        <td style="vertical-align: top; padding-left:15px;"><h2 class="nav"><a name="Jason" class="nav">Dr. Jason Lohmueller</a></h2><p>bio and info</p><br>
−
<ul>
+
    <a href="#Top">Back to Top</a></td>
−
<li>Contact museums and summer programs for outreach opportunities</li>
+
    </tr>
−
</ul>
+
<tr>
−
<a href=https://static.igem.org/mediawiki/2016/b/b3/TeamPittsburghNotebookWeek3.pdf target="_blank">Week 3 Notebook</a><br>
+
    <td style="vertical-align: top; width: 150px;">image</td>
−
<a href="#Top">Back to Top</a>
+
    <td style="vertical-align: top; padding-left:15px;"><h2 class="nav"><a name="Natasa" class="nav">Dr. Natasa Miskov-Zivanov</a></h2>
 +
    <p>bio and info</p>
 +
    <a href="#Top">Back to Top</a>
 +
    </td></tr>
  
−
<h1 class="nav"><a name="Week4" class="nav">Week 4: June 13 - June 17</a></h1>
+
<tr><td style="vertical-align: top; width: 150px;"><img class="bio" align="top" style="max-width: 150px; height: auto;" src="https://static.igem.org/mediawiki/2016/f/fa/TeamPittsburghBioTelmer.jpg"></td><td style="vertical-align: top; padding-left:15px;"><h2 class="nav"><a name="Cheryl" class="nav">Dr. Cheryl Telmer</a></h2>
−
<h2>Wet Lab</h2>
+
    <p>The goal of our research is to build biosensors for live-cell imaging. Methods that use light to visualize protein localization and interactions can be used to investigate the dynamics of cell structure and function. Another part of my research effort is directed towards a project named Big Mechanisms in Cancer where the goal of the program is to develop technologies for automated reading of scientific literature and development of causal, explanatory models of signaling pathways in cancer.</p>
−
<h3>Reporter</h3>
+
    <a href="http://bruchez-lab.mbic.cmu.edu/" target="_blank">Bruchez Lab</a> <br>
−
<ul>
+
    <a href="#Top">Back to Top</a></td>
−
<li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#transformations" target="_blank">Transform</a> T7-GFP plasmid, lacZ alpha fragment, and eGFP</li>
+
    </tr>
−
<li>Send promising T7 promoter -- amilCP ligations to be <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#sequencing" target="_blank">sequenced</a></li>
+
−
<li>Perform <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#digest" target="_blank">double digest</a> of T7 promoter and terminator from last week</li>
+
−
<li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#ligation" target="_blank">Ligate</a> double-digested T7 promoter to new reporters (lacZ and eGFP)</li>
+
−
</ul>
+
−
<h2>Dry Lab</h2>
+
−
<ul>
+
−
<li>TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set</li>
+
−
</ul>
+
−
<a href=https://static.igem.org/mediawiki/2016/7/75/TeamPittsburghNotebookWeek4.pdf target="_blank">Week 4 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
  
−
<h1 class="nav"><a name="Week5" class="nav">Week 5: June 20 - June 26</a></h1>
+
<tr><td style="vertical-align: top; width: 150px;"><img class="bio" align="top" style="max-width: 150px; height: auto;" src="https://static.igem.org/mediawiki/2016/d/dc/TeamPittsburghBioAntoszewski.jpg"></td>
−
<h2>Wet Lab</h2>
+
    <td style="vertical-align: top; padding-left:15px;"><h2 class="nav"><a name="Lisa" class="nav">Dr. Lisa Antoszewski</a></h2>
−
<h3>Reporter</h3>
+
         <p>Dr. Lisa Antoszewski is an adjunct faculty member in the Department of Developmental Biology at the University of Pittsburgh.  Using zebrafish as a model organism, her primary research interest is in kidney development.  More specifically, she is interested in identifying novel genes responsible for renal progenitor cell maintenance.  She is serving as a laboratory mentor for the 2016 iGEM team.</p>
−
<ul>
+
     <a href="#Top">Back to Top</a></td></tr>
−
    <li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#ligation" target="_blank">Ligate</a> T7 promoter -- amilCP construct to terminator</li>
+
 
