Team:NAU-CHINA/Description
The main metabolite in the degradation of insecticide pyrethroids in mammals, insects, fungi, and bacteria, is 3-phenoxybenzoate (3-PBA), a diaryl ether compound.For the wide use of pyrethroids and the stability of the diaryl ether compound itself, 3-PBA is typically detected as an important environmental contaminant.
Sphingobium wenxiniae JZ-1 ͭ can utilize 3-PBA as the sole carbon and energy sources for growth，with the 3-phenoxybenzoate (3-PBA) 1,2-dioxygenase gene cluster (pbaA1A2B cluster) and the reductase component gene pbaC, which are responsible for catalyzing 3-phenoxybenzoate（3-HBA）to 3-hydroxybenzoate and catechol. Also genes(mhbDHIM), from Klebsiella pneumoniae M5a1 which is capable of utilizing 3-hydroxybenzoate, encode enzymes converting 3-hydroxybenzoate (3-HBA) to pyruvate and fumarate via gentisate.
Therefore, our project is to design a device with engineered bacterias which are capable of degrading 3-PBA more efficiently.