−
<li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#extraction" target="_blank">Extract</a> successful ligations of T7 promoter to eGFP</li>
+
<tr><td style="vertical-align: top; width: 150px;"><img class="bio" align="top" style="max-width: 150px; height: auto;" src="https://static.igem.org/mediawiki/2016/b/b6/TeamPittsburghBioDickerson.jpg"></td><td style="vertical-align: top; padding-left:15px;"><h2 class="nav"><a name="Sam" class="nav">Dr. Samuel Dickerson</a></h2>
−
    <li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#ligation" target="_blank">Ligate</a> T7 promoter -- eGFP construct to terminator</li>
+
−
</ul>
+
−
<h3>Cell-Free Extract</h3>
+
−
    <ul>
+
−
         <li>Reaction volume can be reduced to 5 μL with 5 ng/μL of DNA</li>
+
−
     </ul>
+
−
<h3>Toehold Switch</h3>
+
−
    <ul>
+
−
        <li>Collins triggers activate the switches (both in plasmid form) to express LacZ</li>
+
−
    </ul>
+
−
<h2>Dry Lab</h2>
+
−
<ul>
+
−
<li>Reach out to teams to collaborate based on last year's projects</li>
+
−
</ul>
+
−
<a href="https://static.igem.org/mediawiki/2016/7/7c/TeamPittsburghNotebookWeek5.pdf" target="_blank">Week 5 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
 
      
 
      
−
<h1 class="nav"><a name="Week6" class="nav">Week 6: June 27 - July 3</a></h1>
+
     <p>Dr. Samuel Dickerson is an assistant professor in the Department of Electrical and Computer Engineering at the University of Pittsburgh. His research interests are in the design and modeling of nanoscale circuits and systems. Currently, he is investigating the use of electrokinetic techniques to sort, separate and analyze cells.</p>
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<h2>Wet Lab</h2>
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     <a href="#Top">Back to Top</a></td></tr>
−
<h3>Reporter</h3>
+
−
<ul>
+
−
     <li>Identify successful ligations to terminator for amilCP and eGFP consturcts using a <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#agarosegel" target="_blank">gel</a></li>
+
−
    <li>Send correct plasmids for <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#sequencing" target="_blank">sequencing</a> for confirmation</li>
+
−
    <li>Test plasmids in <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#cellfree" target="_blank">cell-free extract</a></li>
+
−
    <li>amilCP does not produce color in cell-free reaction</li>
+
−
    <li>eGFP produces fluorescence comparable to that from the Collins T7-GFP plasmid in cell-free reaction</li>
+
−
    <li>Linearized plasmids containing only the promoter and insert (no terminator) do not express protein</li>
+
−
</ul>
+
−
<h3>Cell-Free Extract</h3>
+
−
    <ul>
+
−
        <li>Reaction volume can be reduced to 1 μL with 5 ng/μL of DNA</li>
+
−
    </ul>
+
−
<h3>Toehold Switch</h3>
+
−
    <ul>
+
−
        <li>Collins plasmids express LacZ with 25 ng of switch</li>
+
−
        <li>DNA oligos trigger Collins switches</li>
+
−
     </ul>
+
−
<h2>Dry Lab</h2>
+
−
<ul>
+
−
<li>Work on outreach presentation for tissue engineering camp</li>
+
−
</ul>
+
−
<a href="https://static.igem.org/mediawiki/2016/5/59/T--Pittsburgh--NotebookWeek6.pdf" target="_blank">Week 6 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
  
 +
</table>
 +
 +
<h1 class="nav"><a name="pgh" class="nav">Welcome to Pittsburgh!</a></h1>
 
      
 
      
−
<h1 class="nav"><a name="Week7" class="nav">Week 7: July 5 - July 8</a></h1>
+
     <h4>Quick Facts</h4>
−
<h2>Wet Lab</h2>
+
     <p>Nickname: The Steel City</p>
−
<h3>Reporter</h3>
+
     <p>Dialect: Pittsburghese</p>
−
<ul>
+
     <p>Sports: Stillers, Pens, and Bucs (that’s Steelers, Penguins, and Pirates to you)</p>
−
     <li>Sequenced amilCP construct does not contain amilCP</li>
+
     <p>Bridges: 446. Our lab is right next to the Hot Metal Bridge</p>
−
     <li>Unsuccessfully linearize and amplify eGFP construct using <a href="https://2016.igem.org/Team:Pittsburgh/Protocols#pcr" target="_blank">PCR</a></li>
+
     <p>Neighborhoods: 90</p>
−
</ul>
+
     <p>Population: 305,704 yinzers </p>
−
<h3>Cell-Free Extract</h3>
+
     <p>Food: Primanti’s sandwiches, pierogies, Heinz ketchup</p>
−
     <ul>
+
−
        <li>384-well plate requires at least 10 μL of reaction</li>
+
−
     </ul>
+
−
<h3>DNAzyme</h3>
+
−
    <ul>
+
−
        <li><a href="https://2016.igem.org/Team:Pittsburgh/Protocols#annealing" target="_blank">Anneal</a> PO strand with catalytic strand, both with and without erbium</li>
+
−
        <li>Test success of annealing reaction in cell-free extract and with acrylamide gels</li>
+
−
     </ul>
+
−
<h2>Dry Lab</h2>
+
−
<ul>
+
−
<li>Practice outreach presentation for tissue engineering camp</li>
+
−
<li>Develop DNAzymes for other heavy metals</li>
+
−
</ul>
+
−
<a href="https://static.igem.org/mediawiki/2016/d/d7/T--Pittsburgh--NotebookWeek7.pdf" target="_blank">Week 7 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
−
   
+
−
<h1 class="nav"><a name="Week8" class="nav">Week 8: July 11 - July 17</a></h1>
+
−
<h2>Wet Lab</h2>
+
−
<h3>Reporter</h3>
+
−
<ul>
+
−
    <li>Restart amilCP cloning process</li>
+
−
</ul>
+
−
<h3>Cell-Free Extract</h3>
+
−
    <ul>
+
−
        <li>Linear eGFP construct does not produce a stronger signal than its plasmid form</li>
+
−
    </ul>
+
−
<h3>DNAzyme</h3>
+
−
    <ul>
+
−
        <li>DNAzyme duplex does not trigger toehold switch</li>
+
−
        <li>Erbium cleaves the P substrate strand</li>
+
−
     </ul>
+
−
<h2>Dry Lab</h2>
+
−
<ul>
+
−
<li>First presentation at Camp BioE</li>
+
−
<li>Prepare for UMD Mid-Atlantic Meet-Up</li>
+
−
<li>Contact PLSG and NEB for sponsorship</li>
+
−
</ul>
+
−
<a href="https://static.igem.org/mediawiki/2016/c/cb/T--Pittsburgh--NotebookWeek8.pdf" target="_blank">Week 8 Notebook</a><br>
+
−
<a href="#Top">Back to Top</a>
+
−
      
+
−
<h1 class="nav"><a name="Week9" class="nav">Week 9: July 18 - July 22</a></h1>
+
−
<h2>Wet Lab</h2>
+
−
<h3>Reporter</h3>
+
−
<ul>
+
−
    <li>Continue amilCP cloning process</li>
+
−
    <li>Clone RBS-T3 RNA polymerase to add into other constructs</li>
+
−
</ul>
+
−
<h3>Cell-Free Extract</h3>
+
−
    <ul>
+
−
        <li>Reactions can be diluted by one-half and still produce visible results in two hours</li>
+
−
    </ul>
+
−
<h3>DNAzyme</h3>
+
−
    <ul>
+
−
        <li>dPAGE assay of P substrate cleavage suggests that the DNAzyme works, but results are not definitive</li>
+
−
        <li>Six-hour time course of cleavage does not yield much additional information</li>
+
−
        <li>Reaction temperature (room temperature versus 37°C) does not produce observable effect on cleavage rates</li>
+
−
     </ul>
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<h2>Dry Lab</h2>
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<ul>
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<li>Presentation at Camp BioE</li>
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<li>UMD Mid-Atlantic Meet-Up</li>
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<li>Continue fundraising</li>
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<li>Discuss systems to model</li>
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</ul>
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<a href="https://static.igem.org/mediawiki/2016/7/75/T--Pittsburgh--NotebookWeek9.pdf" target="_blank">Week 9 Notebook</a><br>
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<a href="#Top">Back to Top</a>
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    <h4>Lab Adventures</h4>
 +
    <h5>Penguins Stanley Cup Parade</h5>
 +
    <p>The Penguins are the 2016 Stanley Cup Champions! The city celebrated with a parade, and we walked “dahntahn” along to avoid traffic and take part in the festivities. The real attraction was a real live penguin, courtesy of the PPG Aquarium. The cup was nice, too.</p>
 +
 +
    <h4>Other Pittsburgh Happenings</h4>
 +
    <h5>OpenStreets PGH</h5>
 +
    <p>BikePGH encouraged the city to get out and move this summer by closing off three miles of streets for bikers, walkers, joggers, and skaters. </p>
 +
 +
    <h5>Picklesburgh</h5> 
 +
    <p>Heinz sells more than ketchup--it sells pickles, too! Pickles were the star of the show on the Rachel Carson Bridge for the weekend of Picklesburgh. Some special finds included pickle egg rolls, pickle funnel cakes, and pickle merchandise.</p>
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</div>
 
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<h5>What should this page have?</h5>
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<h5>What should this page contain?</h5>
 
<ul>
 
<ul>
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<li>Chronological notes of what your team is doing.</li>
+
<li> Include pictures of your teammates, don’t forget instructors and advisors! </li>
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<li> Brief descriptions of daily important events.</li>
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<li>You can add a small biography or a few words from each team member, to tell us what you like, and what motivated you to participate in iGEM.</li>
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<li>Pictures of your progress. </li>
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<li>Take team pictures! Show us your school, your lab and little bit of your city.</li>
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<li>Mention who participated in what task.</li>
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<li>Remember that image galleries can help you showcase many pictures while saving space.</li>
 
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<h5>Inspiration</h5>
 
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<p>You can see what others teams have done to organize their notes:</p>
 
  
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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Revision as of 22:15, 28 July 2016

Thank you to our sponsors!

Office of the Provost Department of Bioengineering Department of Biological Sciences Department of Chemistry Swanson School of Engineering
IDT NEB Experiment

Meet the members of the 2016 Pittsburgh team!

Contents

Students

Claire Chu

>image

BIO


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Maya Lemmon-Kishi

BIO

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Aife Ni Chochlain

BIO

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Praneeth Peddada

BIO

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Maddie Perdoncin

BIO

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Advisors and Mentors

Dr. Alex Deiters

Alex is a member of the University of Pittsburgh Cancer Institute, the Molecular Biophysics and Structural Biology Program at the University of Pittsburgh, the Medical Scientist Training Program at the University of Pittsburgh, and the Center for Nucleic Acids Science & Technology at Carnegie Mellon University. He has published over one hundred peer-reviewed papers, written five book chapters and 11 review articles, has presented over one hundred research seminars, and has consulted for several pharmaceutical companies.

For his research accomplishments, Alex received a Basil O'Connor Starter Scholar Award from the March of Dimes Foundation, a Sigma Xi Research Faculty Award, a Cottrell Scholar Award, a Beckman Young Investigator Award, a National Science Foundation CAREER Award, a Teva USA Scholars Grant from the American Chemical Society, a Thieme Chemistry Journal Award, an American Cancer Society Research Scholar Grant, Bill & Melinda Gates Foundation Grand Challenges Explorations Grant, an NCSU Alumni Association Outstanding Research Award, and a Charles E. Kaufman Foundation New Initiative Research Award.

Dr. Alex Deiters
Deiters Lab
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image

Dr. Jason Lohmueller

bio and info


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image

Dr. Natasa Miskov-Zivanov

bio and info

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Dr. Cheryl Telmer

The goal of our research is to build biosensors for live-cell imaging. Methods that use light to visualize protein localization and interactions can be used to investigate the dynamics of cell structure and function. Another part of my research effort is directed towards a project named Big Mechanisms in Cancer where the goal of the program is to develop technologies for automated reading of scientific literature and development of causal, explanatory models of signaling pathways in cancer.

Bruchez Lab
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Dr. Lisa Antoszewski

Dr. Lisa Antoszewski is an adjunct faculty member in the Department of Developmental Biology at the University of Pittsburgh. Using zebrafish as a model organism, her primary research interest is in kidney development. More specifically, she is interested in identifying novel genes responsible for renal progenitor cell maintenance. She is serving as a laboratory mentor for the 2016 iGEM team.

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Dr. Samuel Dickerson

Dr. Samuel Dickerson is an assistant professor in the Department of Electrical and Computer Engineering at the University of Pittsburgh. His research interests are in the design and modeling of nanoscale circuits and systems. Currently, he is investigating the use of electrokinetic techniques to sort, separate and analyze cells.

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Welcome to Pittsburgh!

Quick Facts

Nickname: The Steel City

Dialect: Pittsburghese

Sports: Stillers, Pens, and Bucs (that’s Steelers, Penguins, and Pirates to you)

Bridges: 446. Our lab is right next to the Hot Metal Bridge

Neighborhoods: 90

Population: 305,704 yinzers

Food: Primanti’s sandwiches, pierogies, Heinz ketchup

Lab Adventures

Penguins Stanley Cup Parade

The Penguins are the 2016 Stanley Cup Champions! The city celebrated with a parade, and we walked “dahntahn” along to avoid traffic and take part in the festivities. The real attraction was a real live penguin, courtesy of the PPG Aquarium. The cup was nice, too.

Other Pittsburgh Happenings

OpenStreets PGH

BikePGH encouraged the city to get out and move this summer by closing off three miles of streets for bikers, walkers, joggers, and skaters.

Picklesburgh

Heinz sells more than ketchup--it sells pickles, too! Pickles were the star of the show on the Rachel Carson Bridge for the weekend of Picklesburgh. Some special finds included pickle egg rolls, pickle funnel cakes, and pickle merchandise.